An XMRV Derived Retroviral Vector as a Tool for Gene Transfer
<p>Abstract</p> <p>Background</p> <p>Retroviral vectors are widely used tools for gene delivery and gene therapy. They are useful for gene expression studies and genetic manipulation <it>in vitro </it>and <it>in vivo</it>. Many retroviral vectors...
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| Format: | Article |
| Language: | English |
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BMC
2011-06-01
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| Series: | Virology Journal |
| Subjects: | |
| Online Access: | http://www.virologyj.com/content/8/1/284 |
| _version_ | 1817993749427388416 |
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| author | Rojas-Martinez Augusto Cervantes-Garcia Daniel Camerini David |
| author_facet | Rojas-Martinez Augusto Cervantes-Garcia Daniel Camerini David |
| author_sort | Rojas-Martinez Augusto |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Retroviral vectors are widely used tools for gene delivery and gene therapy. They are useful for gene expression studies and genetic manipulation <it>in vitro </it>and <it>in vivo</it>. Many retroviral vectors are derived from the mouse gammaretrovirus, murine leukemia virus (MLV). These vectors have been widely used in gene therapy clinical trials. XMRV, initially found in prostate cancer tissue, was the first human gammaretrovirus described.</p> <p>Findings</p> <p>We developed a new retroviral vector based on XMRV called pXC. It was developed for gene transfer to human cells and is produced by transient cotransfection of LNCaP cells with pXC and XMRV-packaging plasmids.</p> <p>Conclusions</p> <p>We demonstrated that pXC mediates expression of inserted transgenes in cell lines. This new vector will be a useful tool for gene transfer in human and non-human cell lines, including gene therapy studies.</p> |
| first_indexed | 2024-04-14T01:43:00Z |
| format | Article |
| id | doaj.art-43e4b831ba36418699e0149964b5f77e |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-14T01:43:00Z |
| publishDate | 2011-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-43e4b831ba36418699e0149964b5f77e2022-12-22T02:19:38ZengBMCVirology Journal1743-422X2011-06-018128410.1186/1743-422X-8-284An XMRV Derived Retroviral Vector as a Tool for Gene TransferRojas-Martinez AugustoCervantes-Garcia DanielCamerini David<p>Abstract</p> <p>Background</p> <p>Retroviral vectors are widely used tools for gene delivery and gene therapy. They are useful for gene expression studies and genetic manipulation <it>in vitro </it>and <it>in vivo</it>. Many retroviral vectors are derived from the mouse gammaretrovirus, murine leukemia virus (MLV). These vectors have been widely used in gene therapy clinical trials. XMRV, initially found in prostate cancer tissue, was the first human gammaretrovirus described.</p> <p>Findings</p> <p>We developed a new retroviral vector based on XMRV called pXC. It was developed for gene transfer to human cells and is produced by transient cotransfection of LNCaP cells with pXC and XMRV-packaging plasmids.</p> <p>Conclusions</p> <p>We demonstrated that pXC mediates expression of inserted transgenes in cell lines. This new vector will be a useful tool for gene transfer in human and non-human cell lines, including gene therapy studies.</p>http://www.virologyj.com/content/8/1/284XMRVretroviral vectortransduction |
| spellingShingle | Rojas-Martinez Augusto Cervantes-Garcia Daniel Camerini David An XMRV Derived Retroviral Vector as a Tool for Gene Transfer Virology Journal XMRV retroviral vector transduction |
| title | An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
| title_full | An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
| title_fullStr | An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
| title_full_unstemmed | An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
| title_short | An XMRV Derived Retroviral Vector as a Tool for Gene Transfer |
| title_sort | xmrv derived retroviral vector as a tool for gene transfer |
| topic | XMRV retroviral vector transduction |
| url | http://www.virologyj.com/content/8/1/284 |
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