Comparative transcriptomic and metabonomic analysis revealed the relationships between biosynthesis of volatiles and flavonoid metabolites in Rosa rugosa
Rosa rugosa is not only cultivated as a landscaping plant, but also used in cosmetics, the medical and food industries. However, little information is currently available on the gene regulatory networks involved in its scent and color biosynthesis and metabolism. In this study, R. rugosa Thunb. f. r...
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| Format: | Article |
| Language: | English |
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Maximum Academic Press
2021-01-01
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| Series: | Ornamental Plant Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/OPR-2021-0005 |
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| author | Dedang Feng Hao Zhang Xianqin Qiu Hongying Jian Qigang Wang Ningning Zhou Yu Ye Jun Lu Huijun Yan Kaixue Tang |
| author_facet | Dedang Feng Hao Zhang Xianqin Qiu Hongying Jian Qigang Wang Ningning Zhou Yu Ye Jun Lu Huijun Yan Kaixue Tang |
| author_sort | Dedang Feng |
| collection | DOAJ |
| description | Rosa rugosa is not only cultivated as a landscaping plant, but also used in cosmetics, the medical and food industries. However, little information is currently available on the gene regulatory networks involved in its scent and color biosynthesis and metabolism. In this study, R. rugosa Thunb. f. rosea Rehd with red petals (RR) and its white petal variant (WR), were used to study the molecular mechanisms in flower color and scent. Sixty-five differential flavonoid metabolites and 15 volatiles were found to have significant differences between RR and WR. Correspondingly, the key regulators (MYB-bHLH-WD40) of anthocyanin synthesis pathway and their structural genes involved in flavonoid biosynthesis, benzenoid/phenylpropanoid biosynthesis, terpenoid biosynthesis pathways were also found to be differentially expressed by comparative transcriptome. Further, qPCR permitted the identification of some transcripts encoding proteins that were putatively associated with scent and color biosynthesis in roses. Particularly, the results showed that the ACT gene (encoding CoA geraniol/citronellol acetyltransferase, GeneID: 112190420), which expressed lower in WR, was involved in three pathways: flavonoid biosynthesis, phenylpropanoid biosynthesis and terpenoid biosynthesis, however, GT5 (anthocyanin glycosylation gene, GeneID:112186660), expressed higher in WR, was involved in both flavonoid and phenylpropanoid biosynthesis pathways. These results suggested that ACT and GT5 might play important roles in regulating the relationship of color pigmentation and volatile emission. |
| first_indexed | 2024-03-07T20:09:47Z |
| format | Article |
| id | doaj.art-43e438b26ce9446e9912a857f389a56e |
| institution | Directory Open Access Journal |
| issn | 2769-2094 |
| language | English |
| last_indexed | 2024-03-07T20:09:47Z |
| publishDate | 2021-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Ornamental Plant Research |
| spelling | doaj.art-43e438b26ce9446e9912a857f389a56e2024-02-28T01:52:27ZengMaximum Academic PressOrnamental Plant Research2769-20942021-01-011111010.48130/OPR-2021-0005OPR-2021-0005Comparative transcriptomic and metabonomic analysis revealed the relationships between biosynthesis of volatiles and flavonoid metabolites in Rosa rugosaDedang Feng0Hao Zhang1Xianqin Qiu2Hongying Jian3Qigang Wang4Ningning Zhou5Yu Ye6Jun Lu7Huijun Yan8Kaixue Tang9College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, P. R. ChinaFlower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, P. R. ChinaFlower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, P. R. ChinaFlower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, P. R. ChinaFlower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, P. R. ChinaFlower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, P. R. ChinaKunming Academy of Agricultural Sciences, Kunming 650034, Yunnan, P. R. ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaFlower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, P. R. ChinaFlower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, P. R. ChinaRosa rugosa is not only cultivated as a landscaping plant, but also used in cosmetics, the medical and food industries. However, little information is currently available on the gene regulatory networks involved in its scent and color biosynthesis and metabolism. In this study, R. rugosa Thunb. f. rosea Rehd with red petals (RR) and its white petal variant (WR), were used to study the molecular mechanisms in flower color and scent. Sixty-five differential flavonoid metabolites and 15 volatiles were found to have significant differences between RR and WR. Correspondingly, the key regulators (MYB-bHLH-WD40) of anthocyanin synthesis pathway and their structural genes involved in flavonoid biosynthesis, benzenoid/phenylpropanoid biosynthesis, terpenoid biosynthesis pathways were also found to be differentially expressed by comparative transcriptome. Further, qPCR permitted the identification of some transcripts encoding proteins that were putatively associated with scent and color biosynthesis in roses. Particularly, the results showed that the ACT gene (encoding CoA geraniol/citronellol acetyltransferase, GeneID: 112190420), which expressed lower in WR, was involved in three pathways: flavonoid biosynthesis, phenylpropanoid biosynthesis and terpenoid biosynthesis, however, GT5 (anthocyanin glycosylation gene, GeneID:112186660), expressed higher in WR, was involved in both flavonoid and phenylpropanoid biosynthesis pathways. These results suggested that ACT and GT5 might play important roles in regulating the relationship of color pigmentation and volatile emission.https://www.maxapress.com/article/doi/10.48130/OPR-2021-0005rosa rugosaflavonoidanthocyaninscentphenylpropanoidterpenoid |
| spellingShingle | Dedang Feng Hao Zhang Xianqin Qiu Hongying Jian Qigang Wang Ningning Zhou Yu Ye Jun Lu Huijun Yan Kaixue Tang Comparative transcriptomic and metabonomic analysis revealed the relationships between biosynthesis of volatiles and flavonoid metabolites in Rosa rugosa Ornamental Plant Research rosa rugosa flavonoid anthocyanin scent phenylpropanoid terpenoid |
| title | Comparative transcriptomic and metabonomic analysis revealed the relationships between biosynthesis of volatiles and flavonoid metabolites in Rosa rugosa |
| title_full | Comparative transcriptomic and metabonomic analysis revealed the relationships between biosynthesis of volatiles and flavonoid metabolites in Rosa rugosa |
| title_fullStr | Comparative transcriptomic and metabonomic analysis revealed the relationships between biosynthesis of volatiles and flavonoid metabolites in Rosa rugosa |
| title_full_unstemmed | Comparative transcriptomic and metabonomic analysis revealed the relationships between biosynthesis of volatiles and flavonoid metabolites in Rosa rugosa |
| title_short | Comparative transcriptomic and metabonomic analysis revealed the relationships between biosynthesis of volatiles and flavonoid metabolites in Rosa rugosa |
| title_sort | comparative transcriptomic and metabonomic analysis revealed the relationships between biosynthesis of volatiles and flavonoid metabolites in rosa rugosa |
| topic | rosa rugosa flavonoid anthocyanin scent phenylpropanoid terpenoid |
| url | https://www.maxapress.com/article/doi/10.48130/OPR-2021-0005 |
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