Identification of Heterodera schachtii on sugar beet in Xinjiang Uygur Autonomous Region of China
The sugar beet cyst nematode, Heterodera schachtii, is a major parasite of sugar beet which has been recognized and listed as a quarantine nematode in China and more than 20 countries and regions worldwide. A survey for important nematodes was undertaken in the sugar beet planting area of China duri...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311921637978 |
| _version_ | 1811306123194531840 |
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| author | Huan PENG Hui LIU Li GAO Ru JIANG Guang-kuo LI Hai-feng GAO Wei WU Jun WANG Yu ZHANG Wen-kun HUANG Ling-an KONG De-liang PENG |
| author_facet | Huan PENG Hui LIU Li GAO Ru JIANG Guang-kuo LI Hai-feng GAO Wei WU Jun WANG Yu ZHANG Wen-kun HUANG Ling-an KONG De-liang PENG |
| author_sort | Huan PENG |
| collection | DOAJ |
| description | The sugar beet cyst nematode, Heterodera schachtii, is a major parasite of sugar beet which has been recognized and listed as a quarantine nematode in China and more than 20 countries and regions worldwide. A survey for important nematodes was undertaken in the sugar beet planting area of China during 2015–2018, and numerous cysts were collected from sugar beet fields in Xinyuan County, Xinjiang Uygur Autonomous Region of China. The observations of morphological and morphometric characteristics revealed that cysts, vulval cones and second-stage juveniles of the Xinjiang population were in the same range of each other and within those of other reported H. schachtii populations. Molecular analysis of rDNA-ITS, 28S-D2/D3 and mtDNA cytochrome c oxidase subunit 1 (COI) gene sequences suggested that the Xinjiang population clustered in a branch with those foreign populations, and the sequence similarity was as high as 99.81–100%. Moreover, this result was confirmed by PCR assay with species-specific primer SHF6 and rDNA2 of H. schachtii, and the pathogenicity test confirmed successful Xinjiang population reproduction in both plant hosts. In conclusion, based on morphological and molecular characterization, this study confirmed that the cyst nematode population collected from sugar beet fields in Xinjiang is H. schachtii. As far as we know, this is the first report of H. schachtii on sugar beets in Xinjiang, China. |
| first_indexed | 2024-04-13T08:38:46Z |
| format | Article |
| id | doaj.art-4406f7ebedd2447c95ef49dd896fa106 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-04-13T08:38:46Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-4406f7ebedd2447c95ef49dd896fa1062022-12-22T02:53:58ZengElsevierJournal of Integrative Agriculture2095-31192022-06-0121616941702Identification of Heterodera schachtii on sugar beet in Xinjiang Uygur Autonomous Region of ChinaHuan PENG0Hui LIU1Li GAO2Ru JIANG3Guang-kuo LI4Hai-feng GAO5Wei WU6Jun WANG7Yu ZHANG8Wen-kun HUANG9Ling-an KONG10De-liang PENG11State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China; Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis of Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, P.R.ChinaThe National Agro-Tech Extension and Service Center, Beijing 100026, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Oasis of Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, P.R.ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Oasis of Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, P.R.ChinaAgricultural Technology Extension Service Center of Xinyuan County, Yining 835800, P.R.ChinaPlant Protection Station of Xinjiang Uygur Autonomous Region, Urumqi 830006, P.R.ChinaPlant Protection Station of Xinjiang Uygur Autonomous Region, Urumqi 830006, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China; Correspondence PENG De-liangThe sugar beet cyst nematode, Heterodera schachtii, is a major parasite of sugar beet which has been recognized and listed as a quarantine nematode in China and more than 20 countries and regions worldwide. A survey for important nematodes was undertaken in the sugar beet planting area of China during 2015–2018, and numerous cysts were collected from sugar beet fields in Xinyuan County, Xinjiang Uygur Autonomous Region of China. The observations of morphological and morphometric characteristics revealed that cysts, vulval cones and second-stage juveniles of the Xinjiang population were in the same range of each other and within those of other reported H. schachtii populations. Molecular analysis of rDNA-ITS, 28S-D2/D3 and mtDNA cytochrome c oxidase subunit 1 (COI) gene sequences suggested that the Xinjiang population clustered in a branch with those foreign populations, and the sequence similarity was as high as 99.81–100%. Moreover, this result was confirmed by PCR assay with species-specific primer SHF6 and rDNA2 of H. schachtii, and the pathogenicity test confirmed successful Xinjiang population reproduction in both plant hosts. In conclusion, based on morphological and molecular characterization, this study confirmed that the cyst nematode population collected from sugar beet fields in Xinjiang is H. schachtii. As far as we know, this is the first report of H. schachtii on sugar beets in Xinjiang, China.http://www.sciencedirect.com/science/article/pii/S2095311921637978sugar beet cyst nematodeHeterodera schachtiimorphologicalmolecular identification |
| spellingShingle | Huan PENG Hui LIU Li GAO Ru JIANG Guang-kuo LI Hai-feng GAO Wei WU Jun WANG Yu ZHANG Wen-kun HUANG Ling-an KONG De-liang PENG Identification of Heterodera schachtii on sugar beet in Xinjiang Uygur Autonomous Region of China Journal of Integrative Agriculture sugar beet cyst nematode Heterodera schachtii morphological molecular identification |
| title | Identification of Heterodera schachtii on sugar beet in Xinjiang Uygur Autonomous Region of China |
| title_full | Identification of Heterodera schachtii on sugar beet in Xinjiang Uygur Autonomous Region of China |
| title_fullStr | Identification of Heterodera schachtii on sugar beet in Xinjiang Uygur Autonomous Region of China |
| title_full_unstemmed | Identification of Heterodera schachtii on sugar beet in Xinjiang Uygur Autonomous Region of China |
| title_short | Identification of Heterodera schachtii on sugar beet in Xinjiang Uygur Autonomous Region of China |
| title_sort | identification of heterodera schachtii on sugar beet in xinjiang uygur autonomous region of china |
| topic | sugar beet cyst nematode Heterodera schachtii morphological molecular identification |
| url | http://www.sciencedirect.com/science/article/pii/S2095311921637978 |
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