Tissue-specific DNA methylation in Haemanthus katharinae Bak. (Amaryllidaceae)
The level of DNA methylation was compared in root meristem, adult leaf and endosperm of monocotyledonous species, Haemanthus katharinae, with the use of HPLC, restriction analysis, Southern blot hybridization and in situ nick-translation driven by restriction enzyme HhaI The highest level of 5-methy...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2014-01-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/828 |
| _version_ | 1831702444639256576 |
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| author | Tomasz Sakowicz Maria J. Olszewska Piotr Łuchniak Joanna Kaźmierczak |
| author_facet | Tomasz Sakowicz Maria J. Olszewska Piotr Łuchniak Joanna Kaźmierczak |
| author_sort | Tomasz Sakowicz |
| collection | DOAJ |
| description | The level of DNA methylation was compared in root meristem, adult leaf and endosperm of monocotyledonous species, Haemanthus katharinae, with the use of HPLC, restriction analysis, Southern blot hybridization and in situ nick-translation driven by restriction enzyme HhaI The highest level of 5-methylcytosine was observed in adult leaf whose nuclear chromatin is particularly condensed, and the lowest in endosperm. The level of DNA methylation of repetitive HaeIII 4006p sequence follows that of the total DNA. |
| first_indexed | 2024-12-20T15:21:51Z |
| format | Article |
| id | doaj.art-43fa1ecf5d874a799034c1ab04eec1e9 |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-20T15:21:51Z |
| publishDate | 2014-01-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-43fa1ecf5d874a799034c1ab04eec1e92022-12-21T19:36:01ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802014-01-0167217518010.5586/asbp.1998.020604Tissue-specific DNA methylation in Haemanthus katharinae Bak. (Amaryllidaceae)Tomasz Sakowicz0Maria J. Olszewska1Piotr Łuchniak2Joanna Kaźmierczak3University of ŁódźUniversity of ŁódźUniversity of ŁódźUniversity of ŁódźThe level of DNA methylation was compared in root meristem, adult leaf and endosperm of monocotyledonous species, Haemanthus katharinae, with the use of HPLC, restriction analysis, Southern blot hybridization and in situ nick-translation driven by restriction enzyme HhaI The highest level of 5-methylcytosine was observed in adult leaf whose nuclear chromatin is particularly condensed, and the lowest in endosperm. The level of DNA methylation of repetitive HaeIII 4006p sequence follows that of the total DNA.https://pbsociety.org.pl/journals/index.php/asbp/article/view/828DNA methylationroot meristemleafendospermHaemanthus katharinae |
| spellingShingle | Tomasz Sakowicz Maria J. Olszewska Piotr Łuchniak Joanna Kaźmierczak Tissue-specific DNA methylation in Haemanthus katharinae Bak. (Amaryllidaceae) Acta Societatis Botanicorum Poloniae DNA methylation root meristem leaf endosperm Haemanthus katharinae |
| title | Tissue-specific DNA methylation in Haemanthus katharinae Bak. (Amaryllidaceae) |
| title_full | Tissue-specific DNA methylation in Haemanthus katharinae Bak. (Amaryllidaceae) |
| title_fullStr | Tissue-specific DNA methylation in Haemanthus katharinae Bak. (Amaryllidaceae) |
| title_full_unstemmed | Tissue-specific DNA methylation in Haemanthus katharinae Bak. (Amaryllidaceae) |
| title_short | Tissue-specific DNA methylation in Haemanthus katharinae Bak. (Amaryllidaceae) |
| title_sort | tissue specific dna methylation in haemanthus katharinae bak amaryllidaceae |
| topic | DNA methylation root meristem leaf endosperm Haemanthus katharinae |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/828 |
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