The study of parameters of mesenchymal stromal cells differentiation in donors and patients with aplastic anemia
Aim. To examine ability of mesenchymal stromal cells (MSC) of the bone marrow (BM) for differentiation in adipogenic and osteogenic differentiation in donors and patients with aplastic anemia (AA). Material and methods. We obtained MSC cultures from BM cells of donors and AA patients and induced dif...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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"Consilium Medicum" Publishing house
2009-07-01
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| Series: | Терапевтический архив |
| Subjects: | |
| Online Access: | https://ter-arkhiv.ru/0040-3660/article/view/30413 |
| _version_ | 1819229250583003136 |
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| author | Dar'ya Anatol'evna Svinareva Tat'yana Vladimirovna Petrova Irina Nikolaevna Shipunova (Nifontova) Kira Sergeevna Momotyuk Elena Alekseevna Mikhaylova Nina Iosifovna Drize D A Svinareva T V Petrova I N Shipunova (Nifontova) K S Momotyuk E A Mikhailova N I Drize |
| author_facet | Dar'ya Anatol'evna Svinareva Tat'yana Vladimirovna Petrova Irina Nikolaevna Shipunova (Nifontova) Kira Sergeevna Momotyuk Elena Alekseevna Mikhaylova Nina Iosifovna Drize D A Svinareva T V Petrova I N Shipunova (Nifontova) K S Momotyuk E A Mikhailova N I Drize |
| author_sort | Dar'ya Anatol'evna Svinareva |
| collection | DOAJ |
| description | Aim. To examine ability of mesenchymal stromal cells (MSC) of the bone marrow (BM) for differentiation in adipogenic and osteogenic differentiation in donors and patients with aplastic anemia (AA).
Material and methods. We obtained MSC cultures from BM cells of donors and AA patients and induced differentiation of mesenchymal cells with use of relevant reagents. Morphological changes in MSC were studied with light microscopy. A relative level of expression of differentiation marker genes in MSC cultures before and after induction of differentiation was analysed with reverse transcription-polymerase chain reaction.
Results. By morphological characteristics, MSC cultures in AA patients before and after differentiation induction do not differ from donor cultures, but relative expression of the genes of differentiation markers demonstrated that expression was different in male and female donors; MSC before and after induction of differentiation differ in donors and AA patients.
Conclusion. Further studies are needed for detection of functional changes in precursors of stromal microenvironment and understanding of the disease pathogenesis. |
| first_indexed | 2024-12-23T11:10:11Z |
| format | Article |
| id | doaj.art-43fefb6ff5444a848112c6f07fec762e |
| institution | Directory Open Access Journal |
| issn | 0040-3660 2309-5342 |
| language | Russian |
| last_indexed | 2024-12-23T11:10:11Z |
| publishDate | 2009-07-01 |
| publisher | "Consilium Medicum" Publishing house |
| record_format | Article |
| series | Терапевтический архив |
| spelling | doaj.art-43fefb6ff5444a848112c6f07fec762e2022-12-21T17:49:22Zrus"Consilium Medicum" Publishing houseТерапевтический архив0040-36602309-53422009-07-01817667027441The study of parameters of mesenchymal stromal cells differentiation in donors and patients with aplastic anemiaDar'ya Anatol'evna SvinarevaTat'yana Vladimirovna PetrovaIrina Nikolaevna Shipunova (Nifontova)Kira Sergeevna MomotyukElena Alekseevna MikhaylovaNina Iosifovna DrizeD A SvinarevaT V PetrovaI N Shipunova (Nifontova)K S MomotyukE A MikhailovaN I DrizeAim. To examine ability of mesenchymal stromal cells (MSC) of the bone marrow (BM) for differentiation in adipogenic and osteogenic differentiation in donors and patients with aplastic anemia (AA). Material and methods. We obtained MSC cultures from BM cells of donors and AA patients and induced differentiation of mesenchymal cells with use of relevant reagents. Morphological changes in MSC were studied with light microscopy. A relative level of expression of differentiation marker genes in MSC cultures before and after induction of differentiation was analysed with reverse transcription-polymerase chain reaction. Results. By morphological characteristics, MSC cultures in AA patients before and after differentiation induction do not differ from donor cultures, but relative expression of the genes of differentiation markers demonstrated that expression was different in male and female donors; MSC before and after induction of differentiation differ in donors and AA patients. Conclusion. Further studies are needed for detection of functional changes in precursors of stromal microenvironment and understanding of the disease pathogenesis.https://ter-arkhiv.ru/0040-3660/article/view/30413mesenchymal stromal cellsdifferentiationgene expressionaplastic anemia |
| spellingShingle | Dar'ya Anatol'evna Svinareva Tat'yana Vladimirovna Petrova Irina Nikolaevna Shipunova (Nifontova) Kira Sergeevna Momotyuk Elena Alekseevna Mikhaylova Nina Iosifovna Drize D A Svinareva T V Petrova I N Shipunova (Nifontova) K S Momotyuk E A Mikhailova N I Drize The study of parameters of mesenchymal stromal cells differentiation in donors and patients with aplastic anemia Терапевтический архив mesenchymal stromal cells differentiation gene expression aplastic anemia |
| title | The study of parameters of mesenchymal stromal cells differentiation in donors and patients with aplastic anemia |
| title_full | The study of parameters of mesenchymal stromal cells differentiation in donors and patients with aplastic anemia |
| title_fullStr | The study of parameters of mesenchymal stromal cells differentiation in donors and patients with aplastic anemia |
| title_full_unstemmed | The study of parameters of mesenchymal stromal cells differentiation in donors and patients with aplastic anemia |
| title_short | The study of parameters of mesenchymal stromal cells differentiation in donors and patients with aplastic anemia |
| title_sort | study of parameters of mesenchymal stromal cells differentiation in donors and patients with aplastic anemia |
| topic | mesenchymal stromal cells differentiation gene expression aplastic anemia |
| url | https://ter-arkhiv.ru/0040-3660/article/view/30413 |
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