THE ANTIBACTERIAL EFFECT OF ROSELLE (Hibiscus sabdariffa) EXTRACT AGAINST Staphylococcus epidermidis IN VITRO
Infection of Staphylococcus epidermidis is still a common problem in many hospitals. Factor determining biofilm formation makes it harder for atibiotics to cure the infection. Roselle (Hibiscus sabdariffa), a well known traditional medicine plant, is a potential candidate as a drug againts infectiou...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Airlangga
2017-08-01
|
| Series: | Indonesian Journal of Tropical and Infectious Disease |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/IJTID/article/view/3757 |
| _version_ | 1831543759594061824 |
|---|---|
| author | Terrence Timothy Evan Lusida Bambang Hermanto Sudarno Sudarno |
| author_facet | Terrence Timothy Evan Lusida Bambang Hermanto Sudarno Sudarno |
| author_sort | Terrence Timothy Evan Lusida |
| collection | DOAJ |
| description | Infection of Staphylococcus epidermidis is still a common problem in many hospitals. Factor determining biofilm formation makes it harder for atibiotics to cure the infection. Roselle (Hibiscus sabdariffa), a well known traditional medicine plant, is a potential candidate as a drug againts infectious disease. The purpose of this research is to investigate the antibacterial effect of ethanol extract from Roselle (Hibiscus sabdariffa) calyx againts the growth of Staphylococcus epidermidis. Assessment for antibacterial effect is performed using broth diffusion method. The extract is made by maceration of the calyx of Roselle in 96% ethanol. Extracts with concentration of 125, 62.5, 31.25, 15.63, 7.81, 3.90, 1.95, 0.97, 0.48, 0.24 mg/mL are added into separated Mueller-Hinton broths (MHB), which have already been inoculated by Staphylococcus epidermidis. As for bacterial growth control, we used MHB with bacterial inoculation, while sterility control we used mixture of extract and MHB. Then from each broth, the solutions are added into separated nutrition agar plates. Replications are done three times. Clarity and bacterial growth are observed after 24 hours of incubation. However, clarity cannot be observed in 36 broth, but bacterial growth is observed on the plate for concentration 0.97, 0.48, and 0.24 mg/mL. Therefore Minimum inhibitory concentration (MIC) cannot be determined because the extract’s color interfere the observation. While minimum bactericidal concentration (MBC), the last concentration before the concentration where the bacteria are still viable, is 1.95 mg/mL. Based on the result of the research, the Roselle calyx ethanol extract (Hibiscus sabdariffa) through dilution method with a concentration of 1.95 mg / mL can kill Staphylococcus epidermidis and in order to find MIC in collored and turbid solution (before being incubated in incubator), we can consider using agar dilution methode or microdilution methode. |
| first_indexed | 2024-12-17T00:56:47Z |
| format | Article |
| id | doaj.art-43d3d74b004441b38ab7eb1b8684b673 |
| institution | Directory Open Access Journal |
| issn | 2085-1103 2356-0991 |
| language | English |
| last_indexed | 2024-12-17T00:56:47Z |
| publishDate | 2017-08-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Indonesian Journal of Tropical and Infectious Disease |
| spelling | doaj.art-43d3d74b004441b38ab7eb1b8684b6732022-12-21T22:09:34ZengUniversitas AirlanggaIndonesian Journal of Tropical and Infectious Disease2085-11032356-09912017-08-0164889110.20473/ijtid.v6i4.37573350THE ANTIBACTERIAL EFFECT OF ROSELLE (Hibiscus sabdariffa) EXTRACT AGAINST Staphylococcus epidermidis IN VITROTerrence Timothy Evan Lusida0Bambang Hermanto1Sudarno Sudarno2Faculty of Medicine, Universitas Airlangga, SurabayaDepartment of Pharmacology, Faculty of Medicine, Universitas Airlangga, SurabayaDepartment of Biochemistry, Faculty of Medicine, Universitas Airlangga, SurabayaInfection of Staphylococcus epidermidis is still a common problem in many hospitals. Factor