Over-Expression of the Cell-Cycle Gene <i>LaCDKB1;2</i> Promotes Cell Proliferation and the Formation of Normal Cotyledonary Embryos during <i>Larix kaempferi</i> Somatic Embryogenesis
Somatic embryogenesis is an effective tool for the production of forest tree seedlings with desirable characteristics; however, the low initiation frequency and productivity of high-quality mature somatic embryos are still limiting factors for <i>Larix kaempferi</i> (Japanese larch). Her...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
|
| Series: | Genes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4425/12/9/1435 |
| _version_ | 1797519113103343616 |
|---|---|
| author | Yanhui Kang Wanfeng Li Lifeng Zhang Liwang Qi |
| author_facet | Yanhui Kang Wanfeng Li Lifeng Zhang Liwang Qi |
| author_sort | Yanhui Kang |
| collection | DOAJ |
| description | Somatic embryogenesis is an effective tool for the production of forest tree seedlings with desirable characteristics; however, the low initiation frequency and productivity of high-quality mature somatic embryos are still limiting factors for <i>Larix kaempferi</i> (Japanese larch). Here, we analyzed the expression pattern of <i>L</i><i>. kaempferi</i> <i>cyclin-dependent kinase B 1;2</i> (<i>LaCDKB1;2</i>) during somatic embryogenesis in <i>L. kaempferi</i> and its relationship with the cell proliferation rate. We also analyzed the effect of <i>LaCDKB1;2</i> over-expression on somatic embryo quality. The results revealed a positive correlation between <i>LaCDKB1;2</i> expression and the cell proliferation rate during the proliferation stage. After <i>LaCDKB1;2</i> over-expression, the proliferation rate of cultures increased, and the number of somatic embryos in transgenic cultures was 2.69 times that in non-transformed cultures. Notably, the number of normal cotyledonary embryos in transgenic cultures was 3 times that in non-transformed cultures, indicating that <i>LaCDKB1;2</i> not only increases the proliferation of cultures and the number of somatic embryos but also improves the quality of somatic embryos. These results provide insight into the regulatory mechanisms of somatic embryogenesis as well as new <i>Larix</i> breeding material. |
| first_indexed | 2024-03-10T07:38:32Z |
| format | Article |
| id | doaj.art-43f7aa3235d7464cafb4a0f317ad36e3 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T07:38:32Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-43f7aa3235d7464cafb4a0f317ad36e32023-11-22T13:15:06ZengMDPI AGGenes2073-44252021-09-01129143510.3390/genes12091435Over-Expression of the Cell-Cycle Gene <i>LaCDKB1;2</i> Promotes Cell Proliferation and the Formation of Normal Cotyledonary Embryos during <i>Larix kaempferi</i> Somatic EmbryogenesisYanhui Kang0Wanfeng Li1Lifeng Zhang2Liwang Qi3State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaSomatic embryogenesis is an effective tool for the production of forest tree seedlings with desirable characteristics; however, the low initiation frequency and productivity of high-quality mature somatic embryos are still limiting factors for <i>Larix kaempferi</i> (Japanese larch). Here, we analyzed the expression pattern of <i>L</i><i>. kaempferi</i> <i>cyclin-dependent kinase B 1;2</i> (<i>LaCDKB1;2</i>) during somatic embryogenesis in <i>L. kaempferi</i> and its relationship with the cell proliferation rate. We also analyzed the effect of <i>LaCDKB1;2</i> over-expression on somatic embryo quality. The results revealed a positive correlation between <i>LaCDKB1;2</i> expression and the cell proliferation rate during the proliferation stage. After <i>LaCDKB1;2</i> over-expression, the proliferation rate of cultures increased, and the number of somatic embryos in transgenic cultures was 2.69 times that in non-transformed cultures. Notably, the number of normal cotyledonary embryos in transgenic cultures was 3 times that in non-transformed cultures, indicating that <i>LaCDKB1;2</i> not only increases the proliferation of cultures and the number of somatic embryos but also improves the quality of somatic embryos. These results provide insight into the regulatory mechanisms of somatic embryogenesis as well as new <i>Larix</i> breeding material.https://www.mdpi.com/2073-4425/12/9/1435<i>Larix kaempferi</i><i>LaCDKB12</i>cell-cycle genesomatic embryogenesis |
| spellingShingle | Yanhui Kang Wanfeng Li Lifeng Zhang Liwang Qi Over-Expression of the Cell-Cycle Gene <i>LaCDKB1;2</i> Promotes Cell Proliferation and the Formation of Normal Cotyledonary Embryos during <i>Larix kaempferi</i> Somatic Embryogenesis Genes <i>Larix kaempferi</i> <i>LaCDKB1 2</i> cell-cycle gene somatic embryogenesis |
| title | Over-Expression of the Cell-Cycle Gene <i>LaCDKB1;2</i> Promotes Cell Proliferation and the Formation of Normal Cotyledonary Embryos during <i>Larix kaempferi</i> Somatic Embryogenesis |
| title_full | Over-Expression of the Cell-Cycle Gene <i>LaCDKB1;2</i> Promotes Cell Proliferation and the Formation of Normal Cotyledonary Embryos during <i>Larix kaempferi</i> Somatic Embryogenesis |
| title_fullStr | Over-Expression of the Cell-Cycle Gene <i>LaCDKB1;2</i> Promotes Cell Proliferation and the Formation of Normal Cotyledonary Embryos during <i>Larix kaempferi</i> Somatic Embryogenesis |
| title_full_unstemmed | Over-Expression of the Cell-Cycle Gene <i>LaCDKB1;2</i> Promotes Cell Proliferation and the Formation of Normal Cotyledonary Embryos during <i>Larix kaempferi</i> Somatic Embryogenesis |
| title_short | Over-Expression of the Cell-Cycle Gene <i>LaCDKB1;2</i> Promotes Cell Proliferation and the Formation of Normal Cotyledonary Embryos during <i>Larix kaempferi</i> Somatic Embryogenesis |
| title_sort | over expression of the cell cycle gene i lacdkb1 2 i promotes cell proliferation and the formation of normal cotyledonary embryos during i larix kaempferi i somatic embryogenesis |
| topic | <i>Larix kaempferi</i> <i>LaCDKB1 2</i> cell-cycle gene somatic embryogenesis |
| url | https://www.mdpi.com/2073-4425/12/9/1435 |
| work_keys_str_mv | AT yanhuikang overexpressionofthecellcyclegeneilacdkb12ipromotescellproliferationandtheformationofnormalcotyledonaryembryosduringilarixkaempferiisomaticembryogenesis AT wanfengli overexpressionofthecellcyclegeneilacdkb12ipromotescellproliferationandtheformationofnormalcotyledonaryembryosduringilarixkaempferiisomaticembryogenesis AT lifengzhang overexpressionofthecellcyclegeneilacdkb12ipromotescellproliferationandtheformationofnormalcotyledonaryembryosduringilarixkaempferiisomaticembryogenesis AT liwangqi overexpressionofthecellcyclegeneilacdkb12ipromotescellproliferationandtheformationofnormalcotyledonaryembryosduringilarixkaempferiisomaticembryogenesis |