Oleuropein Enhances Stress Resistance and Extends Lifespan via Insulin/IGF-1 and SKN-1/Nrf2 Signaling Pathway in <i>Caenorhabditis elegans</i>
Oleuropein (OLE) is a secoiridoid glycoside that mainly exists in olives with multifaceted health benefits. The present study aimed to investigate the stress resistance and lifespan extension effects of OLE in <i>Caenorhabditis elegans</i>. The results showed that OLE could significantly...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/10/11/1697 |
| _version_ | 1827677511337639936 |
|---|---|
| author | Shiling Feng Chunyan Zhang Tao Chen Lijun Zhou Yan Huang Ming Yuan Tian Li Chunbang Ding |
| author_facet | Shiling Feng Chunyan Zhang Tao Chen Lijun Zhou Yan Huang Ming Yuan Tian Li Chunbang Ding |
| author_sort | Shiling Feng |
| collection | DOAJ |
| description | Oleuropein (OLE) is a secoiridoid glycoside that mainly exists in olives with multifaceted health benefits. The present study aimed to investigate the stress resistance and lifespan extension effects of OLE in <i>Caenorhabditis elegans</i>. The results showed that OLE could significantly prolong the lifespan of <i>C. elegans</i> by 22.29%. Treatment with OLE also significantly increased the survival rates of worms against lethal heat shock and oxidative stress. Meanwhile, OLE supplementation increased the expression and activity of antioxidant enzymes and suppressed the generation of malondialdehyde in nematodes. In addition, the results from mutants implied that OLE might mediate longevity and stress resistance via DAF-16/FoxO, which played a vital role in the insulin/IGF-1 signaling (IIS) pathway. To further identify the molecular targets of OLE, mRNA level and loss-of-function mutants of IIS-associated genes were investigated. The data revealed that OLE activated IIS by down-regulating the upstream components, <i>daf-2</i> and <i>age-1</i>. Furthermore, another stress response and longevity pathway in parallel to DAF-16, SKN-1/Nrf2, was also shown to involve in OLE-induced beneficial effects. Collectively, these results provide the theoretical basis that OLE could enhance the stress resistance and increase the lifespan of <i>C. elegans</i> through the IIS and SKN-1/Nrf2 signaling pathways. |
| first_indexed | 2024-03-10T05:45:01Z |
| format | Article |
| id | doaj.art-43df9a24c9fd4f42838a20840e397528 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T05:45:01Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-43df9a24c9fd4f42838a20840e3975282023-11-22T22:12:22ZengMDPI AGAntioxidants2076-39212021-10-011011169710.3390/antiox10111697Oleuropein Enhances Stress Resistance and Extends Lifespan via Insulin/IGF-1 and SKN-1/Nrf2 Signaling Pathway in <i>Caenorhabditis elegans</i>Shiling Feng0Chunyan Zhang1Tao Chen2Lijun Zhou3Yan Huang4Ming Yuan5Tian Li6Chunbang Ding7College of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Agronomy Science, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaOleuropein (OLE) is a secoiridoid glycoside that mainly exists in olives with multifaceted health benefits. The present study aimed to investigate the stress resistance and lifespan extension effects of OLE in <i>Caenorhabditis elegans</i>. The results showed that OLE could significantly prolong the lifespan of <i>C. elegans</i> by 22.29%. Treatment with OLE also significantly increased the survival rates of worms against lethal heat shock and oxidative stress. Meanwhile, OLE supplementation increased the expression and activity of antioxidant enzymes and suppressed the generation of malondialdehyde in nematodes. In addition, the results from mutants implied that OLE might mediate longevity and stress resistance via DAF-16/FoxO, which played a vital role in the insulin/IGF-1 signaling (IIS) pathway. To further identify the molecular targets of OLE, mRNA level and loss-of-function mutants of IIS-associated genes were investigated. The data revealed that OLE activated IIS by down-regulating the upstream components, <i>daf-2</i> and <i>age-1</i>. Furthermore, another stress response and longevity pathway in parallel to DAF-16, SKN-1/Nrf2, was also shown to involve in OLE-induced beneficial effects. Collectively, these results provide the theoretical basis that OLE could enhance the stress resistance and increase the lifespan of <i>C. elegans</i> through the IIS and SKN-1/Nrf2 signaling pathways.https://www.mdpi.com/2076-3921/10/11/1697oleuropein<i>Caenorhabditis elegans</i>longevitystress resistance |
| spellingShingle | Shiling Feng Chunyan Zhang Tao Chen Lijun Zhou Yan Huang Ming Yuan Tian Li Chunbang Ding Oleuropein Enhances Stress Resistance and Extends Lifespan via Insulin/IGF-1 and SKN-1/Nrf2 Signaling Pathway in <i>Caenorhabditis elegans</i> Antioxidants oleuropein <i>Caenorhabditis elegans</i> longevity stress resistance |
| title | Oleuropein Enhances Stress Resistance and Extends Lifespan via Insulin/IGF-1 and SKN-1/Nrf2 Signaling Pathway in <i>Caenorhabditis elegans</i> |
| title_full | Oleuropein Enhances Stress Resistance and Extends Lifespan via Insulin/IGF-1 and SKN-1/Nrf2 Signaling Pathway in <i>Caenorhabditis elegans</i> |
| title_fullStr | Oleuropein Enhances Stress Resistance and Extends Lifespan via Insulin/IGF-1 and SKN-1/Nrf2 Signaling Pathway in <i>Caenorhabditis elegans</i> |
| title_full_unstemmed | Oleuropein Enhances Stress Resistance and Extends Lifespan via Insulin/IGF-1 and SKN-1/Nrf2 Signaling Pathway in <i>Caenorhabditis elegans</i> |
| title_short | Oleuropein Enhances Stress Resistance and Extends Lifespan via Insulin/IGF-1 and SKN-1/Nrf2 Signaling Pathway in <i>Caenorhabditis elegans</i> |
| title_sort | oleuropein enhances stress resistance and extends lifespan via insulin igf 1 and skn 1 nrf2 signaling pathway in i caenorhabditis elegans i |
| topic | oleuropein <i>Caenorhabditis elegans</i> longevity stress resistance |
| url | https://www.mdpi.com/2076-3921/10/11/1697 |
| work_keys_str_mv | AT shilingfeng oleuropeinenhancesstressresistanceandextendslifespanviainsulinigf1andskn1nrf2signalingpathwayinicaenorhabditiselegansi AT chunyanzhang oleuropeinenhancesstressresistanceandextendslifespanviainsulinigf1andskn1nrf2signalingpathwayinicaenorhabditiselegansi AT taochen oleuropeinenhancesstressresistanceandextendslifespanviainsulinigf1andskn1nrf2signalingpathwayinicaenorhabditiselegansi AT lijunzhou oleuropeinenhancesstressresistanceandextendslifespanviainsulinigf1andskn1nrf2signalingpathwayinicaenorhabditiselegansi AT yanhuang oleuropeinenhancesstressresistanceandextendslifespanviainsulinigf1andskn1nrf2signalingpathwayinicaenorhabditiselegansi AT mingyuan oleuropeinenhancesstressresistanceandextendslifespanviainsulinigf1andskn1nrf2signalingpathwayinicaenorhabditiselegansi AT tianli oleuropeinenhancesstressresistanceandextendslifespanviainsulinigf1andskn1nrf2signalingpathwayinicaenorhabditiselegansi AT chunbangding oleuropeinenhancesstressresistanceandextendslifespanviainsulinigf1andskn1nrf2signalingpathwayinicaenorhabditiselegansi |