Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors
Alkanethiol self-assembled monolayers (SAMs) with Peptide Nucleic Acid (PNA) were proposed as a novel probe design in electrochemical biosensors. This paper investigates the optimization of PNA probe by using two different concentrations of SAMs, immobilization temperature, and two-stage immobilizat...
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| Format: | Article |
| Language: | English |
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Düzce University
2019-07-01
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| Series: | Düzce Üniversitesi Bilim ve Teknoloji Dergisi |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/771236 |
| Summary: | Alkanethiol
self-assembled monolayers (SAMs) with Peptide Nucleic Acid (PNA) were proposed
as a novel probe design in electrochemical biosensors. This paper investigates
the optimization of PNA probe by using two different concentrations of SAMs,
immobilization temperature, and two-stage immobilization process. Alkanethiol
self-assembled monolayers with PNA probe immobilization and cDNA (complementary
DNA) hybridization were measured via AC voltammetry method. The small negative
shift in formal potential (-17 mV) was occurred after cDNA hybridization at
high ionic strength concentration. |
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| ISSN: | 2148-2446 |