Evodiamine inhibits EPRS expression to regulate glutamate metabolism and proliferation of oral squamous cell carcinoma cells
Abstract The effects of evodiamine (EVO) on oral squamous cell carcinoma (OSCC) are not yet understood. Based on our earlier findings, we hypothesized that evodiamine may affect OSCC cell proliferation and glutamate metabolism by modulating the expression of EPRS (glutamyl‐prolyl‐tRNA synthetase 1)....
| প্রধান লেখক: | , , , , , , |
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| বিন্যাস: | প্রবন্ধ |
| ভাষা: | English |
| প্রকাশিত: |
Wiley
2024-04-01
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| মালা: | Kaohsiung Journal of Medical Sciences |
| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://doi.org/10.1002/kjm2.12803 |
| _version_ | 1827289684659666944 |
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| author | Li‐Qi Lin Si‐Yi Lv Hao‐Zhe Ren Rong‐Rong Li Lin Li Yun‐Qing Pang Jing Wang |
| author_facet | Li‐Qi Lin Si‐Yi Lv Hao‐Zhe Ren Rong‐Rong Li Lin Li Yun‐Qing Pang Jing Wang |
| author_sort | Li‐Qi Lin |
| collection | DOAJ |
| description | Abstract The effects of evodiamine (EVO) on oral squamous cell carcinoma (OSCC) are not yet understood. Based on our earlier findings, we hypothesized that evodiamine may affect OSCC cell proliferation and glutamate metabolism by modulating the expression of EPRS (glutamyl‐prolyl‐tRNA synthetase 1). From GEPIA, we obtained EPRS expression data in patients with OSCC as well as survival prognosis data. An animal model using Cal27 cells in BALB/c nude mice was established. The expression of EPRS was assessed by immunofluorescence, Western blotting, and quantitative PCR. Glutamate measurements were performed to evaluate the impact of evodiamine on glutamate metabolism of Cal27 and SAS tumor cells. transient transfection techniques were used to knock down and modulate EPRS in these cells. EPRS is expressed at higher levels in OSCC than in normal tissues, and it predicts poor prognosis in patients. In a nude mouse xenograft model, evodiamine inhibited tumor growth and the expression of EPRS. Evodiamine impacted cell proliferation, glutamine metabolism, and EPRS expression on Cal27 and SAS cell lines. In EPRS knockdown cell lines, both cell proliferation and glutamine metabolism are suppressed. EPRS's overexpression partially restores evodiamine's inhibitory effects on cell proliferation and glutamine metabolism. This study provides crucial experimental evidence supporting the potential therapeutic application of evodiamine in treating OSCC. Evodiamine exhibits promising anti‐tumor effects by targeting EPRS to regulate glutamate metabolism. |
| first_indexed | 2024-04-24T11:54:17Z |
| format | Article |
| id | doaj.art-43f14f31f876482ea425f7d4bc504af7 |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-04-24T11:54:17Z |
| publishDate | 2024-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-43f14f31f876482ea425f7d4bc504af72024-04-09T05:56:38ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502024-04-0140434835910.1002/kjm2.12803Evodiamine inhibits EPRS expression to regulate glutamate metabolism and proliferation of oral squamous cell carcinoma cellsLi‐Qi Lin0Si‐Yi Lv1Hao‐Zhe Ren2Rong‐Rong Li3Lin Li4Yun‐Qing Pang5Jing Wang6School/Hospital of Stomatology Lanzhou University Lanzhou Gansu ChinaSchool/Hospital of Stomatology Lanzhou University Lanzhou Gansu ChinaSchool/Hospital of Stomatology Lanzhou University Lanzhou Gansu ChinaSchool/Hospital of Stomatology Lanzhou University Lanzhou Gansu ChinaSchool/Hospital of Stomatology Lanzhou University Lanzhou Gansu ChinaSchool/Hospital of Stomatology Lanzhou University Lanzhou Gansu ChinaSchool/Hospital of Stomatology Lanzhou University Lanzhou Gansu ChinaAbstract The effects of evodiamine (EVO) on oral squamous cell carcinoma (OSCC) are not yet understood. Based on our earlier findings, we hypothesized that evodiamine may affect OSCC cell proliferation and glutamate metabolism by modulating the expression of EPRS (glutamyl‐prolyl‐tRNA synthetase 1). From GEPIA, we obtained EPRS expression data in patients with OSCC as well as survival prognosis data. An animal model using Cal27 cells in BALB/c nude mice was established. The expression of EPRS was assessed by immunofluorescence, Western blotting, and quantitative PCR. Glutamate measurements were performed to evaluate the impact of evodiamine on glutamate metabolism of Cal27 and SAS tumor cells. transient transfection techniques were used to knock down and modulate EPRS in these cells. EPRS is expressed at higher levels in OSCC than in normal tissues, and it predicts poor prognosis in patients. In a nude mouse xenograft model, evodiamine inhibited tumor growth and the expression of EPRS. Evodiamine impacted cell proliferation, glutamine metabolism, and EPRS expression on Cal27 and SAS cell lines. In EPRS knockdown cell lines, both cell proliferation and glutamine metabolism are suppressed. EPRS's overexpression partially restores evodiamine's inhibitory effects on cell proliferation and glutamine metabolism. This study provides crucial experimental evidence supporting the potential therapeutic application of evodiamine in treating OSCC. Evodiamine exhibits promising anti‐tumor effects by targeting EPRS to regulate glutamate metabolism.https://doi.org/10.1002/kjm2.12803evodiamineglutamate metabolismglutamyl‐prolyl‐tRNA synthetase 1 (EPRS)oral squamous cell carcinomaproliferation |
| spellingShingle | Li‐Qi Lin Si‐Yi Lv Hao‐Zhe Ren Rong‐Rong Li Lin Li Yun‐Qing Pang Jing Wang Evodiamine inhibits EPRS expression to regulate glutamate metabolism and proliferation of oral squamous cell carcinoma cells Kaohsiung Journal of Medical Sciences evodiamine glutamate metabolism glutamyl‐prolyl‐tRNA synthetase 1 (EPRS) oral squamous cell carcinoma proliferation |
| title | Evodiamine inhibits EPRS expression to regulate glutamate metabolism and proliferation of oral squamous cell carcinoma cells |
| title_full | Evodiamine inhibits EPRS expression to regulate glutamate metabolism and proliferation of oral squamous cell carcinoma cells |
| title_fullStr | Evodiamine inhibits EPRS expression to regulate glutamate metabolism and proliferation of oral squamous cell carcinoma cells |
| title_full_unstemmed | Evodiamine inhibits EPRS expression to regulate glutamate metabolism and proliferation of oral squamous cell carcinoma cells |
| title_short | Evodiamine inhibits EPRS expression to regulate glutamate metabolism and proliferation of oral squamous cell carcinoma cells |
| title_sort | evodiamine inhibits eprs expression to regulate glutamate metabolism and proliferation of oral squamous cell carcinoma cells |
| topic | evodiamine glutamate metabolism glutamyl‐prolyl‐tRNA synthetase 1 (EPRS) oral squamous cell carcinoma proliferation |
| url | https://doi.org/10.1002/kjm2.12803 |
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