Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors
Alkanethiolself-assembled monolayers (SAMs) with Peptide Nucleic Acid (PNA) were proposedas a novel probe design in electrochemical biosensors. This paper investigatesthe optimization of PNA probe by using two different concentrations of SAMs,immobilization temperature, and two-stage immobilization...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Düzce University
2019-07-01
|
| Series: | Düzce Üniversitesi Bilim ve Teknoloji Dergisi |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/download/article-file/771236 |
| _version_ | 1826544803702112256 |
|---|---|
| author | Zehra Banu Bahsi Oral |
| author_facet | Zehra Banu Bahsi Oral |
| author_sort | Zehra Banu Bahsi Oral |
| collection | DOAJ |
| description | Alkanethiolself-assembled monolayers (SAMs) with Peptide Nucleic Acid (PNA) were proposedas a novel probe design in electrochemical biosensors. This paper investigatesthe optimization of PNA probe by using two different concentrations of SAMs,immobilization temperature, and two-stage immobilization process. Alkanethiolself-assembled monolayers with PNA probe immobilization and cDNA (complementaryDNA) hybridization were measured via AC voltammetry method. The small negativeshift in formal potential (-17 mV) was occurred after cDNA hybridization athigh ionic strength concentration. |
| first_indexed | 2024-03-07T23:11:53Z |
| format | Article |
| id | doaj.art-43e7d256b7b54083bf318f113a93713c |
| institution | Directory Open Access Journal |
| issn | 2148-2446 |
| language | English |
| last_indexed | 2025-03-14T05:07:47Z |
| publishDate | 2019-07-01 |
| publisher | Düzce University |
| record_format | Article |
| series | Düzce Üniversitesi Bilim ve Teknoloji Dergisi |
| spelling | doaj.art-43e7d256b7b54083bf318f113a93713c2025-03-06T18:57:23ZengDüzce UniversityDüzce Üniversitesi Bilim ve Teknoloji Dergisi2148-24462019-07-01731806181210.29130/dubited.55815597Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical BiosensorsZehra Banu Bahsi Oral0https://orcid.org/0000-0001-5513-0847GEBZE TEKNİK ÜNİVERSİTESİ, BİYOTEKNOLOJİ ENSTİTÜSÜAlkanethiolself-assembled monolayers (SAMs) with Peptide Nucleic Acid (PNA) were proposedas a novel probe design in electrochemical biosensors. This paper investigatesthe optimization of PNA probe by using two different concentrations of SAMs,immobilization temperature, and two-stage immobilization process. Alkanethiolself-assembled monolayers with PNA probe immobilization and cDNA (complementaryDNA) hybridization were measured via AC voltammetry method. The small negativeshift in formal potential (-17 mV) was occurred after cDNA hybridization athigh ionic strength concentration.https://dergipark.org.tr/tr/download/article-file/771236alkanethiol self-assembled monolayerspnaelectrochemical biosensorsac voltammetryalkanetiol kendiliğinden oluşan tek katmanlarpnaelektrokimyasal biyosensörlerac voltametre |
| spellingShingle | Zehra Banu Bahsi Oral Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors Düzce Üniversitesi Bilim ve Teknoloji Dergisi alkanethiol self-assembled monolayers pna electrochemical biosensors ac voltammetry alkanetiol kendiliğinden oluşan tek katmanlar pna elektrokimyasal biyosensörler ac voltametre |
| title | Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors |
| title_full | Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors |
| title_fullStr | Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors |
| title_full_unstemmed | Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors |
| title_short | Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors |
| title_sort | self assembled monolayers with peptide nucleic acid pna probe to develop electrochemical biosensors |
| topic | alkanethiol self-assembled monolayers pna electrochemical biosensors ac voltammetry alkanetiol kendiliğinden oluşan tek katmanlar pna elektrokimyasal biyosensörler ac voltametre |
| url | https://dergipark.org.tr/tr/download/article-file/771236 |
| work_keys_str_mv | AT zehrabanubahsioral selfassembledmonolayerswithpeptidenucleicacidpnaprobetodevelopelectrochemicalbiosensors |