Evaluation, prediction and optimization the ultrasound-assisted extraction method using response surface methodology: antioxidant and biological properties of Stachys parviflora L.
Objective(s):To optimize the extraction method using response surface methodology, extract the phenolic compounds, and identify the antioxidant and biological properties of Stachys parviflora L. extracts. Materials and Methods: Maceration and ultrasound-assisted extraction (UAE) (4, 7, 10 min treat...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2016-05-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_6938_77ecfccc2dad9436b5384d8acb56a520.pdf |
| _version_ | 1818677596126183424 |
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| author | Davoud Salarbashi Samaneh Attaran Dowom Bibi Sedigheh Fazly Bazzaz Farhad Khanzadeh Vahid Soheili Ali Mohammadpour |
| author_facet | Davoud Salarbashi Samaneh Attaran Dowom Bibi Sedigheh Fazly Bazzaz Farhad Khanzadeh Vahid Soheili Ali Mohammadpour |
| author_sort | Davoud Salarbashi |
| collection | DOAJ |
| description | Objective(s):To optimize the extraction method using response surface methodology, extract the phenolic compounds, and identify the antioxidant and biological properties of Stachys parviflora L. extracts. Materials and Methods: Maceration and ultrasound-assisted extraction (UAE) (4, 7, 10 min treatment time, 40, 70, 100 % high-intensity and 60, 80, 100 % (v v-1) methanol purity) were applied to obtain the extracts. SEM was conducted to provide the microstructure of the extracted plant. MICs (colorimetric assay), MFCs (colony diameter), total phenolic content, total flavonoid content, radical scavenging capacity and extraction efficiency were determined. HPLC analysis was applied to measure the existent phenolic compounds. Results: A quadratic model (4 min treatment time, 74.5 % high-intensity and 74.2 % solvent purity) was suggested as the best (TPC: 20.89 mg GAE g-1 d.m., TFC: 6.22 mg QEs g-1 d.m., DPPH IC50: 21.86 µg ml-1 and EE: 113.65 mg g-1 d.m.) UAE extraction model. The optimized UAE extract was generally more effective against Gram-positive microorganisms (MIC: 10-20; MBC: 10-40 (mg ml-1)) than Gram-negative ones (MIC: 40; MBC: >40 (mg ml-1)). Moreover, it (MGI: 2.32-100 %) revealed more anti-mold activity than maceration (MGI: Conclusion: RSM optimization was successfully applied for UAE from S. parviflora. The considerable antioxidant and biological properties were attributed to the phenolic compounds. |
| first_indexed | 2024-12-17T09:01:53Z |
| format | Article |
| id | doaj.art-43da5d22765d45a5b85b4deda1c9db20 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-17T09:01:53Z |
| publishDate | 2016-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-43da5d22765d45a5b85b4deda1c9db202022-12-21T21:55:40ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742016-05-011955295416938Evaluation, prediction and optimization the ultrasound-assisted extraction method using response surface methodology: antioxidant and biological properties of Stachys parviflora L.Davoud Salarbashi0Samaneh Attaran Dowom1Bibi Sedigheh Fazly Bazzaz2Farhad Khanzadeh3Vahid Soheili4Ali Mohammadpour5School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran|Ferdowsi University of Mashhad, International campus, Department of Food Science and Technology, Mashhad, IranDepartment of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, IranSchool of Pharmacy; Biotechnology Research Center; Mashhad University of Medical Sciences, Mashhad, IranDepartment of Food Science and Technology, Sabzevar Branch, Islamic Azad University, Sabzevar, IranDepartment of Food and Drug Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranMedical Surgical Nursing, Social Determinant of Health Center, Gonabad University of Medical Sciences, Gonabad, IranObjective(s):To optimize the extraction method using response surface methodology, extract the phenolic compounds, and identify the antioxidant and biological properties of Stachys parviflora L. extracts. Materials and Methods: Maceration and ultrasound-assisted extraction (UAE) (4, 7, 10 min treatment time, 40, 70, 100 % high-intensity and 60, 80, 100 % (v v-1) methanol purity) were applied to obtain the extracts. SEM was conducted to provide the microstructure of the extracted plant. MICs (colorimetric assay), MFCs (colony diameter), total phenolic content, total flavonoid content, radical scavenging capacity and extraction efficiency were determined. HPLC analysis was applied to measure the existent phenolic compounds. Results: A quadratic model (4 min treatment time, 74.5 % high-intensity and 74.2 % solvent purity) was suggested as the best (TPC: 20.89 mg GAE g-1 d.m., TFC: 6.22 mg QEs g-1 d.m., DPPH IC50: 21.86 µg ml-1 and EE: 113.65 mg g-1 d.m.) UAE extraction model. The optimized UAE extract was generally more effective against Gram-positive microorganisms (MIC: 10-20; MBC: 10-40 (mg ml-1)) than Gram-negative ones (MIC: 40; MBC: >40 (mg ml-1)). Moreover, it (MGI: 2.32-100 %) revealed more anti-mold activity than maceration (MGI: Conclusion: RSM optimization was successfully applied for UAE from S. parviflora. The considerable antioxidant and biological properties were attributed to the phenolic compounds.http://ijbms.mums.ac.ir/article_6938_77ecfccc2dad9436b5384d8acb56a520.pdfAntioxidantBiological propertiesHPLCRSMStachys parviflora LUAE |
| spellingShingle | Davoud Salarbashi Samaneh Attaran Dowom Bibi Sedigheh Fazly Bazzaz Farhad Khanzadeh Vahid Soheili Ali Mohammadpour Evaluation, prediction and optimization the ultrasound-assisted extraction method using response surface methodology: antioxidant and biological properties of Stachys parviflora L. Iranian Journal of Basic Medical Sciences Antioxidant Biological properties HPLC RSM Stachys parviflora L UAE |
| title | Evaluation, prediction and optimization the ultrasound-assisted extraction method using response surface methodology: antioxidant and biological properties of Stachys parviflora L. |
| title_full | Evaluation, prediction and optimization the ultrasound-assisted extraction method using response surface methodology: antioxidant and biological properties of Stachys parviflora L. |
| title_fullStr | Evaluation, prediction and optimization the ultrasound-assisted extraction method using response surface methodology: antioxidant and biological properties of Stachys parviflora L. |
| title_full_unstemmed | Evaluation, prediction and optimization the ultrasound-assisted extraction method using response surface methodology: antioxidant and biological properties of Stachys parviflora L. |
| title_short | Evaluation, prediction and optimization the ultrasound-assisted extraction method using response surface methodology: antioxidant and biological properties of Stachys parviflora L. |
| title_sort | evaluation prediction and optimization the ultrasound assisted extraction method using response surface methodology antioxidant and biological properties of stachys parviflora l |
| topic | Antioxidant Biological properties HPLC RSM Stachys parviflora L UAE |
| url | http://ijbms.mums.ac.ir/article_6938_77ecfccc2dad9436b5384d8acb56a520.pdf |
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