Thonningia sanguinea Vahl. (Balanophoraceae) in Southern Nigeria: II. Patterns of Genetic Diversity and Population Structure within and between Populations
Studies have yet to assess the genetic variability in Thonningia sanguinea populations in Southern Nigeria. Hence, this study was conducted to elucidate the pattern of genetic variability and population structure among T. sanguinea populations in Southern Nigeria. Genomic DNA was extracted from 31...
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2019-12-01
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| Series: | Makara Journal of Science |
| Subjects: | |
| Online Access: | https://scholarhub.ui.ac.id/cgi/viewcontent.cgi?article=1147&context=science |
| _version_ | 1818152585084796928 |
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| author | Odoligie Imarhiagbe Emmanuel Izaka Aigbokhan |
| author_facet | Odoligie Imarhiagbe Emmanuel Izaka Aigbokhan |
| author_sort | Odoligie Imarhiagbe |
| collection | DOAJ |
| description | Studies have yet to assess the genetic variability in Thonningia sanguinea populations in Southern Nigeria. Hence, this
study was conducted to elucidate the pattern of genetic variability and population structure among T. sanguinea populations in Southern Nigeria. Genomic DNA was extracted from 31 individuals in 15 populations and tested using random
amplified polymorphic DNA (RAPD) primers. Several genetic diversity parameters were examined using GenALEx
Ver. 6.5. Reproducible RAPD markers indicated that all the sampled populations were composed of individuals with a
high genetic variability. The populations were grouped into four distinct clusters. The populations from the Okour
community forest had high gene diversity and Shannon index. Conversely, the populations from Cross River National
Park had the lowest gene diversity and Shannon index. Genetic variability did not correlate with geographic distances.
Analysis of molecular variance revealed that most (82.3%) of the diversities could be explained by allelic variations
within the population. An indirect estimate of gene flow yielded gave an Nm of 1.09, indicating a low migration level among populations. Results demonstrated that T. sanguinea populations in Southern Nigeria exhibited outcrossing strategy expected of the sexual exchange of gametes by different individuals within a local population. |
| first_indexed | 2024-12-11T13:57:03Z |
| format | Article |
| id | doaj.art-43df2379ee5e45a9913c6af107c114a0 |
| institution | Directory Open Access Journal |
| issn | 2339-1995 2356-0851 |
| language | English |
| last_indexed | 2024-12-11T13:57:03Z |
| publishDate | 2019-12-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Makara Journal of Science |
| spelling | doaj.art-43df2379ee5e45a9913c6af107c114a02022-12-22T01:04:01ZengUniversitas IndonesiaMakara Journal of Science2339-19952356-08512019-12-0123419320310.7454/mss.v23i4.11510Thonningia sanguinea Vahl. (Balanophoraceae) in Southern Nigeria: II. Patterns of Genetic Diversity and Population Structure within and between PopulationsOdoligie Imarhiagbe 0Emmanuel Izaka Aigbokhan 1Department of Biological Science, Edo University Iyamho, Edo State 300271, NigeriaDepartment of Plant Biology and Biotechnology, University of Benin, Benin City 300211, Nigeria Studies have yet to assess the genetic variability in Thonningia sanguinea populations in Southern Nigeria. Hence, this study was conducted to elucidate the pattern of genetic variability and population structure among T. sanguinea populations in Southern Nigeria. Genomic DNA was extracted from 31 individuals in 15 populations and tested using random amplified polymorphic DNA (RAPD) primers. Several genetic diversity parameters were examined using GenALEx Ver. 6.5. Reproducible RAPD markers indicated that all the sampled populations were composed of individuals with a high genetic variability. The populations were grouped into four distinct clusters. The populations from the Okour community forest had high gene diversity and Shannon index. Conversely, the populations from Cross River National Park had the lowest gene diversity and Shannon index. Genetic variability did not correlate with geographic distances. Analysis of molecular variance revealed that most (82.3%) of the diversities could be explained by allelic variations within the population. An indirect estimate of gene flow yielded gave an Nm of 1.09, indicating a low migration level among populations. Results demonstrated that T. sanguinea populations in Southern Nigeria exhibited outcrossing strategy expected of the sexual exchange of gametes by different individuals within a local population.https://scholarhub.ui.ac.id/cgi/viewcontent.cgi?article=1147&context=scienceparasitic plantsrapdamovathonningia sanguineabalanophoraceae |
| spellingShingle | Odoligie Imarhiagbe Emmanuel Izaka Aigbokhan Thonningia sanguinea Vahl. (Balanophoraceae) in Southern Nigeria: II. Patterns of Genetic Diversity and Population Structure within and between Populations Makara Journal of Science parasitic plants rapd amova thonningia sanguinea balanophoraceae |
| title | Thonningia sanguinea Vahl. (Balanophoraceae) in Southern Nigeria: II. Patterns of Genetic Diversity and Population Structure within and between Populations |
| title_full | Thonningia sanguinea Vahl. (Balanophoraceae) in Southern Nigeria: II. Patterns of Genetic Diversity and Population Structure within and between Populations |
| title_fullStr | Thonningia sanguinea Vahl. (Balanophoraceae) in Southern Nigeria: II. Patterns of Genetic Diversity and Population Structure within and between Populations |
| title_full_unstemmed | Thonningia sanguinea Vahl. (Balanophoraceae) in Southern Nigeria: II. Patterns of Genetic Diversity and Population Structure within and between Populations |
| title_short | Thonningia sanguinea Vahl. (Balanophoraceae) in Southern Nigeria: II. Patterns of Genetic Diversity and Population Structure within and between Populations |
| title_sort | thonningia sanguinea vahl balanophoraceae in southern nigeria ii patterns of genetic diversity and population structure within and between populations |
| topic | parasitic plants rapd amova thonningia sanguinea balanophoraceae |
| url | https://scholarhub.ui.ac.id/cgi/viewcontent.cgi?article=1147&context=science |
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