A modified CUT&RUN-seq technique for qPCR analysis of chromatin-protein interactions

A modified CUT&RUN-seq technique for qPCR analysis of chromatin-protein interactions

Summary: Chromatin immunoprecipitation coupled with quantitative PCR (ChIP-qPCR) even with optimization may give low signal-to-background ratio and spatial resolution. Here, we adapted Cleavage Under Targets and Release Using Nuclease (CUT&RUN) (originally developed by the Henikoff group) to dev...

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Bibliographic Details
Main Authors: Arvind Panday, Rajula Elango, Nicholas A. Willis, Ralph Scully
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell-based Assays
Molecular Biology
Chromatin immunoprecipitation (ChIP)
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004099

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