determining biofilm formation makes it harder for atibiotics to cure the infection. Roselle (Hibiscus sabdariffa), a well known traditional medicine plant, is a potential candidate as a drug againts infectious disease. The purpose of this research is to investigate the antibacterial effect of ethanol extract from Roselle (Hibiscus sabdariffa) calyx againts the growth of Staphylococcus epidermidis. Assessment for antibacterial effect is performed using broth diffusion method. The extract is made by maceration of the calyx of Roselle in 96% ethanol. Extracts with concentration of 125, 62.5, 31.25, 15.63, 7.81, 3.90, 1.95, 0.97, 0.48, 0.24 mg/mL are added into separated Mueller-Hinton broths (MHB), which have already been inoculated by Staphylococcus epidermidis. As for bacterial growth control, we used MHB with bacterial inoculation, while sterility control we used mixture of extract and MHB. Then from each broth, the solutions are added into separated nutrition agar plates. Replications are done three times. Clarity and bacterial growth are observed after 24 hours of incubation. However, clarity cannot be observed in 36 broth, but bacterial growth is observed on the plate for concentration 0.97, 0.48, and 0.24 mg/mL. Therefore Minimum inhibitory concentration (MIC) cannot be determined because the extract’s color interfere the observation. While minimum bactericidal concentration (MBC), the last concentration before the concentration where the bacteria are still viable, is 1.95 mg/mL. Based on the result of the research, the Roselle calyx ethanol extract (Hibiscus sabdariffa) through dilution method with a concentration of 1.95 mg / mL can kill Staphylococcus epidermidis and in order to find MIC in collored and turbid solution (before being incubated in incubator), we can consider using agar dilution methode or microdilution methode.https://e-journal.unair.ac.id/IJTID/article/view/3757hibiscus sabdariffaantibacterialstaphylococcus epidermidisbiofilmflavonoids |
| spellingShingle | Terrence Timothy Evan Lusida Bambang Hermanto Sudarno Sudarno THE ANTIBACTERIAL EFFECT OF ROSELLE (Hibiscus sabdariffa) EXTRACT AGAINST Staphylococcus epidermidis IN VITRO Indonesian Journal of Tropical and Infectious Disease hibiscus sabdariffa antibacterial staphylococcus epidermidis biofilm flavonoids |
| title | THE ANTIBACTERIAL EFFECT OF ROSELLE (Hibiscus sabdariffa) EXTRACT AGAINST Staphylococcus epidermidis IN VITRO |
| title_full | THE ANTIBACTERIAL EFFECT OF ROSELLE (Hibiscus sabdariffa) EXTRACT AGAINST Staphylococcus epidermidis IN VITRO |
| title_fullStr | THE ANTIBACTERIAL EFFECT OF ROSELLE (Hibiscus sabdariffa) EXTRACT AGAINST Staphylococcus epidermidis IN VITRO |
| title_full_unstemmed | THE ANTIBACTERIAL EFFECT OF ROSELLE (Hibiscus sabdariffa) EXTRACT AGAINST Staphylococcus epidermidis IN VITRO |
| title_short | THE ANTIBACTERIAL EFFECT OF ROSELLE (Hibiscus sabdariffa) EXTRACT AGAINST Staphylococcus epidermidis IN VITRO |
| title_sort | antibacterial effect of roselle hibiscus sabdariffa extract against staphylococcus epidermidis in vitro |
| topic | hibiscus sabdariffa antibacterial staphylococcus epidermidis biofilm flavonoids |
| url | https://e-journal.unair.ac.id/IJTID/article/view/3757 |
| work_keys_str_mv | AT terrencetimothyevanlusida theantibacterialeffectofrosellehibiscussabdariffaextractagainststaphylococcusepidermidisinvitro AT bambanghermanto theantibacterialeffectofrosellehibiscussabdariffaextractagainststaphylococcusepidermidisinvitro AT sudarnosudarno theantibacterialeffectofrosellehibiscussabdariffaextractagainststaphylococcusepidermidisinvitro AT terrencetimothyevanlusida antibacterialeffectofrosellehibiscussabdariffaextractagainststaphylococcusepidermidisinvitro AT bambanghermanto antibacterialeffectofrosellehibiscussabdariffaextractagainststaphylococcusepidermidisinvitro AT sudarnosudarno antibacterialeffectofrosellehibiscussabdariffaextractagainststaphylococcusepidermidisinvitro |