Application of Topical Sucralfate and Topical Platelet-Rich Plasma Improves Wound Healing in Diabetic Ulcer Rats Wound Model
Renni Yuniati,1 Innelya Innelya,2 Arti Rachmawati,2 Harold Jefferson Matthew Charlex,2 Alfi Rahmatika,2 Matthew Brian Khrisna,1 Farmaditya EP Mundhofir,3 K Heri Nugroho Hario Seno,4 Tri Nur Kristina5 1Dermatology and Venereology Department, Faculty of Medicine, Diponegoro University, Semarang, Indon...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-08-01
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| Series: | Journal of Experimental Pharmacology |
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| Online Access: | https://www.dovepress.com/application-of-topical-sucralfate-and-topical-platelet-rich-plasma-imp-peer-reviewed-fulltext-article-JEP |
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| author | Yuniati R Innelya I Rachmawati A Charlex HJM Rahmatika A Khrisna MB Mundhofir FEP Hario Seno HNK Kristina TN |
| author_facet | Yuniati R Innelya I Rachmawati A Charlex HJM Rahmatika A Khrisna MB Mundhofir FEP Hario Seno HNK Kristina TN |
| author_sort | Yuniati R |
| collection | DOAJ |
| description | Renni Yuniati,1 Innelya Innelya,2 Arti Rachmawati,2 Harold Jefferson Matthew Charlex,2 Alfi Rahmatika,2 Matthew Brian Khrisna,1 Farmaditya EP Mundhofir,3 K Heri Nugroho Hario Seno,4 Tri Nur Kristina5 1Dermatology and Venereology Department, Faculty of Medicine, Diponegoro University, Semarang, Indonesia; 2Undergraduate Student, Faculty of Medicine, Diponegoro University, Semarang, Indonesia; 3Histology Department, Faculty of Medicine, Diponegoro University, Semarang, Indonesia; 4Internal Medicine Department, Faculty of Medicine, Diponegoro University, Semarang, Indonesia; 5Microbiology Department, Faculty of Medicine, Diponegoro University, Semarang, IndonesiaCorrespondence: Renni YuniatiDermatology and Venereology Department, Faculty of Medicine, Diponegoro University, Semarang, IndonesiaTel +628122874008Email renniyuniati@yahoo.comPurpose: One of the most serious and devastating complications of diabetes mellitus is diabetic ulcers. They are difficult to treat and often result in limb loss. Topical sucralfate and platelet-rich plasma have the potential to improve the healing outcomes of chronic ulcers, including diabetic ulcers. This research aims to determine the effectiveness of sucralfate and platelet-rich plasma therapy for the improvement of diabetic ulcer wound healing.Patients and Methods: Ninety Wistar rats were used in this study and were classified into five groups. Four of the five groups were diabetic induced and were treated with topical sucralfate only, platelet-rich plasma only, combination of topical sucralfate and platelet-rich plasma, and diabetic control group which received standard therapy only. The non-diabetic control group did not receive any therapy. We observed macrophage amount, platelet-derived growth factor, vascular endothelial growth factor, and hypoxia-inducible factor as a biomarker. Rats were terminated after 7th and 14th days and were subjected to immunohistochemistry staining and examination.Results: We found that topical sucralfate and platelet-rich plasma increase macrophage levels, vascular endothelial growth factor expression and platelet-derived growth factor expression in diabetic wound cells. We also found a reduction in hypoxia inducible factor-1α expression. Combination of topical sucralfate and platelet-rich plasma for 14 days gave the most significant improvement in terms of wound healing compared to topical sucralfate or platelet-rich plasma alone.Conclusion: The combination of topical sucralfate and platelet-rich plasma therapy results in the best improvement in diabetic ulcer wound healing compared to sucralfate or platelet-rich plasma monotherapy or conventional wound healing therapy.Keywords: hypoxia inducible factor, VEGF, PDGF, macrophage |
| first_indexed | 2024-12-21T06:19:59Z |
| format | Article |
| id | doaj.art-43e740bb82c04b58be3707e7095230ad |
| institution | Directory Open Access Journal |
| issn | 1179-1454 |
| language | English |
| last_indexed | 2024-12-21T06:19:59Z |
| publishDate | 2021-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Experimental Pharmacology |
