Charge-specific adverse effects of polystyrene nanoplastics on zebrafish (Danio rerio) development and behavior
Nanoplastics are being detected with increasing frequency in aquatic environments. Although evidence suggests that nanoplastics can cause overt toxicity to biota across different trophic levels, but there is little understanding of how materials such as differently charged polystyrene nanoplastics (...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Environment International |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412022000800 |
| _version_ | 1818060427151540224 |
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| author | Miaomiao Teng Xiaoli Zhao Fengchang Wu Chengju Wang Chen Wang Jason C. White Wentian Zhao Lingfeng Zhou Sen Yan Sinuo Tian |
| author_facet | Miaomiao Teng Xiaoli Zhao Fengchang Wu Chengju Wang Chen Wang Jason C. White Wentian Zhao Lingfeng Zhou Sen Yan Sinuo Tian |
| author_sort | Miaomiao Teng |
| collection | DOAJ |
| description | Nanoplastics are being detected with increasing frequency in aquatic environments. Although evidence suggests that nanoplastics can cause overt toxicity to biota across different trophic levels, but there is little understanding of how materials such as differently charged polystyrene nanoplastics (PS-NP) impact fish development and behavior. Following exposure to amino-modified (positive charge) PS-NP, fluorescence accumulation was observed in the zebrafish brain and gastrointestinal tract. Positively charged PS-NP induced stronger developmental toxicity (decreased spontaneous movement, heartbeat, hatching rate, and length) and cell apoptosis in the brain and induced greater neurobehavioral impairment as compared to carboxyl-modified (negative charge) PS-NP. These findings correlated well with fluorescence differences indicating PS-NP presence. Targeted neuro-metabolite analysis by UHPLC-MS/MS reveals that positively charged PS-NP decreased levels of glycine, cysteine, glutathione, and glutamic acid, while the increased levels of spermine, spermidine, and tyramine were induced by negatively charged PS-NP. Positively charged PS-NP interacted with the neurotransmitter receptor N-methyl-D-aspartate receptor 2B (NMDA2B), whereas negatively charged PS-NP impacted the G-protein-coupled receptor 1 (GPR1), each with different binding energies that led to behavioral differences. These findings reveal the charge-specific toxicity of nanoplastics to fish and provide new perspective for understanding PS-NP neurotoxicity that is needed to accurately assess potential environmental and health risks of these emerging contaminants. |
| first_indexed | 2024-12-10T13:32:15Z |
| format | Article |
| id | doaj.art-43ea7ea1c998428caaeec889ae24f132 |
| institution | Directory Open Access Journal |
| issn | 0160-4120 |
| language | English |
| last_indexed | 2024-12-10T13:32:15Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environment International |
| spelling | doaj.art-43ea7ea1c998428caaeec889ae24f1322022-12-22T01:46:55ZengElsevierEnvironment International0160-41202022-05-01163107154Charge-specific adverse effects of polystyrene nanoplastics on zebrafish (Danio rerio) development and behaviorMiaomiao Teng0Xiaoli Zhao1Fengchang Wu2Chengju Wang3Chen Wang4Jason C. White5Wentian Zhao6Lingfeng Zhou7Sen Yan8Sinuo Tian9State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, ChinaState Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; Corresponding authors at: Anwai Dayang Fang 8#, Chaoyang District, Beijing, China.State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; Corresponding authors at: Anwai Dayang Fang 8#, Chaoyang District, Beijing, China.Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, ChinaThe Connecticut Agricultural Experiment Station, New Haven, Connecticut 06511, USAInnovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, ChinaInnovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, ChinaInnovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, ChinaNanoplastics are being detected with increasing frequency in aquatic environments. Although evidence suggests that nanoplastics can cause overt toxicity to biota across different trophic levels, but there is little understanding of how materials such as differently charged polystyrene nanoplastics (PS-NP) impact fish development and behavior. Following exposure to amino-modified (positive charge) PS-NP, fluorescence accumulation was observed in the zebrafish brain and gastrointestinal tract. Positively charged PS-NP induced stronger developmental toxicity (decreased spontaneous movement, heartbeat, hatching rate, and length) and cell apoptosis in the brain and induced greater neurobehavioral impairment as compared to carboxyl-modified (negative charge) PS-NP. These findings correlated well with fluorescence differences indicating PS-NP presence. Targeted neuro-metabolite analysis by UHPLC-MS/MS reveals that positively charged PS-NP decreased levels of glycine, cysteine, glutathione, and glutamic acid, while the increased levels of spermine, spermidine, and tyramine were induced by negatively charged PS-NP. Positively charged PS-NP interacted with the neurotransmitter receptor N-methyl-D-aspartate receptor 2B (NMDA2B), whereas negatively charged PS-NP impacted the G-protein-coupled receptor 1 (GPR1), each with different binding energies that led to behavioral differences. These findings reveal the charge-specific toxicity of nanoplastics to fish and provide new perspective for understanding PS-NP neurotoxicity that is needed to accurately assess potential environmental and health risks of these emerging contaminants.http://www.sciencedirect.com/science/article/pii/S0160412022000800Differentially chargedPolystyrene nanoplasticsZebrafishBehavioral activityNeurotransmitters |
| spellingShingle | Miaomiao Teng Xiaoli Zhao Fengchang Wu Chengju Wang Chen Wang Jason C. White Wentian Zhao Lingfeng Zhou Sen Yan Sinuo Tian Charge-specific adverse effects of polystyrene nanoplastics on zebrafish (Danio rerio) development and behavior Environment International Differentially charged Polystyrene nanoplastics Zebrafish Behavioral activity Neurotransmitters |
| title | Charge-specific adverse effects of polystyrene nanoplastics on zebrafish (Danio rerio) development and behavior |
| title_full | Charge-specific adverse effects of polystyrene nanoplastics on zebrafish (Danio rerio) development and behavior |
| title_fullStr | Charge-specific adverse effects of polystyrene nanoplastics on zebrafish (Danio rerio) development and behavior |
| title_full_unstemmed | Charge-specific adverse effects of polystyrene nanoplastics on zebrafish (Danio rerio) development and behavior |
| title_short | Charge-specific adverse effects of polystyrene nanoplastics on zebrafish (Danio rerio) development and behavior |
| title_sort | charge specific adverse effects of polystyrene nanoplastics on zebrafish danio rerio development and behavior |
| topic | Differentially charged Polystyrene nanoplastics Zebrafish Behavioral activity Neurotransmitters |
| url | http://www.sciencedirect.com/science/article/pii/S0160412022000800 |
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