Development and validation of a LC-ESI-MS/MS method for simultaneous whole blood analysis of 51 new psychoactive substances
In recent years, there has been a great increase in seizures and forensic analysis of new psychotropic substances (NPS) in the state of Rio Grande do Sul. The analysis of these compounds needs to be performed in biological samples in cases of violent deaths. A sensitive and reliable liquid chromato...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal do Rio Grande do Sul
2019-12-01
|
| Series: | Drug Analytical Research |
| Online Access: | https://seer.ufrgs.br/index.php/poled/management/settings/website/index.php/dar/article/view/97423 |
| _version_ | 1797318040676728832 |
|---|---|
| author | Maria Cristina Franck Maristela Goldnadel Monteiro Renata Pereira Limberger |
| author_facet | Maria Cristina Franck Maristela Goldnadel Monteiro Renata Pereira Limberger |
| author_sort | Maria Cristina Franck |
| collection | DOAJ |
| description |
In recent years, there has been a great increase in seizures and forensic analysis of new psychotropic substances (NPS) in the state of Rio Grande do Sul. The analysis of these compounds needs to be performed in biological samples in cases of violent deaths. A sensitive and reliable liquid chromatography-tandem mass spectrometry with electrospray ionization interface (LC-ESI-MS/MS) method was developed and validated for qualitative analysis of 51 NPS in whole blood forensic samples. Synthetic cathinones, phenethylamines, opioids, tryptamines, synthetic cannabinoids, and other hallucinogens and stimulants were included in the method. The validation parameters assessed were specificity, limit of detection, retention time precision, and matrix effect. Drug free pools (n=6) were used for validation, including post mortem samples as well as from living individuals. Adulterants, pharmaceuticals, metabolites, and other illicit drugs, totalling 39 compounds, were analyzed and no interference was noticed. The detection limits obtained were suitable for evaluation at recreational and non-fatal levels of consumption, mostly. The results revealed an appropriate matrix effect in 24 out of 51 substances tested, indicating the potential for future quantitative analysis with this method for these drugs. The developed and validated method is easy to implement, fast, with low cost, and suitable for use in routine forensic toxicology laboratory analysis.
|
| first_indexed | 2024-03-08T03:44:42Z |
| format | Article |
| id | doaj.art-43eebe1557f54718b24f266e2f674878 |
| institution | Directory Open Access Journal |
| issn | 2527-2616 |
| language | English |
| last_indexed | 2024-03-08T03:44:42Z |
| publishDate | 2019-12-01 |
| publisher | Universidade Federal do Rio Grande do Sul |
| record_format | Article |
| series | Drug Analytical Research |
| spelling | doaj.art-43eebe1557f54718b24f266e2f6748782024-02-09T18:14:46ZengUniversidade Federal do Rio Grande do SulDrug Analytical Research2527-26162019-12-0132Development and validation of a LC-ESI-MS/MS method for simultaneous whole blood analysis of 51 new psychoactive substancesMaria Cristina Franck0Maristela Goldnadel Monteiro1Renata Pereira Limberger2Instituto-Geral de Perícias do RS PPGCF da UFRGSPan American Health OrganizationPPGCF da UFRGS In recent years, there has been a great increase in seizures and forensic analysis of new psychotropic substances (NPS) in the state of Rio Grande do Sul. The analysis of these compounds needs to be performed in biological samples in cases of violent deaths. A sensitive and reliable liquid chromatography-tandem mass spectrometry with electrospray ionization interface (LC-ESI-MS/MS) method was developed and validated for qualitative analysis of 51 NPS in whole blood forensic samples. Synthetic cathinones, phenethylamines, opioids, tryptamines, synthetic cannabinoids, and other hallucinogens and stimulants were included in the method. The validation parameters assessed were specificity, limit of detection, retention time precision, and matrix effect. Drug free pools (n=6) were used for validation, including post mortem samples as well as from living individuals. Adulterants, pharmaceuticals, metabolites, and other illicit drugs, totalling 39 compounds, were analyzed and no interference was noticed. The detection limits obtained were suitable for evaluation at recreational and non-fatal levels of consumption, mostly. The results revealed an appropriate matrix effect in 24 out of 51 substances tested, indicating the potential for future quantitative analysis with this method for these drugs. The developed and validated method is easy to implement, fast, with low cost, and suitable for use in routine forensic toxicology laboratory analysis. https://seer.ufrgs.br/index.php/poled/management/settings/website/index.php/dar/article/view/97423 |
| spellingShingle | Maria Cristina Franck Maristela Goldnadel Monteiro Renata Pereira Limberger Development and validation of a LC-ESI-MS/MS method for simultaneous whole blood analysis of 51 new psychoactive substances Drug Analytical Research |
| title | Development and validation of a LC-ESI-MS/MS method for simultaneous whole blood analysis of 51 new psychoactive substances |
| title_full | Development and validation of a LC-ESI-MS/MS method for simultaneous whole blood analysis of 51 new psychoactive substances |
| title_fullStr | Development and validation of a LC-ESI-MS/MS method for simultaneous whole blood analysis of 51 new psychoactive substances |
| title_full_unstemmed | Development and validation of a LC-ESI-MS/MS method for simultaneous whole blood analysis of 51 new psychoactive substances |
| title_short | Development and validation of a LC-ESI-MS/MS method for simultaneous whole blood analysis of 51 new psychoactive substances |
| title_sort | development and validation of a lc esi ms ms method for simultaneous whole blood analysis of 51 new psychoactive substances |
| url | https://seer.ufrgs.br/index.php/poled/management/settings/website/index.php/dar/article/view/97423 |
| work_keys_str_mv | AT mariacristinafranck developmentandvalidationofalcesimsmsmethodforsimultaneouswholebloodanalysisof51newpsychoactivesubstances AT maristelagoldnadelmonteiro developmentandvalidationofalcesimsmsmethodforsimultaneouswholebloodanalysisof51newpsychoactivesubstances AT renatapereiralimberger developmentandvalidationofalcesimsmsmethodforsimultaneouswholebloodanalysisof51newpsychoactivesubstances |