Analysis of the PPARD gene expression level changes in football players in response to the training cycle
The PPARD gene codes protein that belongs to the peroxisome proliferator-activated receptor (PPAR) family engaged in a variety of biological processes, including lipid metabolism in muscle cells. In this study, we assess the relationship between PPARD gene expression lipid metabolism parameters and...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2018-10-01
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| Series: | Balkan Journal of Medical Genetics |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/bjmg-2018-0008 |
| _version_ | 1797718664988852224 |
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| author | Domańska-Senderowska D Snochowska A Szmigielska P Jastrzębski Z Jegier A Kiszałkiewicz J Dróbka K Jastrzębska J Pastuszak-Lewandoska D Cięszczyk P Maciejewska-Skrendo A Zmijewski P Brzeziańska-Lasota E |
| author_facet | Domańska-Senderowska D Snochowska A Szmigielska P Jastrzębski Z Jegier A Kiszałkiewicz J Dróbka K Jastrzębska J Pastuszak-Lewandoska D Cięszczyk P Maciejewska-Skrendo A Zmijewski P Brzeziańska-Lasota E |
| author_sort | Domańska-Senderowska D |
| collection | DOAJ |
| description | The PPARD gene codes protein that belongs to the peroxisome proliferator-activated receptor (PPAR) family engaged in a variety of biological processes, including lipid metabolism in muscle cells. In this study, we assess the relationship between PPARD gene expression lipid metabolism parameters and the variation of the PPARD gene expression before (T1) and after 12 hours of training (T2) sessions in a group of football players. Peripheral blood lymphocytes were obtained from 22 football players (17.5±0.7 years, 178±0.7 cm, 68.05±9.18 kg). The PPARD gene expression, analyzed by quantitative polymerase chain reaction (qPCR), was significantly higher after T2 (p = 0.0006). Moreover, at the end of the training cycle, there was a significant decrease in relative fat tissue (FAT) (%) (p = 0.01) and absolute FAT (kg) (p = 0.01). A negative correlation was observed between absolute FAT (kg) and PPARD gene expression level in T2 (p = 0.03). The levels of cholesterol and triglyceride (TG) fractions were not significantly different (p >0.05) before and after training. No significant relationship between PPARD expression and cholesterol or TG levels was found. We found that physical training affects PPARD expression. Moreover, the negative correlation between PPARD expression and absolute FAT (kg) level may be indicative of the contribution of PPARD in metabolic adaptation to increased lipid uptake that can be used to control the body composition of athletes. |
| first_indexed | 2024-03-12T08:53:41Z |
| format | Article |
| id | doaj.art-43f7b0de43f6455ebc006e188ea04b20 |
| institution | Directory Open Access Journal |
| issn | 1311-0160 |
| language | English |
| last_indexed | 2024-03-12T08:53:41Z |
| publishDate | 2018-10-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Balkan Journal of Medical Genetics |
| spelling | doaj.art-43f7b0de43f6455ebc006e188ea04b202023-09-02T16:10:18ZengSciendoBalkan Journal of Medical Genetics1311-01602018-10-01211192510.2478/bjmg-2018-0008bjmg-2018-0008Analysis of the PPARD gene expression level changes in football players in response to the training cycleDomańska-Senderowska D0Snochowska A1Szmigielska P2Jastrzębski Z3Jegier A4Kiszałkiewicz J5Dróbka K6Jastrzębska J7Pastuszak-Lewandoska D8Cięszczyk P9Maciejewska-Skrendo A10Zmijewski P11Brzeziańska-Lasota E12Department of Biomedicine and Genetics, Chair of Biology and Medical Microbiology, Medical University of Lodz, Lodz, PolandDepartment of Biomedicine and Genetics, Chair of Biology and Medical Microbiology, Medical University of Lodz, Lodz, PolandDepartment of Biomedicine and Genetics, Chair of Biology and Medical Microbiology, Medical University of Lodz, Lodz, PolandFaculty of Tourism and Recreation, Gdansk University of Physical Education and