Combinations of Hydrostatic Pressure and Shear Stress Time-dependently Decrease E-selectin, VCAM-1 and ICAM-1 Expression Induced by Tumor Necrosis Factor-Alpha in Cultured Endothelial Cells
Endothelial cells (ECs) are constantly subjected to shear stress, a tangential stress generated by blood flow, and hydrostatic pressure, a normal stress generated by blood pressure. Using a perfusion system that enables the independent control of flow volume and pressure, we investigated the effect...
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| Format: | Article |
| Language: | English |
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The Japan Society of Mechanical Engineers
2012-03-01
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| Series: | Journal of Biomechanical Science and Engineering |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jbse/7/2/7_2_118/_pdf/-char/en |
| _version_ | 1828763042840576000 |
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| author | Hiromichi NAKADATE Eiichi SEKIZUKA Shigeru AOMURA Haruyuki MINAMITANI |
| author_facet | Hiromichi NAKADATE Eiichi SEKIZUKA Shigeru AOMURA Haruyuki MINAMITANI |
| author_sort | Hiromichi NAKADATE |
| collection | DOAJ |
| description | Endothelial cells (ECs) are constantly subjected to shear stress, a tangential stress generated by blood flow, and hydrostatic pressure, a normal stress generated by blood pressure. Using a perfusion system that enables the independent control of flow volume and pressure, we investigated the effect of this combined stress on adhesion molecule expression in cytokine-stimulated cultured ECs. Human umbilical vein endothelial cells were incubated with 25 ng/ml tumor necrosis factor-alpha (TNF-α) for 6 hours. Activated ECs were then exposed to pulsatile flow (a shear stress of 1.5 ± 0.3 Pa, and a hydrostatic pressure of 100 ± 20 mmHg) for 1—24 hours. After exposure to pulsatile flow, endothelial selectin (E-selectin), intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) were stained and observed using fluorescence microscopy. Results showed that E-selectin was expressed in approximately 30% of the total ECs after incubation with TNF-α, however, TNF-α-induced E-selectin expression was decreased after 1 hour of exposure to pulsatile flow resulting in E-selectin expression in under 10% of total ECs at 24 hours. VCAM-1 was expressed in approximately 75% of the total ECs after incubation with TNF-α, however, TNF-α-induced VCAM-1 expression was decreased after 6 hours of exposure to pulsatile flow resulting in VCAM-1 expression in approximately 40% of the total ECs at 24 hours. ICAM-1 was expressed in all ECs after incubation with TNF-α, however, TNF-α-induced ICAM-1 expression was decreased after 12 hours of exposure to pulsatile flow resulting in ICAM-1 expression in approximately 40% of the total ECs at 24 hours. These results suggest that combined stress time-dependently suppresses overexpression of cytokine-induced adhesion molecules in ECs. |
| first_indexed | 2024-12-11T01:54:41Z |
| format | Article |
| id | doaj.art-43d6b3022c8e468aab265cefd80fe33a |
| institution | Directory Open Access Journal |
| issn | 1880-9863 |
| language | English |
| last_indexed | 2024-12-11T01:54:41Z |
| publishDate | 2012-03-01 |
| publisher | The Japan Society of Mechanical Engineers |
| record_format | Article |
| series | Journal of Biomechanical Science and Engineering |
