Phytochemical Composition and Bioassay on Iranian Teucrium polium Ex-tracts against Anopheles stephensi (Diptera: Culicidae)
Background: Anopheles stephensi is an important malaria vector mosquito in Iran and other western Asian countries. In many human communities, plant products have been used traditionally instead of synthetic pesticides for mosquito control due to their minimal hazardous effects. Teucrium polium, kno...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2023-01-01
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| Series: | Journal of Arthropod-Borne Diseases |
| Subjects: | |
| Online Access: | https://jad.tums.ac.ir/index.php/jad/article/view/1207 |
| _version_ | 1797902708386037760 |
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| author | Saeedeh Ghafari Azar Tahghighi Khadijeh Shamakhte Hamzeh Alipour Naseh Maleki-Ravasan Mehdi Nateghpour |
| author_facet | Saeedeh Ghafari Azar Tahghighi Khadijeh Shamakhte Hamzeh Alipour Naseh Maleki-Ravasan Mehdi Nateghpour |
| author_sort | Saeedeh Ghafari |
| collection | DOAJ |
| description |
Background: Anopheles stephensi is an important malaria vector mosquito in Iran and other western Asian countries. In many human communities, plant products have been used traditionally instead of synthetic pesticides for mosquito control due to their minimal hazardous effects. Teucrium polium, known popularly as felty germander, has been intro-duced in Persian Medicine (PM) as an insect repellent from a long time ago.
Methods: The present study was undertaken to evaluate repellent and larvicidal activity of dichloromethane (DCME-TP) and ethanolic extracts (EE-TP) of T. polium against An. stephensi under laboratory conditions. The possible chemi-cal components of the extracts were also investigated through gas chromatography/mass spectrometry (GC-MS) tech-nique.
Results: Based on the results, DCME-TP showed better repellent activity than EE-TP with 56.67 and 28.33 % protec-tion, respectively. Larvicidal activity of DCME-TP with 49.41% mortality was also higher than EE-TP (20.24%). The main identified constituents of DCME-TP were long chain alkanes, phenol, aromatic ester, oxaspiro and triterpenoid. While phenolic and aliphatic acid were only the identified components in EE-TP. It is notable that lupeol was detected in DCME of T. polium for the first time.
Conclusion: DCME-TP can be considered as a new herbal candidate to control An. stephensi mosquitoes. Further studies are required on this extract for the fractionation and identification of the active compounds, and the evaluation of their bioactivity in the laboratory and field.
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| first_indexed | 2024-04-10T09:21:54Z |
| format | Article |
| id | doaj.art-43db5a52bc614338b93bd26e223d0ee7 |
| institution | Directory Open Access Journal |
| issn | 2322-1984 2322-2271 |
| language | English |
| last_indexed | 2024-04-10T09:21:54Z |
| publishDate | 2023-01-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Arthropod-Borne Diseases |
| spelling | doaj.art-43db5a52bc614338b93bd26e223d0ee72023-02-20T08:54:27ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases2322-19842322-22712023-01-0116210.18502/jad.v16i2.11804Phytochemical Composition and Bioassay on Iranian Teucrium polium Ex-tracts against Anopheles stephensi (Diptera: Culicidae)Saeedeh Ghafari0Azar Tahghighi1Khadijeh Shamakhte2Hamzeh Alipour3Naseh Maleki-Ravasan4Mehdi Nateghpour5Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranMedicinal Chemistry Laboratory, Clinical Research Department, Pasteur Institute of Iran, Tehran, IranDepartment of Biochemistry, Payame Noor University, Tehran, IranResearch Center for Health Sciences, Institute of Health, Shiraz University of Medical Sciences, Shi-raz, IranDepartment of Parasitology, Pasteur Institute of Iran, Tehran, IranDepartment of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Background: Anopheles stephensi is an important malaria vector mosquito in Iran and other western Asian countries. In many human communities, plant products have been used traditionally instead of synthetic pesticides for mosquito control due to their minimal hazardous effects. Teucrium polium, known popularly as felty germander, has been intro-duced in Persian Medicine (PM) as an insect repellent from a long time ago. Methods: The present study was undertaken to evaluate repellent and larvicidal activity of dichloromethane (DCME-TP) and ethanolic extracts (EE-TP) of T. polium against An. stephensi under laboratory conditions. The possible chemi-cal components of the extracts were also investigated through gas chromatography/mass spectrometry (GC-MS) tech-nique. Results: Based on the results, DCME-TP showed better repellent activity than EE-TP with 56.67 and 28.33 % protec-tion, respectively. Larvicidal activity of DCME-TP with 49.41% mortality was also higher than EE-TP (20.24%). The main identified constituents of DCME-TP were long chain alkanes, phenol, aromatic ester, oxaspiro and triterpenoid. While phenolic and aliphatic acid were only the identified components in EE-TP. It is notable that lupeol was detected in DCME of T. polium for the first time. Conclusion: DCME-TP can be considered as a new herbal candidate to control An. stephensi mosquitoes. Further studies are required on this extract for the fractionation and identification of the active compounds, and the evaluation of their bioactivity in the laboratory and field. https://jad.tums.ac.ir/index.php/jad/article/view/1207LarvicidalRepellentTeucrium poliumAnopheles stephensiPhytochemical |
| spellingShingle | Saeedeh Ghafari Azar Tahghighi Khadijeh Shamakhte Hamzeh Alipour Naseh Maleki-Ravasan Mehdi Nateghpour Phytochemical Composition and Bioassay on Iranian Teucrium polium Ex-tracts against Anopheles stephensi (Diptera: Culicidae) Journal of Arthropod-Borne Diseases Larvicidal Repellent Teucrium polium Anopheles stephensi Phytochemical |
| title | Phytochemical Composition and Bioassay on Iranian Teucrium polium Ex-tracts against Anopheles stephensi (Diptera: Culicidae) |
| title_full | Phytochemical Composition and Bioassay on Iranian Teucrium polium Ex-tracts against Anopheles stephensi (Diptera: Culicidae) |
| title_fullStr | Phytochemical Composition and Bioassay on Iranian Teucrium polium Ex-tracts against Anopheles stephensi (Diptera: Culicidae) |
| title_full_unstemmed | Phytochemical Composition and Bioassay on Iranian Teucrium polium Ex-tracts against Anopheles stephensi (Diptera: Culicidae) |
| title_short | Phytochemical Composition and Bioassay on Iranian Teucrium polium Ex-tracts against Anopheles stephensi (Diptera: Culicidae) |
| title_sort | phytochemical composition and bioassay on iranian teucrium polium ex tracts against anopheles stephensi diptera culicidae |
| topic | Larvicidal Repellent Teucrium polium Anopheles stephensi Phytochemical |
| url | https://jad.tums.ac.ir/index.php/jad/article/view/1207 |
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