Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent
Ergosterol (ERG) is a sterol found at high levels in cell membranes of fungi and is a smart option to be used as proof of fungal contamination in food samples. Therefore, the objective of this work was to develop a fast, sensitive and ecological methodology based on the use of hydrophobic fluorescen...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Green Analytical Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772577422000258 |
| _version_ | 1797347065341149184 |
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| author | Ricardo Elia Dazat Sabrina B. Mammana Brenda V. Canizo María Fernanda Silva Federico J.V. Gomez |
| author_facet | Ricardo Elia Dazat Sabrina B. Mammana Brenda V. Canizo María Fernanda Silva Federico J.V. Gomez |
| author_sort | Ricardo Elia Dazat |
| collection | DOAJ |
| description | Ergosterol (ERG) is a sterol found at high levels in cell membranes of fungi and is a smart option to be used as proof of fungal contamination in food samples. Therefore, the objective of this work was to develop a fast, sensitive and ecological methodology based on the use of hydrophobic fluorescent natural deep eutectic solvents (HPB-FLUODES) to determine ERG by fluorescence detection in edible mushrooms. The proposed approach involves a green sample preparation and a low cost instrumental technique leading to an efficient and simple analytical methodology suitable for high-throughput analysis (2.4 samples per minute). The experimental variables, such as the type of HPB-FLUODES, sample/solvent mass/volume ratio, time, and temperature of extraction were studied and optimized. The selected variables were HPB-FLUODES thymol:lactic acid 1:2, 2.5 mg of sample, 1 mL of HPB-FLUODES, and 45 min of extraction at 40°C. The LOD and LOQ were 0.09 µg g−1 and 0.29 µg g−1, respectively. The results show an enhancement of ERG fluorescence signal of 1825% against the ERG signal in methanol media. The present approach shows outstanding green performance using AGREE®, AGREEprep® and WAC principles. Finally, the analysis of edible mushrooms demonstrates the feasibility of the proposed green methodology. |
| first_indexed | 2024-03-08T11:41:13Z |
| format | Article |
| id | doaj.art-4401e93995ca4d07882900682b6ba737 |
| institution | Directory Open Access Journal |
| issn | 2772-5774 |
| language | English |
| last_indexed | 2024-03-08T11:41:13Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Green Analytical Chemistry |
| spelling | doaj.art-4401e93995ca4d07882900682b6ba7372024-01-25T05:24:12ZengElsevierGreen Analytical Chemistry2772-57742022-12-013100026Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solventRicardo Elia Dazat0Sabrina B. Mammana1Brenda V. Canizo2María Fernanda Silva3Federico J.V. Gomez4Grupo de Química Analítica Verde (GQAV), Instituto de Biología Agrícola de Mendoza (IBAM-CONICET). Facultad de Ciencias Agrarias-Universidad Nacional de Cuyo (FCA-UNCuyo). Almirante Brown 500, Chacras de Coria, Mendoza, ArgentinaGrupo de Química Analítica Verde (GQAV), Instituto de Biología Agrícola de Mendoza (IBAM-CONICET). Facultad de Ciencias Agrarias-Universidad Nacional de Cuyo (FCA-UNCuyo). Almirante Brown 500, Chacras de Coria, Mendoza, ArgentinaGrupo de Química Analítica Verde (GQAV), Instituto de Biología Agrícola de Mendoza (IBAM-CONICET). Facultad de Ciencias Agrarias-Universidad Nacional de Cuyo (FCA-UNCuyo). Almirante Brown 500, Chacras de Coria, Mendoza, ArgentinaGrupo de Química Analítica Verde (GQAV), Instituto de Biología Agrícola de Mendoza (IBAM-CONICET). Facultad de Ciencias Agrarias-Universidad Nacional de Cuyo (FCA-UNCuyo). Almirante Brown 500, Chacras de Coria, Mendoza, ArgentinaCorresponding author at: CONICET Mendoza, Argentina.; Grupo de Química Analítica Verde (GQAV), Instituto de Biología Agrícola de Mendoza (IBAM-CONICET). Facultad de Ciencias Agrarias-Universidad Nacional de Cuyo (FCA-UNCuyo). Almirante Brown 500, Chacras de Coria, Mendoza, ArgentinaErgosterol (ERG) is a sterol found at high levels in cell membranes of fungi and is a smart option to be used as proof of fungal contamination in food samples. Therefore, the objective of this work was to develop a fast, sensitive and ecological methodology based on the use of hydrophobic fluorescent natural deep eutectic solvents (HPB-FLUODES) to determine ERG by fluorescence detection in edible mushrooms. The proposed approach involves a green sample preparation and a low cost instrumental technique leading to an efficient and simple analytical methodology suitable for high-throughput analysis (2.4 samples per minute). The experimental variables, such as the type of HPB-FLUODES, sample/solvent mass/volume ratio, time, and temperature of extraction were studied and optimized. The selected variables were HPB-FLUODES thymol:lactic acid 1:2, 2.5 mg of sample, 1 mL of HPB-FLUODES, and 45 min of extraction at 40°C. The LOD and LOQ were 0.09 µg g−1 and 0.29 µg g−1, respectively. The results show an enhancement of ERG fluorescence signal of 1825% against the ERG signal in methanol media. The present approach shows outstanding green performance using AGREE®, AGREEprep® and WAC principles. Finally, the analysis of edible mushrooms demonstrates the feasibility of the proposed green methodology.http://www.sciencedirect.com/science/article/pii/S2772577422000258Enhanced fluorescenceErgosterolFoodGreen chemistryHydrophobic fluorescent natural deep eutectic solvents |
| spellingShingle | Ricardo Elia Dazat Sabrina B. Mammana Brenda V. Canizo María Fernanda Silva Federico J.V. Gomez Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent Green Analytical Chemistry Enhanced fluorescence Ergosterol Food Green chemistry Hydrophobic fluorescent natural deep eutectic solvents |
| title | Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent |
| title_full | Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent |
| title_fullStr | Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent |
| title_full_unstemmed | Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent |
| title_short | Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent |
| title_sort | enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent |
| topic | Enhanced fluorescence Ergosterol Food Green chemistry Hydrophobic fluorescent natural deep eutectic solvents |
| url | http://www.sciencedirect.com/science/article/pii/S2772577422000258 |
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