Sport, Gdansk, PolandDepartment of Sport Medicine, Medical University of Lodz, Lodz, PolandDepartment of Biomedicine and Genetics, Chair of Biology and Medical Microbiology, Medical University of Lodz, Lodz, PolandDepartment of Biomedicine and Genetics, Chair of Biology and Medical Microbiology, Medical University of Lodz, Lodz, PolandFaculty of Tourism and Recreation, Gdansk University of Physical Education and Sport, Gdansk, PolandDepartment of Biomedicine and Genetics, Chair of Biology and Medical Microbiology, Medical University of Lodz, Lodz, PolandDepartment of Nutrition, the Jerzy Kukuczka Academy of Physical Education in Kraków, Katowice, PolandFaculty of Physical Education, Gdansk University of Physical, Education and Sport, Gdansk, PolandFaculty of Medicine, University of Information Technology and Management in Rzeszow, Rzeszow, PolandDepartment of Biomedicine and Genetics, Chair of Biology and Medical Microbiology, Medical University of Lodz, Lodz, PolandThe PPARD gene codes protein that belongs to the peroxisome proliferator-activated receptor (PPAR) family engaged in a variety of biological processes, including lipid metabolism in muscle cells. In this study, we assess the relationship between PPARD gene expression lipid metabolism parameters and the variation of the PPARD gene expression before (T1) and after 12 hours of training (T2) sessions in a group of football players. Peripheral blood lymphocytes were obtained from 22 football players (17.5±0.7 years, 178±0.7 cm, 68.05±9.18 kg). The PPARD gene expression, analyzed by quantitative polymerase chain reaction (qPCR), was significantly higher after T2 (p = 0.0006). Moreover, at the end of the training cycle, there was a significant decrease in relative fat tissue (FAT) (%) (p = 0.01) and absolute FAT (kg) (p = 0.01). A negative correlation was observed between absolute FAT (kg) and PPARD gene expression level in T2 (p = 0.03). The levels of cholesterol and triglyceride (TG) fractions were not significantly different (p >0.05) before and after training. No significant relationship between PPARD expression and cholesterol or TG levels was found. We found that physical training affects PPARD expression. Moreover, the negative correlation between PPARD expression and absolute FAT (kg) level may be indicative of the contribution of PPARD in metabolic adaptation to increased lipid uptake that can be used to control the body composition of athletes.https://doi.org/10.2478/bjmg-2018-0008body fatexercisegene expressionlipid metabolismperoxisome proliferator-activated receptor (ppar)sports |
| spellingShingle | Domańska-Senderowska D Snochowska A Szmigielska P Jastrzębski Z Jegier A Kiszałkiewicz J Dróbka K Jastrzębska J Pastuszak-Lewandoska D Cięszczyk P Maciejewska-Skrendo A Zmijewski P Brzeziańska-Lasota E Analysis of the PPARD gene expression level changes in football players in response to the training cycle Balkan Journal of Medical Genetics body fat exercise gene expression lipid metabolism peroxisome proliferator-activated receptor (ppar) sports |
| title | Analysis of the PPARD gene expression level changes in football players in response to the training cycle |
| title_full | Analysis of the PPARD gene expression level changes in football players in response to the training cycle |
| title_fullStr | Analysis of the PPARD gene expression level changes in football players in response to the training cycle |
| title_full_unstemmed | Analysis of the PPARD gene expression level changes in football players in response to the training cycle |
| title_short | Analysis of the PPARD gene expression level changes in football players in response to the training cycle |
| title_sort | analysis of the ppard gene expression level changes in football players in response to the training cycle |
| topic | body fat exercise gene expression lipid metabolism peroxisome proliferator-activated receptor (ppar) sports |
| url | https://doi.org/10.2478/bjmg-2018-0008 |
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