Experimental Introgression To Evaluate the Impact of Sex Specific Traits on Drosophila melanogaster Incipient Speciation
Sex specific traits are involved in speciation but it is difficult to determine whether their variation initiates or reinforces sexual isolation. In some insects, speciation depends of the rapid change of expression in desaturase genes coding for sex pheromones. Two closely related desaturase genes...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2019-08-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.119.400385 |
| _version_ | 1818913278993104896 |
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| author | Jérôme Cortot Jean-Pierre Farine Benjamin Houot Claude Everaerts Jean-François Ferveur |
| author_facet | Jérôme Cortot Jean-Pierre Farine Benjamin Houot Claude Everaerts Jean-François Ferveur |
| author_sort | Jérôme Cortot |
| collection | DOAJ |
| description | Sex specific traits are involved in speciation but it is difficult to determine whether their variation initiates or reinforces sexual isolation. In some insects, speciation depends of the rapid change of expression in desaturase genes coding for sex pheromones. Two closely related desaturase genes are involved in Drosophila melanogaster pheromonal communication: desat1 affects both the production and the reception of sex pheromones while desat2 is involved in their production in flies of Zimbabwe populations. There is a strong asymmetric sexual isolation between Zimbabwe populations and all other “Cosmopolitan” populations: Zimbabwe females rarely copulate with Cosmopolitan males whereas Zimbabwe males readily copulate with all females. All populations express desat1 but only Zimbabwe strains show high desat2 expression. To evaluate the impact of sex pheromones, female receptivity and desat expression on the incipient speciation process between Zimbabwe and Cosmopolitan populations, we introgressed the Zimbabwe genome into a Cosmopolitan genome labeled with the white mutation, using a multi-generation procedure. The association between these sex-specific traits was determined during the procedure. The production of pheromones was largely dissociated between the sexes. The copulation frequency (but not latency) was highly correlated with the female—but not with the male—principal pheromones. We finally obtained two stable white lines showing Zimbabwe-like sex pheromones, copulation discrimination and desat expression. Our study indicates that the variation of sex pheromones and mating discrimination depend of distinct—yet overlapping—sets of genes in each sex suggesting that their cumulated effects participate to reinforce the speciation process. |
| first_indexed | 2024-12-19T23:27:57Z |
| format | Article |
| id | doaj.art-43f9a897910c4fb684b94202f6280a8a |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-19T23:27:57Z |
| publishDate | 2019-08-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-43f9a897910c4fb684b94202f6280a8a2022-12-21T20:01:48ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362019-08-01982561257210.1534/g3.119.40038517Experimental Introgression To Evaluate the Impact of Sex Specific Traits on Drosophila melanogaster Incipient SpeciationJérôme CortotJean-Pierre FarineBenjamin HouotClaude EveraertsJean-François FerveurSex specific traits are involved in speciation but it is difficult to determine whether their variation initiates or reinforces sexual isolation. In some insects, speciation depends of the rapid change of expression in desaturase genes coding for sex pheromones. Two closely related desaturase genes are involved in Drosophila melanogaster pheromonal communication: desat1 affects both the production and the reception of sex pheromones while desat2 is involved in their production in flies of Zimbabwe populations. There is a strong asymmetric sexual isolation between Zimbabwe populations and all other “Cosmopolitan” populations: Zimbabwe females rarely copulate with Cosmopolitan males whereas Zimbabwe males readily copulate with all females. All populations express desat1 but only Zimbabwe strains show high desat2 expression. To evaluate the impact of sex pheromones, female receptivity and desat expression on the incipient speciation process between Zimbabwe and Cosmopolitan populations, we introgressed the Zimbabwe genome into a Cosmopolitan genome labeled with the white mutation, using a multi-generation procedure. The association between these sex-specific traits was determined during the procedure. The production of pheromones was largely dissociated between the sexes. The copulation frequency (but not latency) was highly correlated with the female—but not with the male—principal pheromones. We finally obtained two stable white lines showing Zimbabwe-like sex pheromones, copulation discrimination and desat expression. Our study indicates that the variation of sex pheromones and mating discrimination depend of distinct—yet overlapping—sets of genes in each sex suggesting that their cumulated effects participate to reinforce the speciation process.http://g3journal.org/lookup/doi/10.1534/g3.119.400385cuticular hydrocarbontricoseneheptacosadiene |
| spellingShingle | Jérôme Cortot Jean-Pierre Farine Benjamin Houot Claude Everaerts Jean-François Ferveur Experimental Introgression To Evaluate the Impact of Sex Specific Traits on Drosophila melanogaster Incipient Speciation G3: Genes, Genomes, Genetics cuticular hydrocarbon tricosene heptacosadiene |
| title | Experimental Introgression To Evaluate the Impact of Sex Specific Traits on Drosophila melanogaster Incipient Speciation |
| title_full | Experimental Introgression To Evaluate the Impact of Sex Specific Traits on Drosophila melanogaster Incipient Speciation |
| title_fullStr | Experimental Introgression To Evaluate the Impact of Sex Specific Traits on Drosophila melanogaster Incipient Speciation |
| title_full_unstemmed | Experimental Introgression To Evaluate the Impact of Sex Specific Traits on Drosophila melanogaster Incipient Speciation |
| title_short | Experimental Introgression To Evaluate the Impact of Sex Specific Traits on Drosophila melanogaster Incipient Speciation |
| title_sort | experimental introgression to evaluate the impact of sex specific traits on drosophila melanogaster incipient speciation |
| topic | cuticular hydrocarbon tricosene heptacosadiene |
| url | http://g3journal.org/lookup/doi/10.1534/g3.119.400385 |
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