Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors
Alkanethiol self-assembled monolayers (SAMs) with Peptide Nucleic Acid (PNA) were proposed as a novel probe design in electrochemical biosensors. This paper investigates the optimization of PNA probe by using two different concentrations of SAMs, immobilization temperature, and two-stage immobilizat...
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| Format: | Article |
| Language: | English |
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Düzce University
2019-07-01
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| Series: | Düzce Üniversitesi Bilim ve Teknoloji Dergisi |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/771236 |
| _version_ | 1797300698181795840 |
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| author | Zehra Banu Bahsi Oral |
| author_facet | Zehra Banu Bahsi Oral |
| author_sort | Zehra Banu Bahsi Oral |
| collection | DOAJ |
| description | Alkanethiol
self-assembled monolayers (SAMs) with Peptide Nucleic Acid (PNA) were proposed
as a novel probe design in electrochemical biosensors. This paper investigates
the optimization of PNA probe by using two different concentrations of SAMs,
immobilization temperature, and two-stage immobilization process. Alkanethiol
self-assembled monolayers with PNA probe immobilization and cDNA (complementary
DNA) hybridization were measured via AC voltammetry method. The small negative
shift in formal potential (-17 mV) was occurred after cDNA hybridization at
high ionic strength concentration. |
| first_indexed | 2024-03-07T23:11:53Z |
| format | Article |
| id | doaj.art-43e7d256b7b54083bf318f113a93713c |
| institution | Directory Open Access Journal |
| issn | 2148-2446 |
| language | English |
| last_indexed | 2024-03-07T23:11:53Z |
| publishDate | 2019-07-01 |
| publisher | Düzce University |
| record_format | Article |
| series | Düzce Üniversitesi Bilim ve Teknoloji Dergisi |
| spelling | doaj.art-43e7d256b7b54083bf318f113a93713c2024-02-21T14:07:35ZengDüzce UniversityDüzce Üniversitesi Bilim ve Teknoloji Dergisi2148-24462019-07-01731806181210.29130/dubited.55815597Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical BiosensorsZehra Banu Bahsi Oral0GEBZE TEKNİK ÜNİVERSİTESİ, BİYOTEKNOLOJİ ENSTİTÜSÜAlkanethiol self-assembled monolayers (SAMs) with Peptide Nucleic Acid (PNA) were proposed as a novel probe design in electrochemical biosensors. This paper investigates the optimization of PNA probe by using two different concentrations of SAMs, immobilization temperature, and two-stage immobilization process. Alkanethiol self-assembled monolayers with PNA probe immobilization and cDNA (complementary DNA) hybridization were measured via AC voltammetry method. The small negative shift in formal potential (-17 mV) was occurred after cDNA hybridization at high ionic strength concentration.https://dergipark.org.tr/tr/download/article-file/771236alkanethiol self-assembled monolayerspnaelectrochemical biosensorsac voltammetryalkanetiol kendiliğinden oluşan tek katmanlarpnaelektrokimyasal biyosensörlerac voltametre |
| spellingShingle | Zehra Banu Bahsi Oral Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors Düzce Üniversitesi Bilim ve Teknoloji Dergisi alkanethiol self-assembled monolayers pna electrochemical biosensors ac voltammetry alkanetiol kendiliğinden oluşan tek katmanlar pna elektrokimyasal biyosensörler ac voltametre |
| title | Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors |
| title_full | Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors |
| title_fullStr | Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors |
| title_full_unstemmed | Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors |
| title_short | Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors |
| title_sort | self assembled monolayers with peptide nucleic acid pna probe to develop electrochemical biosensors |
| topic | alkanethiol self-assembled monolayers pna electrochemical biosensors ac voltammetry alkanetiol kendiliğinden oluşan tek katmanlar pna elektrokimyasal biyosensörler ac voltametre |
| url | https://dergipark.org.tr/tr/download/article-file/771236 |
| work_keys_str_mv | AT zehrabanubahsioral selfassembledmonolayerswithpeptidenucleicacidpnaprobetodevelopelectrochemicalbiosensors |