The loop2 insertion of type IX myosin acts as an electrostatic actin tether that permits processive movement.
Although class IX myosins are single-headed, they demonstrate characteristics of processive movement along actin filaments. Double-headed myosins that move processively along actin filaments achieve this by successive binding of the two heads in a hand-over-hand mechanism. This mechanism, obviously,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3887004?pdf=render |
| _version_ | 1818994697578741760 |
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| author | Kerstin Elfrink Wanqin Liao Uwe Pieper Stefanie J Oeding Martin Bähler |
| author_facet | Kerstin Elfrink Wanqin Liao Uwe Pieper Stefanie J Oeding Martin Bähler |
| author_sort | Kerstin Elfrink |
| collection | DOAJ |
| description | Although class IX myosins are single-headed, they demonstrate characteristics of processive movement along actin filaments. Double-headed myosins that move processively along actin filaments achieve this by successive binding of the two heads in a hand-over-hand mechanism. This mechanism, obviously, cannot operate in single-headed myosins. However, it has been proposed that a long class IX specific insertion in the myosin head domain at loop2 acts as an F-actin tether, allowing for single-headed processive movement. Here, we tested this proposal directly by analysing the movement of deletion constructs of the class IX myosin from Caenorhabditis elegans (Myo IX). Deletion of the large basic loop2 insertion led to a loss of processive behaviour, while deletion of the N-terminal head extension, a second unique domain of class IX myosins, did not influence the motility of Myo IX. The processive behaviour of Myo IX is also abolished with increasing salt concentrations. These observations directly demonstrate that the insertion located in loop2 acts as an electrostatic actin tether during movement of Myo IX along the actin track. |
| first_indexed | 2024-12-20T21:02:04Z |
| format | Article |
| id | doaj.art-43fffb4b84f046a4b46b5ae49ffb18f2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T21:02:04Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-43fffb4b84f046a4b46b5ae49ffb18f22022-12-21T19:26:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8487410.1371/journal.pone.0084874The loop2 insertion of type IX myosin acts as an electrostatic actin tether that permits processive movement.Kerstin ElfrinkWanqin LiaoUwe PieperStefanie J OedingMartin BählerAlthough class IX myosins are single-headed, they demonstrate characteristics of processive movement along actin filaments. Double-headed myosins that move processively along actin filaments achieve this by successive binding of the two heads in a hand-over-hand mechanism. This mechanism, obviously, cannot operate in single-headed myosins. However, it has been proposed that a long class IX specific insertion in the myosin head domain at loop2 acts as an F-actin tether, allowing for single-headed processive movement. Here, we tested this proposal directly by analysing the movement of deletion constructs of the class IX myosin from Caenorhabditis elegans (Myo IX). Deletion of the large basic loop2 insertion led to a loss of processive behaviour, while deletion of the N-terminal head extension, a second unique domain of class IX myosins, did not influence the motility of Myo IX. The processive behaviour of Myo IX is also abolished with increasing salt concentrations. These observations directly demonstrate that the insertion located in loop2 acts as an electrostatic actin tether during movement of Myo IX along the actin track.http://europepmc.org/articles/PMC3887004?pdf=render |
| spellingShingle | Kerstin Elfrink Wanqin Liao Uwe Pieper Stefanie J Oeding Martin Bähler The loop2 insertion of type IX myosin acts as an electrostatic actin tether that permits processive movement. PLoS ONE |
| title | The loop2 insertion of type IX myosin acts as an electrostatic actin tether that permits processive movement. |
| title_full | The loop2 insertion of type IX myosin acts as an electrostatic actin tether that permits processive movement. |
| title_fullStr | The loop2 insertion of type IX myosin acts as an electrostatic actin tether that permits processive movement. |
| title_full_unstemmed | The loop2 insertion of type IX myosin acts as an electrostatic actin tether that permits processive movement. |
| title_short | The loop2 insertion of type IX myosin acts as an electrostatic actin tether that permits processive movement. |
| title_sort | loop2 insertion of type ix myosin acts as an electrostatic actin tether that permits processive movement |
| url | http://europepmc.org/articles/PMC3887004?pdf=render |
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