Mycobacterium tuberculosis antigens MPT63 and MPT83 increase phagocytic activity of murine peritoneal macrophages
Macrophages (MΦ) are the most described and characterized target and host of mycobacteria. Like other cells of innate immunity MΦ have a wide range of receptor molecules which interact with different pathogen associated molecular patterns (PAMPs). Immunodominant proteins MPT63 and MPT83 that are syn...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2016-08-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2016/11/Siromolot_5_16.pdf |
| _version_ | 1797668830064934912 |
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| author | A. A. Siromolot O. S. Oliinyk D. V. Kolibo S. V. Komisarenko |
| author_facet | A. A. Siromolot O. S. Oliinyk D. V. Kolibo S. V. Komisarenko |
| author_sort | A. A. Siromolot |
| collection | DOAJ |
| description | Macrophages (MΦ) are the most described and characterized target and host of mycobacteria. Like other cells of innate immunity MΦ have a wide range of receptor molecules which interact with different pathogen associated molecular patterns (PAMPs). Immunodominant proteins MPT63 and MPT83 that are synthesized in abundance by Mycobacterium bovis or Mycobacterium tuberculosis strains could be involved in development of tuberculosis infection. The aim of this study was to search for effects of these mycobacterial antigens on target cells. For this aim full-sized sequences of MPT83 (rMPT83full) and MPT63 antigens were cloned into plasmid pET24a(+). The increase of phagocytic activity of murine peritoneal macrophages was demonstrated, but not of macrophage-like cells from J774 cell line, which were treated by rMPT63 and rMPT83full proteins for 24 h. This effect of such antigens can be considered as a way to facilitate the consumption of mycobacterial cells by macrophages to avoid other effector mechanisms of innate and adaptive immunity. |
| first_indexed | 2024-03-11T20:34:11Z |
| format | Article |
| id | doaj.art-43e8a83f6b7c4b39be9df15cab742802 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2409-4943 |
| language | English |
| last_indexed | 2024-03-11T20:34:11Z |
| publishDate | 2016-08-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-43e8a83f6b7c4b39be9df15cab7428022023-10-02T07:19:12ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432409-49432016-08-01885627010.15407/ubj88.05.062Mycobacterium tuberculosis antigens MPT63 and MPT83 increase phagocytic activity of murine peritoneal macrophagesA. A. Siromolot0 O. S. Oliinyk1 D. V. Kolibo 2S. V. Komisarenko3Educational and Scientific Centre Institute of Biology, Taras Shevchenko National University of Kyiv, Ukraine;Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv 2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivEducational and Scientific Centre Institute of Biology, Taras Shevchenko National University of Kyiv, Ukraine; Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyive; 2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivMacrophages (MΦ) are the most described and characterized target and host of mycobacteria. Like other cells of innate immunity MΦ have a wide range of receptor molecules which interact with different pathogen associated molecular patterns (PAMPs). Immunodominant proteins MPT63 and MPT83 that are synthesized in abundance by Mycobacterium bovis or Mycobacterium tuberculosis strains could be involved in development of tuberculosis infection. The aim of this study was to search for effects of these mycobacterial antigens on target cells. For this aim full-sized sequences of MPT83 (rMPT83full) and MPT63 antigens were cloned into plasmid pET24a(+). The increase of phagocytic activity of murine peritoneal macrophages was demonstrated, but not of macrophage-like cells from J774 cell line, which were treated by rMPT63 and rMPT83full proteins for 24 h. This effect of such antigens can be considered as a way to facilitate the consumption of mycobacterial cells by macrophages to avoid other effector mechanisms of innate and adaptive immunity.http://ukrbiochemjournal.org/wp-content/uploads/2016/11/Siromolot_5_16.pdfmacrophagesMPT63MPT83Mycobacterium bovisMycobacterium tuberculosisphagocytosis |
| spellingShingle | A. A. Siromolot O. S. Oliinyk D. V. Kolibo S. V. Komisarenko Mycobacterium tuberculosis antigens MPT63 and MPT83 increase phagocytic activity of murine peritoneal macrophages The Ukrainian Biochemical Journal macrophages MPT63 MPT83 Mycobacterium bovis Mycobacterium tuberculosis phagocytosis |
| title | Mycobacterium tuberculosis antigens MPT63 and MPT83 increase phagocytic activity of murine peritoneal macrophages |
| title_full | Mycobacterium tuberculosis antigens MPT63 and MPT83 increase phagocytic activity of murine peritoneal macrophages |
| title_fullStr | Mycobacterium tuberculosis antigens MPT63 and MPT83 increase phagocytic activity of murine peritoneal macrophages |
| title_full_unstemmed | Mycobacterium tuberculosis antigens MPT63 and MPT83 increase phagocytic activity of murine peritoneal macrophages |
| title_short | Mycobacterium tuberculosis antigens MPT63 and MPT83 increase phagocytic activity of murine peritoneal macrophages |
| title_sort | mycobacterium tuberculosis antigens mpt63 and mpt83 increase phagocytic activity of murine peritoneal macrophages |
| topic | macrophages MPT63 MPT83 Mycobacterium bovis Mycobacterium tuberculosis phagocytosis |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2016/11/Siromolot_5_16.pdf |
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