| spelling | doaj.art-43e740bb82c04b58be3707e7095230ad2022-12-21T19:13:18ZengDove Medical PressJournal of Experimental Pharmacology1179-14542021-08-01Volume 1379780667893Application of Topical Sucralfate and Topical Platelet-Rich Plasma Improves Wound Healing in Diabetic Ulcer Rats Wound ModelYuniati RInnelya IRachmawati ACharlex HJMRahmatika AKhrisna MBMundhofir FEPHario Seno HNKKristina TNRenni Yuniati,1 Innelya Innelya,2 Arti Rachmawati,2 Harold Jefferson Matthew Charlex,2 Alfi Rahmatika,2 Matthew Brian Khrisna,1 Farmaditya EP Mundhofir,3 K Heri Nugroho Hario Seno,4 Tri Nur Kristina5 1Dermatology and Venereology Department, Faculty of Medicine, Diponegoro University, Semarang, Indonesia; 2Undergraduate Student, Faculty of Medicine, Diponegoro University, Semarang, Indonesia; 3Histology Department, Faculty of Medicine, Diponegoro University, Semarang, Indonesia; 4Internal Medicine Department, Faculty of Medicine, Diponegoro University, Semarang, Indonesia; 5Microbiology Department, Faculty of Medicine, Diponegoro University, Semarang, IndonesiaCorrespondence: Renni YuniatiDermatology and Venereology Department, Faculty of Medicine, Diponegoro University, Semarang, IndonesiaTel +628122874008Email renniyuniati@yahoo.comPurpose: One of the most serious and devastating complications of diabetes mellitus is diabetic ulcers. They are difficult to treat and often result in limb loss. Topical sucralfate and platelet-rich plasma have the potential to improve the healing outcomes of chronic ulcers, including diabetic ulcers. This research aims to determine the effectiveness of sucralfate and platelet-rich plasma therapy for the improvement of diabetic ulcer wound healing.Patients and Methods: Ninety Wistar rats were used in this study and were classified into five groups. Four of the five groups were diabetic induced and were treated with topical sucralfate only, platelet-rich plasma only, combination of topical sucralfate and platelet-rich plasma, and diabetic control group which received standard therapy only. The non-diabetic control group did not receive any therapy. We observed macrophage amount, platelet-derived growth factor, vascular endothelial growth factor, and hypoxia-inducible factor as a biomarker. Rats were terminated after 7th and 14th days and were subjected to immunohistochemistry staining and examination.Results: We found that topical sucralfate and platelet-rich plasma increase macrophage levels, vascular endothelial growth factor expression and platelet-derived growth factor expression in diabetic wound cells. We also found a reduction in hypoxia inducible factor-1α expression. Combination of topical sucralfate and platelet-rich plasma for 14 days gave the most significant improvement in terms of wound healing compared to topical sucralfate or platelet-rich plasma alone.Conclusion: The combination of topical sucralfate and platelet-rich plasma therapy results in the best improvement in diabetic ulcer wound healing compared to sucralfate or platelet-rich plasma monotherapy or conventional wound healing therapy.Keywords: hypoxia inducible factor, VEGF, PDGF, macrophagehttps://www.dovepress.com/application-of-topical-sucralfate-and-topical-platelet-rich-plasma-imp-peer-reviewed-fulltext-article-JEPhypoxia inducive factorvegfpdgfmacrophage |
| spellingShingle | Yuniati R Innelya I Rachmawati A Charlex HJM Rahmatika A Khrisna MB Mundhofir FEP Hario Seno HNK Kristina TN Application of Topical Sucralfate and Topical Platelet-Rich Plasma Improves Wound Healing in Diabetic Ulcer Rats Wound Model Journal of Experimental Pharmacology hypoxia inducive factor vegf pdgf macrophage |
| title | Application of Topical Sucralfate and Topical Platelet-Rich Plasma Improves Wound Healing in Diabetic Ulcer Rats Wound Model |
| title_full | Application of Topical Sucralfate and Topical Platelet-Rich Plasma Improves Wound Healing in Diabetic Ulcer Rats Wound Model |
| title_fullStr | Application of Topical Sucralfate and Topical Platelet-Rich Plasma Improves Wound Healing in Diabetic Ulcer Rats Wound Model |
| title_full_unstemmed | Application of Topical Sucralfate and Topical Platelet-Rich Plasma Improves Wound Healing in Diabetic Ulcer Rats Wound Model |
| title_short | Application of Topical Sucralfate and Topical Platelet-Rich Plasma Improves Wound Healing in Diabetic Ulcer Rats Wound Model |
| title_sort | application of topical sucralfate and topical platelet rich plasma improves wound healing in diabetic ulcer rats wound model |
| topic | hypoxia inducive factor vegf pdgf macrophage |
| url | https://www.dovepress.com/application-of-topical-sucralfate-and-topical-platelet-rich-plasma-imp-peer-reviewed-fulltext-article-JEP |
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