| spelling | doaj.art-43d6b3022c8e468aab265cefd80fe33a2022-12-22T01:24:40ZengThe Japan Society of Mechanical EngineersJournal of Biomechanical Science and Engineering1880-98632012-03-017211812910.1299/jbse.7.118jbseCombinations of Hydrostatic Pressure and Shear Stress Time-dependently Decrease E-selectin, VCAM-1 and ICAM-1 Expression Induced by Tumor Necrosis Factor-Alpha in Cultured Endothelial CellsHiromichi NAKADATE0Eiichi SEKIZUKA1Shigeru AOMURA2Haruyuki MINAMITANI3Graduate School of System Design, Tokyo Metropolitan UniversityDepartment of Internal Medicine, National Hospital Organization Saitama National HospitalGraduate School of System Design, Tokyo Metropolitan UniversityFaculty of Photonics Science, Chitose Institute of Science and TechnologyEndothelial cells (ECs) are constantly subjected to shear stress, a tangential stress generated by blood flow, and hydrostatic pressure, a normal stress generated by blood pressure. Using a perfusion system that enables the independent control of flow volume and pressure, we investigated the effect of this combined stress on adhesion molecule expression in cytokine-stimulated cultured ECs. Human umbilical vein endothelial cells were incubated with 25 ng/ml tumor necrosis factor-alpha (TNF-α) for 6 hours. Activated ECs were then exposed to pulsatile flow (a shear stress of 1.5 ± 0.3 Pa, and a hydrostatic pressure of 100 ± 20 mmHg) for 1—24 hours. After exposure to pulsatile flow, endothelial selectin (E-selectin), intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) were stained and observed using fluorescence microscopy. Results showed that E-selectin was expressed in approximately 30% of the total ECs after incubation with TNF-α, however, TNF-α-induced E-selectin expression was decreased after 1 hour of exposure to pulsatile flow resulting in E-selectin expression in under 10% of total ECs at 24 hours. VCAM-1 was expressed in approximately 75% of the total ECs after incubation with TNF-α, however, TNF-α-induced VCAM-1 expression was decreased after 6 hours of exposure to pulsatile flow resulting in VCAM-1 expression in approximately 40% of the total ECs at 24 hours. ICAM-1 was expressed in all ECs after incubation with TNF-α, however, TNF-α-induced ICAM-1 expression was decreased after 12 hours of exposure to pulsatile flow resulting in ICAM-1 expression in approximately 40% of the total ECs at 24 hours. These results suggest that combined stress time-dependently suppresses overexpression of cytokine-induced adhesion molecules in ECs.https://www.jstage.jst.go.jp/article/jbse/7/2/7_2_118/_pdf/-char/enendothelial cellshydrostatic pressureshear stresse-selectinvcam-1icam-1tnf-αimmunofluorescence |
| spellingShingle | Hiromichi NAKADATE Eiichi SEKIZUKA Shigeru AOMURA Haruyuki MINAMITANI Combinations of Hydrostatic Pressure and Shear Stress Time-dependently Decrease E-selectin, VCAM-1 and ICAM-1 Expression Induced by Tumor Necrosis Factor-Alpha in Cultured Endothelial Cells Journal of Biomechanical Science and Engineering endothelial cells hydrostatic pressure shear stress e-selectin vcam-1 icam-1 tnf-α immunofluorescence |
| title | Combinations of Hydrostatic Pressure and Shear Stress Time-dependently Decrease E-selectin, VCAM-1 and ICAM-1 Expression Induced by Tumor Necrosis Factor-Alpha in Cultured Endothelial Cells |
| title_full | Combinations of Hydrostatic Pressure and Shear Stress Time-dependently Decrease E-selectin, VCAM-1 and ICAM-1 Expression Induced by Tumor Necrosis Factor-Alpha in Cultured Endothelial Cells |
| title_fullStr | Combinations of Hydrostatic Pressure and Shear Stress Time-dependently Decrease E-selectin, VCAM-1 and ICAM-1 Expression Induced by Tumor Necrosis Factor-Alpha in Cultured Endothelial Cells |
| title_full_unstemmed | Combinations of Hydrostatic Pressure and Shear Stress Time-dependently Decrease E-selectin, VCAM-1 and ICAM-1 Expression Induced by Tumor Necrosis Factor-Alpha in Cultured Endothelial Cells |
| title_short | Combinations of Hydrostatic Pressure and Shear Stress Time-dependently Decrease E-selectin, VCAM-1 and ICAM-1 Expression Induced by Tumor Necrosis Factor-Alpha in Cultured Endothelial Cells |
| title_sort | combinations of hydrostatic pressure and shear stress time dependently decrease e selectin vcam 1 and icam 1 expression induced by tumor necrosis factor alpha in cultured endothelial cells |
| topic | endothelial cells hydrostatic pressure shear stress e-selectin vcam-1 icam-1 tnf-α immunofluorescence |
| url | https://www.jstage.jst.go.jp/article/jbse/7/2/7_2_118/_pdf/-char/en |
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