Human Bone Marrow Mesenchymal Stem Cells Promote the M2 Phenotype in Macrophages Derived from STEMI Patients
Acute ST-elevation myocardial infarction (STEMI) leads to myocardial injury or necrosis, and M1 macrophages play an important role in the inflammatory response. Bone marrow mesenchymal stem/stromal cells (BM-MSCs) are capable of modulating macrophage plasticity, principally due to their immunoregula...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/22/16257 |
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| author | Víctor Adrián Cortés-Morales Wendy Guadalupe Vázquez-González Juan José Montesinos Luis Moreno-Ruíz Selene Salgado-Pastor Pamela Michelle Salinas-Arreola Karla Díaz-Duarte Adriana Karina Chávez-Rueda Luis Chávez-Sánchez |
| author_facet | Víctor Adrián Cortés-Morales Wendy Guadalupe Vázquez-González Juan José Montesinos Luis Moreno-Ruíz Selene Salgado-Pastor Pamela Michelle Salinas-Arreola Karla Díaz-Duarte Adriana Karina Chávez-Rueda Luis Chávez-Sánchez |
| author_sort | Víctor Adrián Cortés-Morales |
| collection | DOAJ |
| description | Acute ST-elevation myocardial infarction (STEMI) leads to myocardial injury or necrosis, and M1 macrophages play an important role in the inflammatory response. Bone marrow mesenchymal stem/stromal cells (BM-MSCs) are capable of modulating macrophage plasticity, principally due to their immunoregulatory capacity. In the present study, we analyzed the capacity of MSCs to modulate macrophages derived from monocytes from patients with STEMI. We analyzed the circulating levels of cytokines associated with M1 and M2 macrophages in patients with STEMI, and the levels of cytokines associated with M1 macrophages were significantly higher in patients with STEMI than in controls. BM-MSCs facilitate the generation of M1 and M2 macrophages. M1 macrophages cocultured with MSCs did not have decreased M1 marker expression, but these macrophages had an increased expression of markers of the M2 macrophage phenotype (CD14, CD163 and CD206) and IL-10 and IL-1Ra signaling-induced regulatory T cells (Tregs). M2 macrophages from patients with STEMI had an increased expression of M2 phenotypic markers in coculture with BM-MSCs, as well as an increased secretion of anti-inflammatory cytokines and an increased generation of Tregs. The findings in this study indicate that BM-MSCs have the ability to modulate the M1 macrophage response, which could improve cardiac tissue damage in patients with STEMI. |
| first_indexed | 2024-03-09T16:45:48Z |
| format | Article |
| id | doaj.art-43e1a2af52014a32a46752bd65d972b1 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:45:48Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-43e1a2af52014a32a46752bd65d972b12023-11-24T14:46:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124221625710.3390/ijms242216257Human Bone Marrow Mesenchymal Stem Cells Promote the M2 Phenotype in Macrophages Derived from STEMI PatientsVíctor Adrián Cortés-Morales0Wendy Guadalupe Vázquez-González1Juan José Montesinos2Luis Moreno-Ruíz3Selene Salgado-Pastor4Pamela Michelle Salinas-Arreola5Karla Díaz-Duarte6Adriana Karina Chávez-Rueda7Luis Chávez-Sánchez8Unidad de Investigación Médica en Inmunoquímica, Hospital de Especialidades, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City 06720, MexicoUnidad de Investigación Médica en Enfermedades Metabólicas del Hospital de Cardiología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City 06720, MexicoUnidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City 06720, MexicoDivisión de Cardiología del Hospital de Cardiología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City 06720, MexicoDivisión de Cardiología del Hospital de Cardiología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City 06720, MexicoUnidad de Investigación Médica en Enfermedades Metabólicas del Hospital de Cardiología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City 06720, MexicoUnidad de Investigación Médica en Enfermedades Metabólicas del Hospital de Cardiología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City 06720, MexicoUnidad de Investigación Médica en Inmunología, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City 06720, MexicoUnidad de Investigación Médica en Enfermedades Metabólicas del Hospital de Cardiología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City 06720, MexicoAcute ST-elevation myocardial infarction (STEMI) leads to myocardial injury or necrosis, and M1 macrophages play an important role in the inflammatory response. Bone marrow mesenchymal stem/stromal cells (BM-MSCs) are capable of modulating macrophage plasticity, principally due to their immunoregulatory capacity. In the present study, we analyzed the capacity of MSCs to modulate macrophages derived from monocytes from patients with STEMI. We analyzed the circulating levels of cytokines associated with M1 and M2 macrophages in patients with STEMI, and the levels of cytokines associated with M1 macrophages were significantly higher in patients with STEMI than in controls. BM-MSCs facilitate the generation of M1 and M2 macrophages. M1 macrophages cocultured with MSCs did not have decreased M1 marker expression, but these macrophages had an increased expression of markers of the M2 macrophage phenotype (CD14, CD163 and CD206) and IL-10 and IL-1Ra signaling-induced regulatory T cells (Tregs). M2 macrophages from patients with STEMI had an increased expression of M2 phenotypic markers in coculture with BM-MSCs, as well as an increased secretion of anti-inflammatory cytokines and an increased generation of Tregs. The findings in this study indicate that BM-MSCs have the ability to modulate the M1 macrophage response, which could improve cardiac tissue damage in patients with STEMI.https://www.mdpi.com/1422-0067/24/22/16257acute ST-elevation myocardial infarctionbone marrow mesenchymal stem/stromal cellsM1 macrophagesM2 macrophagesregulatory T-cells |
| spellingShingle | Víctor Adrián Cortés-Morales Wendy Guadalupe Vázquez-González Juan José Montesinos Luis Moreno-Ruíz Selene Salgado-Pastor Pamela Michelle Salinas-Arreola Karla Díaz-Duarte Adriana Karina Chávez-Rueda Luis Chávez-Sánchez Human Bone Marrow Mesenchymal Stem Cells Promote the M2 Phenotype in Macrophages Derived from STEMI Patients International Journal of Molecular Sciences acute ST-elevation myocardial infarction bone marrow mesenchymal stem/stromal cells M1 macrophages M2 macrophages regulatory T-cells |
| title | Human Bone Marrow Mesenchymal Stem Cells Promote the M2 Phenotype in Macrophages Derived from STEMI Patients |
| title_full | Human Bone Marrow Mesenchymal Stem Cells Promote the M2 Phenotype in Macrophages Derived from STEMI Patients |
| title_fullStr | Human Bone Marrow Mesenchymal Stem Cells Promote the M2 Phenotype in Macrophages Derived from STEMI Patients |
| title_full_unstemmed | Human Bone Marrow Mesenchymal Stem Cells Promote the M2 Phenotype in Macrophages Derived from STEMI Patients |
| title_short | Human Bone Marrow Mesenchymal Stem Cells Promote the M2 Phenotype in Macrophages Derived from STEMI Patients |
| title_sort | human bone marrow mesenchymal stem cells promote the m2 phenotype in macrophages derived from stemi patients |
| topic | acute ST-elevation myocardial infarction bone marrow mesenchymal stem/stromal cells M1 macrophages M2 macrophages regulatory T-cells |
| url | https://www.mdpi.com/1422-0067/24/22/16257 |
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