Developing Efficient Thermophilic Cellulose Degrading Consortium for Glucose Production From Different Agro-Residues
In the present study, 11 cellulose degrading bacterial strains were isolated from water and soil samples of hot springs in the Chumathang village, Leh and Ladakh region, India. The isolated strains were identified as Bacillus subtilis, Bacillus aryabhattai, Bacillus stratosphericus, Bacillus altitud...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-07-01
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| Series: | Frontiers in Energy Research |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fenrg.2019.00061/full |
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| author | Saurabh Singh Durgesh Kumar Jaiswal Nallusamy Sivakumar Jay Prakash Verma |
| author_facet | Saurabh Singh Durgesh Kumar Jaiswal Nallusamy Sivakumar Jay Prakash Verma |
| author_sort | Saurabh Singh |
| collection | DOAJ |
| description | In the present study, 11 cellulose degrading bacterial strains were isolated from water and soil samples of hot springs in the Chumathang village, Leh and Ladakh region, India. The isolated strains were identified as Bacillus subtilis, Bacillus aryabhattai, Bacillus stratosphericus, Bacillus altitudinis, and Brevibacterium frigoritolerans by biochemical and molecular approaches. All the strains were evaluated for the total cellulase, endoglucanase, exoglucanase, and β-glucosidase enzyme activities. On the basis of overall individual cellulose degrading enzyme activities, three strains were selected to develop consortium to enhance their cellulase enzyme activities. The potent cellulose degraders B. stratosphericus BHUJPV-H5, B. subtilis BHUJPV-H19, and B. subtilis BHUJPV-H12 were selected for the consortium development. The effect of cellulase activities of bacterial strains were evaluated ranged up to 6.06 and 0.72 mg ml−1 glucose by agro-residues of sugarcane bagasse and wheat straw, respectively, after 1 h of incubation. Total cellulase enzyme activity of consortia was recorded two times higher than the individual organisms. These strains can be used for enhancing bioethanol production from lignocellulosic biomass, which can consequently boost biofuel production industry. |
| first_indexed | 2024-12-17T13:33:42Z |
| format | Article |
| id | doaj.art-43da6a5a5fb24f1587fd45e31db31e72 |
| institution | Directory Open Access Journal |
| issn | 2296-598X |
| language | English |
| last_indexed | 2024-12-17T13:33:42Z |
| publishDate | 2019-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Energy Research |
| spelling | doaj.art-43da6a5a5fb24f1587fd45e31db31e722022-12-21T21:46:29ZengFrontiers Media S.A.Frontiers in Energy Research2296-598X2019-07-01710.3389/fenrg.2019.00061456133Developing Efficient Thermophilic Cellulose Degrading Consortium for Glucose Production From Different Agro-ResiduesSaurabh Singh0Durgesh Kumar Jaiswal1Nallusamy Sivakumar2Jay Prakash Verma3Institute of Environment and Sustainable Development, Banaras Hindu University, Varanasi, IndiaInstitute of Environment and Sustainable Development, Banaras Hindu University, Varanasi, IndiaDepartment of Biology, College of Science, Sultan Qaboos University, Muscat, OmanInstitute of Environment and Sustainable Development, Banaras Hindu University, Varanasi, IndiaIn the present study, 11 cellulose degrading bacterial strains were isolated from water and soil samples of hot springs in the Chumathang village, Leh and Ladakh region, India. The isolated strains were identified as Bacillus subtilis, Bacillus aryabhattai, Bacillus stratosphericus, Bacillus altitudinis, and Brevibacterium frigoritolerans by biochemical and molecular approaches. All the strains were evaluated for the total cellulase, endoglucanase, exoglucanase, and β-glucosidase enzyme activities. On the basis of overall individual cellulose degrading enzyme activities, three strains were selected to develop consortium to enhance their cellulase enzyme activities. The potent cellulose degraders B. stratosphericus BHUJPV-H5, B. subtilis BHUJPV-H19, and B. subtilis BHUJPV-H12 were selected for the consortium development. The effect of cellulase activities of bacterial strains were evaluated ranged up to 6.06 and 0.72 mg ml−1 glucose by agro-residues of sugarcane bagasse and wheat straw, respectively, after 1 h of incubation. Total cellulase enzyme activity of consortia was recorded two times higher than the individual organisms. These strains can be used for enhancing bioethanol production from lignocellulosic biomass, which can consequently boost biofuel production industry.https://www.frontiersin.org/article/10.3389/fenrg.2019.00061/fullcellulaseendoglucanaseexoglucanaseβ-glucosidasesugarcane bagassewheat straw |
| spellingShingle | Saurabh Singh Durgesh Kumar Jaiswal Nallusamy Sivakumar Jay Prakash Verma Developing Efficient Thermophilic Cellulose Degrading Consortium for Glucose Production From Different Agro-Residues Frontiers in Energy Research cellulase endoglucanase exoglucanase β-glucosidase sugarcane bagasse wheat straw |
| title | Developing Efficient Thermophilic Cellulose Degrading Consortium for Glucose Production From Different Agro-Residues |
| title_full | Developing Efficient Thermophilic Cellulose Degrading Consortium for Glucose Production From Different Agro-Residues |
| title_fullStr | Developing Efficient Thermophilic Cellulose Degrading Consortium for Glucose Production From Different Agro-Residues |
| title_full_unstemmed | Developing Efficient Thermophilic Cellulose Degrading Consortium for Glucose Production From Different Agro-Residues |
| title_short | Developing Efficient Thermophilic Cellulose Degrading Consortium for Glucose Production From Different Agro-Residues |
| title_sort | developing efficient thermophilic cellulose degrading consortium for glucose production from different agro residues |
| topic | cellulase endoglucanase exoglucanase β-glucosidase sugarcane bagasse wheat straw |
| url | https://www.frontiersin.org/article/10.3389/fenrg.2019.00061/full |
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