Leishman-Giemsa Cocktail - Is it an Effective Stain for Air Dried Cytology Smears
Introduction: Air dried cytology smears are stained routinely with Romanowsky stains so that the relative cell size, nuclear size, cytoplasmic details, smear background elements and intercellular matrix components are better appreciated. A variety of modified Romanowsky stains are used in cytolo...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2017-03-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/9490/25553_CE[Ra1]_F(DK)_PF1(NE_SY_DK)_PFA(DK)_PF2(RB_SY_DK).pdf |
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| author | Shilpa Manigatta Doddagowda Hemalatha Anantharamaiah Shashidhar Chinaiah Subramanyam Babu Rajendra Prasad |
| author_facet | Shilpa Manigatta Doddagowda Hemalatha Anantharamaiah Shashidhar Chinaiah Subramanyam Babu Rajendra Prasad |
| author_sort | Shilpa Manigatta Doddagowda |
| collection | DOAJ |
| description | Introduction: Air dried cytology smears are stained routinely
with Romanowsky stains so that the relative cell size, nuclear
size, cytoplasmic details, smear background elements and
intercellular matrix components are better appreciated. A
variety of modified Romanowsky stains are used in cytology.
Leishman-Giemsa (LG) cocktail is one of the new staining
techniques which can be used for staining the air dried cytology
smears.
Aim: To evaluate the quality of staining of LG cocktail on air
dried smears and to compare the quality of staining of LG
cocktail with May Grunwald Giemsa (MGG) which is the most
commonly used stain in cytology.
Materials and Methods: The present prospective comparative
study was carried out with 100 cases and two extra smears were
prepared for each case and stained with MGG and LG cocktail
stains. The stained slides were blinded and were evaluated
for the staining characteristics of the nucleus, cytoplasm and
background staining. Based on this, scoring was done by two
pathologists independently. Quality Index (QI) was calculated
by dividing the scores obtained with the total score possible.
Results: LG cocktail stained slides were excellent in cytoplasmic
staining, granularity, nuclear morphology, background material
staining and overall staining characteristics. QI of LG cocktail
was 0.8 while that of MGG was 0.59.
Conclusion: Staining of air dried smears by LG cocktail has a
good QI. It is also cheaper, requires short duration for staining
as compared to MGG. Hence, LG cocktail can be an effective
replacement for MGG for staining the air dried cytology
smears. |
| first_indexed | 2024-12-19T20:45:16Z |
| format | Article |
| id | doaj.art-43fc0fe0c2604a8aa9212e88e1ffd3fd |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-19T20:45:16Z |
| publishDate | 2017-03-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-43fc0fe0c2604a8aa9212e88e1ffd3fd2022-12-21T20:06:17ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-03-01113EC16EC1810.7860/JCDR/2017/25553.9490Leishman-Giemsa Cocktail - Is it an Effective Stain for Air Dried Cytology SmearsShilpa Manigatta Doddagowda0Hemalatha Anantharamaiah Shashidhar1Chinaiah Subramanyam Babu Rajendra Prasad2Assistant Professor, Department of Pathology, Sri Devaraj Urs Medical College, Kolar, Karnataka, India.Associate Professor, Department of Pathology, Sri Devaraj Urs Medical College, Kolar, Karnataka, India.Professor, Department of Pathology, Sri Devaraj Urs Medical College, Kolar, Karnataka, India.Introduction: Air dried cytology smears are stained routinely with Romanowsky stains so that the relative cell size, nuclear size, cytoplasmic details, smear background elements and intercellular matrix components are better appreciated. A variety of modified Romanowsky stains are used in cytology. Leishman-Giemsa (LG) cocktail is one of the new staining techniques which can be used for staining the air dried cytology smears. Aim: To evaluate the quality of staining of LG cocktail on air dried smears and to compare the quality of staining of LG cocktail with May Grunwald Giemsa (MGG) which is the most commonly used stain in cytology. Materials and Methods: The present prospective comparative study was carried out with 100 cases and two extra smears were prepared for each case and stained with MGG and LG cocktail stains. The stained slides were blinded and were evaluated for the staining characteristics of the nucleus, cytoplasm and background staining. Based on this, scoring was done by two pathologists independently. Quality Index (QI) was calculated by dividing the scores obtained with the total score possible. Results: LG cocktail stained slides were excellent in cytoplasmic staining, granularity, nuclear morphology, background material staining and overall staining characteristics. QI of LG cocktail was 0.8 while that of MGG was 0.59. Conclusion: Staining of air dried smears by LG cocktail has a good QI. It is also cheaper, requires short duration for staining as compared to MGG. Hence, LG cocktail can be an effective replacement for MGG for staining the air dried cytology smears.https://jcdr.net/articles/PDF/9490/25553_CE[Ra1]_F(DK)_PF1(NE_SY_DK)_PFA(DK)_PF2(RB_SY_DK).pdfmay grunwald giemsaquality indexromanowsky |
| spellingShingle | Shilpa Manigatta Doddagowda Hemalatha Anantharamaiah Shashidhar Chinaiah Subramanyam Babu Rajendra Prasad Leishman-Giemsa Cocktail - Is it an Effective Stain for Air Dried Cytology Smears Journal of Clinical and Diagnostic Research may grunwald giemsa quality index romanowsky |
| title | Leishman-Giemsa Cocktail - Is it an Effective Stain for Air Dried Cytology Smears |
| title_full | Leishman-Giemsa Cocktail - Is it an Effective Stain for Air Dried Cytology Smears |
| title_fullStr | Leishman-Giemsa Cocktail - Is it an Effective Stain for Air Dried Cytology Smears |
| title_full_unstemmed | Leishman-Giemsa Cocktail - Is it an Effective Stain for Air Dried Cytology Smears |
| title_short | Leishman-Giemsa Cocktail - Is it an Effective Stain for Air Dried Cytology Smears |
| title_sort | leishman giemsa cocktail is it an effective stain for air dried cytology smears |
| topic | may grunwald giemsa quality index romanowsky |
| url | https://jcdr.net/articles/PDF/9490/25553_CE[Ra1]_F(DK)_PF1(NE_SY_DK)_PFA(DK)_PF2(RB_SY_DK).pdf |
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