Effects of Pyriproxyfen Exposure on Reproduction and Gene Expressions in Silkworm, <i>Bombyx mori</i>
The silkworm, <i>Bombyx mori</i> Linnaeus, is an important economic insect and a representative model organism of Lepidoptera, which has been widely used in the study of reproduction and development. The development of the silkworm’s reproductive gland is easily affected by many external...
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MDPI AG
2020-07-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/8/467 |
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| author | He-Ying Qian Xiao Zhang Guo-Dong Zhao Hui-Min Guo Gang Li An-Ying Xu |
| author_facet | He-Ying Qian Xiao Zhang Guo-Dong Zhao Hui-Min Guo Gang Li An-Ying Xu |
| author_sort | He-Ying Qian |
| collection | DOAJ |
| description | The silkworm, <i>Bombyx mori</i> Linnaeus, is an important economic insect and a representative model organism of Lepidoptera, which has been widely used in the study of reproduction and development. The development of the silkworm’s reproductive gland is easily affected by many external factors, such as chemical insecticides. After the silkworm larvae were treated with different concentrations of pyriproxyfen, the results showed that the number of eggs and hatching rate of eggs in the silkworm can be reduced by pyriproxyfen, and the concentration effects were displayed. Pyriproxyfen exposure could affect the normal development of the ovary tissue by reducing the number of oocytes and oogonia in the ovaries of silkworm fed with pyriproxyfen. We employed qRT-PCR, to detect the expressions of genes related to ovary development (<i>Vg</i>, <i>Ovo, Otu</i>, <i>Sxl-S</i> and <i>Sxl-L</i>) and hormone regulation (<i>EcR</i> and <i>JHBP2</i>) in silkworm. Our study showed that the transcription levels of <i>Vg</i>, <i>Ovo, Otu</i>, <i>Sxl-S</i> and <i>Sxl-L</i> in the treatment group were lower than those in the control group (6.08%, 61.99%, 83.51%, 99.31% and 71.95%, respectively). The transcription level of <i>ECR</i> was 70.22% for the control group, while that of <i>JHBP2</i> was upregulated by 3.92-fold. Changes of transcription levels of these genes caused by pyriproxyfen exposure ultimately affect the absorption of nutrients, energy metabolism, ovary development and egg formation of the silkworm, thus leading to reproductive disorders of the silkworm. In general, our study revealed the response of silkworm reproduction to pyriproxyfen exposure and provided a certain reference value for the metabolism of the silkworm to pyriproxyfen. |
| first_indexed | 2024-03-10T18:14:50Z |
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| language | English |
| last_indexed | 2024-03-10T18:14:50Z |
| publishDate | 2020-07-01 |
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| spelling | doaj.art-43f57822cec34fde917a5f077d032b8e2023-11-20T07:46:25ZengMDPI AGInsects2075-44502020-07-0111846710.3390/insects11080467Effects of Pyriproxyfen Exposure on Reproduction and Gene Expressions in Silkworm, <i>Bombyx mori</i>He-Ying Qian0Xiao Zhang1Guo-Dong Zhao2Hui-Min Guo3Gang Li4An-Ying Xu5School of Biotechnology, Jiangsu University of Science and Technology, Jiangsu 212018, ChinaSchool of Biotechnology, Jiangsu University of Science and Technology, Jiangsu 212018, ChinaSchool of Biotechnology, Jiangsu University of Science and Technology, Jiangsu 212018, ChinaSchool of Biotechnology, Jiangsu University of Science and Technology, Jiangsu 212018, ChinaSchool of Biotechnology, Jiangsu University of Science and Technology, Jiangsu 212018, ChinaSchool of Biotechnology, Jiangsu University of Science and Technology, Jiangsu 212018, ChinaThe silkworm, <i>Bombyx mori</i> Linnaeus, is an important economic insect and a representative model organism of Lepidoptera, which has been widely used in the study of reproduction and development. The development of the silkworm’s reproductive gland is easily affected by many external factors, such as chemical insecticides. After the silkworm larvae were treated with different concentrations of pyriproxyfen, the results showed that the number of eggs and hatching rate of eggs in the silkworm can be reduced by pyriproxyfen, and the concentration effects were displayed. Pyriproxyfen exposure could affect the normal development of the ovary tissue by reducing the number of oocytes and oogonia in the ovaries of silkworm fed with pyriproxyfen. We employed qRT-PCR, to detect the expressions of genes related to ovary development (<i>Vg</i>, <i>Ovo, Otu</i>, <i>Sxl-S</i> and <i>Sxl-L</i>) and hormone regulation (<i>EcR</i> and <i>JHBP2</i>) in silkworm. Our study showed that the transcription levels of <i>Vg</i>, <i>Ovo, Otu</i>, <i>Sxl-S</i> and <i>Sxl-L</i> in the treatment group were lower than those in the control group (6.08%, 61.99%, 83.51%, 99.31% and 71.95%, respectively). The transcription level of <i>ECR</i> was 70.22% for the control group, while that of <i>JHBP2</i> was upregulated by 3.92-fold. Changes of transcription levels of these genes caused by pyriproxyfen exposure ultimately affect the absorption of nutrients, energy metabolism, ovary development and egg formation of the silkworm, thus leading to reproductive disorders of the silkworm. In general, our study revealed the response of silkworm reproduction to pyriproxyfen exposure and provided a certain reference value for the metabolism of the silkworm to pyriproxyfen.https://www.mdpi.com/2075-4450/11/8/467<i>Bombyx mori</i>gene expressionhormone regulationovary developmentreproduction |
| spellingShingle | He-Ying Qian Xiao Zhang Guo-Dong Zhao Hui-Min Guo Gang Li An-Ying Xu Effects of Pyriproxyfen Exposure on Reproduction and Gene Expressions in Silkworm, <i>Bombyx mori</i> Insects <i>Bombyx mori</i> gene expression hormone regulation ovary development reproduction |
| title | Effects of Pyriproxyfen Exposure on Reproduction and Gene Expressions in Silkworm, <i>Bombyx mori</i> |
| title_full | Effects of Pyriproxyfen Exposure on Reproduction and Gene Expressions in Silkworm, <i>Bombyx mori</i> |
| title_fullStr | Effects of Pyriproxyfen Exposure on Reproduction and Gene Expressions in Silkworm, <i>Bombyx mori</i> |
| title_full_unstemmed | Effects of Pyriproxyfen Exposure on Reproduction and Gene Expressions in Silkworm, <i>Bombyx mori</i> |
| title_short | Effects of Pyriproxyfen Exposure on Reproduction and Gene Expressions in Silkworm, <i>Bombyx mori</i> |
| title_sort | effects of pyriproxyfen exposure on reproduction and gene expressions in silkworm i bombyx mori i |
| topic | <i>Bombyx mori</i> gene expression hormone regulation ovary development reproduction |
| url | https://www.mdpi.com/2075-4450/11/8/467 |
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