Peptide-fluorescent bacteria complex as luminescent reagents for cancer diagnosis.
Currently in clinic, people use hematoxylin and eosin stain (H&E stain) and immunohistochemistry methods to identify the generation and genre of cancers for human pathological samples. Since these methods are inaccurate and time consuming, developing a rapid and accurate method to detect cancer...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3548802?pdf=render |
| _version_ | 1828369820599451648 |
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| author | Bing Dong Anxin Wang Lihua Yuan Lisha Chen Kefeng Pu Wei Duan Xiyun Yan Yimin Zhu |
| author_facet | Bing Dong Anxin Wang Lihua Yuan Lisha Chen Kefeng Pu Wei Duan Xiyun Yan Yimin Zhu |
| author_sort | Bing Dong |
| collection | DOAJ |
| description | Currently in clinic, people use hematoxylin and eosin stain (H&E stain) and immunohistochemistry methods to identify the generation and genre of cancers for human pathological samples. Since these methods are inaccurate and time consuming, developing a rapid and accurate method to detect cancer is urgently demanded. In our study, binding peptides for lung cancer cell line A549 were identified using bacteria surface display method. With those binding peptides for A549 cells on the surface, the fluorescent bacteria (Escherichia coli with stably expressed green fluorescent protein) were served as specific detecting reagents for the diagnosis of cancers. The binding activity of peptide-fluorescent bacteria complex was confirmed by detached cancer cells, attached cancer cells and mice tumor xenograft samples. A unique fixation method was developed for peptide-bacteria complex in order to make this complex more feasible for the clinic use. This peptide-fluorescent bacteria complex has great potential to become a new diagnostic tool for clinical application. |
| first_indexed | 2024-04-14T06:29:42Z |
| format | Article |
| id | doaj.art-43f706b3f9cb4ad1a22a4a601f541a7a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T06:29:42Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-43f706b3f9cb4ad1a22a4a601f541a7a2022-12-22T02:07:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5446710.1371/journal.pone.0054467Peptide-fluorescent bacteria complex as luminescent reagents for cancer diagnosis.Bing DongAnxin WangLihua YuanLisha ChenKefeng PuWei DuanXiyun YanYimin ZhuCurrently in clinic, people use hematoxylin and eosin stain (H&E stain) and immunohistochemistry methods to identify the generation and genre of cancers for human pathological samples. Since these methods are inaccurate and time consuming, developing a rapid and accurate method to detect cancer is urgently demanded. In our study, binding peptides for lung cancer cell line A549 were identified using bacteria surface display method. With those binding peptides for A549 cells on the surface, the fluorescent bacteria (Escherichia coli with stably expressed green fluorescent protein) were served as specific detecting reagents for the diagnosis of cancers. The binding activity of peptide-fluorescent bacteria complex was confirmed by detached cancer cells, attached cancer cells and mice tumor xenograft samples. A unique fixation method was developed for peptide-bacteria complex in order to make this complex more feasible for the clinic use. This peptide-fluorescent bacteria complex has great potential to become a new diagnostic tool for clinical application.http://europepmc.org/articles/PMC3548802?pdf=render |
| spellingShingle | Bing Dong Anxin Wang Lihua Yuan Lisha Chen Kefeng Pu Wei Duan Xiyun Yan Yimin Zhu Peptide-fluorescent bacteria complex as luminescent reagents for cancer diagnosis. PLoS ONE |
| title | Peptide-fluorescent bacteria complex as luminescent reagents for cancer diagnosis. |
| title_full | Peptide-fluorescent bacteria complex as luminescent reagents for cancer diagnosis. |
| title_fullStr | Peptide-fluorescent bacteria complex as luminescent reagents for cancer diagnosis. |
| title_full_unstemmed | Peptide-fluorescent bacteria complex as luminescent reagents for cancer diagnosis. |
| title_short | Peptide-fluorescent bacteria complex as luminescent reagents for cancer diagnosis. |
| title_sort | peptide fluorescent bacteria complex as luminescent reagents for cancer diagnosis |
| url | http://europepmc.org/articles/PMC3548802?pdf=render |
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