Usefulness of Molecular Methods for <i>Helicobacter pylori</i> Detection in Pediatric Patients and Their Correlation with Histopathological Sydney Classification
<i>Helicobacter pylori</i> infections, as one of the most prevalent among humans, are generally acquired during childhood, and are one of the main causes of chronic gastritis and peptic ulcer disease. A bacterial culture from a gastric biopsy is the gold standard and is the only method t...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/1/179 |
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| author | Tomasz Bogiel Agnieszka Mikucka Anna Szaflarska-Popławska Dariusz Grzanka |
| author_facet | Tomasz Bogiel Agnieszka Mikucka Anna Szaflarska-Popławska Dariusz Grzanka |
| author_sort | Tomasz Bogiel |
| collection | DOAJ |
| description | <i>Helicobacter pylori</i> infections, as one of the most prevalent among humans, are generally acquired during childhood, and are one of the main causes of chronic gastritis and peptic ulcer disease. A bacterial culture from a gastric biopsy is the gold standard and is the only method that has 100% specificity. However, its sensitivity varies, depending on experience of the laboratory staff, applied culture media, specimen transport conditions, biopsy site, and quality of the sample. The same factors compromise all invasive methods and a culture-based <i>H. pylori</i> infection diagnostic, as well as a recent intake of antibiotics, bismuth-containing compounds, and proton pump inhibitors. Molecular methods have been used for clinical microbiology investigation since the beginning of the 21st century. However, their usefulness for <i>H. pylori</i> infections diagnosis remains unclear, especially in pediatric patients. The aim of the study was to assess the incidence of <i>H. pylori</i> infections in a group of 104 pediatric patients and to compare the results of the PCR test with the corresponding histopathological investigation effects. Among the biopsy samples collected from 104 children, 44 (42.3%) were positive in PCR, while 43 (41.3%) and 39 (37.5%) presented histologically-confirmed signs of inflammation and <i>H. pylori</i> colonization, respectively. Moreover, the mean grades of the parameters of the histopathological examination were higher in the group of PCR-positive samples. The compatibility of both research methods was confirmed, emphasizing the usefulness of molecular methods for detecting <i>H. pylori</i> infections in pediatric patients. Considering that the PCR-based method gives reliable results and is less time-consuming and costly, it is worth discussing this method as a new standard in the diagnosis of <i>H. pylori</i> infections, at least among pediatric patients, for which culture-based diagnostics is not sufficient or histopathological examination is negative, while inflammation signs are observed macroscopically. |
| first_indexed | 2024-03-09T12:09:28Z |
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| language | English |
| last_indexed | 2024-03-09T12:09:28Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-43e5df1e1561416aadcff481298f08162023-11-30T22:54:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0124117910.3390/ijms24010179Usefulness of Molecular Methods for <i>Helicobacter pylori</i> Detection in Pediatric Patients and Their Correlation with Histopathological Sydney ClassificationTomasz Bogiel0Agnieszka Mikucka1Anna Szaflarska-Popławska2Dariusz Grzanka3Department of Microbiology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, 85-094 Bydgoszcz, PolandDepartment of Microbiology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, 85-094 Bydgoszcz, PolandDepartment of Pediatric Endoscopy and Gastrointestinal Function Testing, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, 85-094 Bydgoszcz, PolandDepartment of Clinical Pathomorphology, Faculty of Medicine, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland<i>Helicobacter pylori</i> infections, as one of the most prevalent among humans, are generally acquired during childhood, and are one of the main causes of chronic gastritis and peptic ulcer disease. A bacterial culture from a gastric biopsy is the gold standard and is the only method that has 100% specificity. However, its sensitivity varies, depending on experience of the laboratory staff, applied culture media, specimen transport conditions, biopsy site, and quality of the sample. The same factors compromise all invasive methods and a culture-based <i>H. pylori</i> infection diagnostic, as well as a recent intake of antibiotics, bismuth-containing compounds, and proton pump inhibitors. Molecular methods have been used for clinical microbiology investigation since the beginning of the 21st century. However, their usefulness for <i>H. pylori</i> infections diagnosis remains unclear, especially in pediatric patients. The aim of the study was to assess the incidence of <i>H. pylori</i> infections in a group of 104 pediatric patients and to compare the results of the PCR test with the corresponding histopathological investigation effects. Among the biopsy samples collected from 104 children, 44 (42.3%) were positive in PCR, while 43 (41.3%) and 39 (37.5%) presented histologically-confirmed signs of inflammation and <i>H. pylori</i> colonization, respectively. Moreover, the mean grades of the parameters of the histopathological examination were higher in the group of PCR-positive samples. The compatibility of both research methods was confirmed, emphasizing the usefulness of molecular methods for detecting <i>H. pylori</i> infections in pediatric patients. Considering that the PCR-based method gives reliable results and is less time-consuming and costly, it is worth discussing this method as a new standard in the diagnosis of <i>H. pylori</i> infections, at least among pediatric patients, for which culture-based diagnostics is not sufficient or histopathological examination is negative, while inflammation signs are observed macroscopically.https://www.mdpi.com/1422-0067/24/1/179<i>Helicobacter pylori</i>histopathological investigationmolecular diagnosticsPCRpediatric patientsreal-time PCR |
| spellingShingle | Tomasz Bogiel Agnieszka Mikucka Anna Szaflarska-Popławska Dariusz Grzanka Usefulness of Molecular Methods for <i>Helicobacter pylori</i> Detection in Pediatric Patients and Their Correlation with Histopathological Sydney Classification International Journal of Molecular Sciences <i>Helicobacter pylori</i> histopathological investigation molecular diagnostics PCR pediatric patients real-time PCR |
| title | Usefulness of Molecular Methods for <i>Helicobacter pylori</i> Detection in Pediatric Patients and Their Correlation with Histopathological Sydney Classification |
| title_full | Usefulness of Molecular Methods for <i>Helicobacter pylori</i> Detection in Pediatric Patients and Their Correlation with Histopathological Sydney Classification |
| title_fullStr | Usefulness of Molecular Methods for <i>Helicobacter pylori</i> Detection in Pediatric Patients and Their Correlation with Histopathological Sydney Classification |
| title_full_unstemmed | Usefulness of Molecular Methods for <i>Helicobacter pylori</i> Detection in Pediatric Patients and Their Correlation with Histopathological Sydney Classification |
| title_short | Usefulness of Molecular Methods for <i>Helicobacter pylori</i> Detection in Pediatric Patients and Their Correlation with Histopathological Sydney Classification |
| title_sort | usefulness of molecular methods for i helicobacter pylori i detection in pediatric patients and their correlation with histopathological sydney classification |
| topic | <i>Helicobacter pylori</i> histopathological investigation molecular diagnostics PCR pediatric patients real-time PCR |
| url | https://www.mdpi.com/1422-0067/24/1/179 |
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