Long-Term Effects of Magnetically Targeted Ferumoxide-Labeled Human Neural Stem Cells in Focal Cerebral Ischemia
The long-term effect of magnetically targeted neural stem cells in a rat focal cerebral ischemia model was investigated. In middle cerebral artery occlusion (MCAO) stroke model rats, ferumoxide-labeled human neural stem cells (hNSCs) were injected into the tail vein. MCAO rats were divided into thre...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2015-02-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368913X675755 |
| _version_ | 1818260682937729024 |
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| author | Miyeoun Song Young-Ju Kim Yoon-Ha Kim Jina Roh Eun-Cheol Kim Hong Jun Lee Seung U. Kim M.D., Ph.D. Byung-Woo Yoon M.D., Ph.D. |
| author_facet | Miyeoun Song Young-Ju Kim Yoon-Ha Kim Jina Roh Eun-Cheol Kim Hong Jun Lee Seung U. Kim M.D., Ph.D. Byung-Woo Yoon M.D., Ph.D. |
| author_sort | Miyeoun Song |
| collection | DOAJ |
| description | The long-term effect of magnetically targeted neural stem cells in a rat focal cerebral ischemia model was investigated. In middle cerebral artery occlusion (MCAO) stroke model rats, ferumoxide-labeled human neural stem cells (hNSCs) were injected into the tail vein. MCAO rats were divided into three groups: ischemia only (IO), ischemia with NSC injection (IC), and ischemia with NSC injection and the use of magnet targeting (IM). Four weeks after MCAO and 3 weeks posttransplantation, a greater number of hNSCs were found in ischemic lesion sites in IM rat brain compared with IO and IC animals. In addition, differentiation of hNSCs into neurons or astrocytes and angiogenesis were markedly increased. In IM rats, infarct volume was considerably reduced, and function was significantly improved. The present study indicates that long-term use of magnetic fields may be a useful way to improve the efficacy of targeted migration of stem cells and functional deficits in stem cell-based therapy for ischemic brain injury. |
| first_indexed | 2024-12-12T18:35:13Z |
| format | Article |
| id | doaj.art-4409eafef98948f4ac9f44e9c2136949 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-12T18:35:13Z |
| publishDate | 2015-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-4409eafef98948f4ac9f44e9c21369492022-12-22T00:15:47ZengSAGE PublishingCell Transplantation0963-68971555-38922015-02-012410.3727/096368913X675755Long-Term Effects of Magnetically Targeted Ferumoxide-Labeled Human Neural Stem Cells in Focal Cerebral IschemiaMiyeoun Song0Young-Ju Kim1Yoon-Ha Kim2Jina Roh3Eun-Cheol Kim4Hong Jun Lee5Seung U. Kim M.D., Ph.D.6Byung-Woo Yoon M.D., Ph.D.7 Research Center for Tooth and Periodontal Regeneration, Kyung Hee University School of Dentistry, Seoul, Korea Department of Neurology, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea Department of Neurology, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea Department of Neurology, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea Research Center for Tooth and Periodontal Regeneration, Kyung Hee University School of Dentistry, Seoul, Korea Medical Research Institute, Chung-Ang University College of Medicine, Seoul, Korea Division of Neurology, Department of Medicine, University of British Columbia, Vancouver, BC, Canada Neuroscience Research Institute, Seoul National University College of Medicine, Seoul, KoreaThe long-term effect of magnetically targeted neural stem cells in a rat focal cerebral ischemia model was investigated. In middle cerebral artery occlusion (MCAO) stroke model rats, ferumoxide-labeled human neural stem cells (hNSCs) were injected into the tail vein. MCAO rats were divided into three groups: ischemia only (IO), ischemia with NSC injection (IC), and ischemia with NSC injection and the use of magnet targeting (IM). Four weeks after MCAO and 3 weeks posttransplantation, a greater number of hNSCs were found in ischemic lesion sites in IM rat brain compared with IO and IC animals. In addition, differentiation of hNSCs into neurons or astrocytes and angiogenesis were markedly increased. In IM rats, infarct volume was considerably reduced, and function was significantly improved. The present study indicates that long-term use of magnetic fields may be a useful way to improve the efficacy of targeted migration of stem cells and functional deficits in stem cell-based therapy for ischemic brain injury.https://doi.org/10.3727/096368913X675755 |
| spellingShingle | Miyeoun Song Young-Ju Kim Yoon-Ha Kim Jina Roh Eun-Cheol Kim Hong Jun Lee Seung U. Kim M.D., Ph.D. Byung-Woo Yoon M.D., Ph.D. Long-Term Effects of Magnetically Targeted Ferumoxide-Labeled Human Neural Stem Cells in Focal Cerebral Ischemia Cell Transplantation |
| title | Long-Term Effects of Magnetically Targeted Ferumoxide-Labeled Human Neural Stem Cells in Focal Cerebral Ischemia |
| title_full | Long-Term Effects of Magnetically Targeted Ferumoxide-Labeled Human Neural Stem Cells in Focal Cerebral Ischemia |
| title_fullStr | Long-Term Effects of Magnetically Targeted Ferumoxide-Labeled Human Neural Stem Cells in Focal Cerebral Ischemia |
| title_full_unstemmed | Long-Term Effects of Magnetically Targeted Ferumoxide-Labeled Human Neural Stem Cells in Focal Cerebral Ischemia |
| title_short | Long-Term Effects of Magnetically Targeted Ferumoxide-Labeled Human Neural Stem Cells in Focal Cerebral Ischemia |
| title_sort | long term effects of magnetically targeted ferumoxide labeled human neural stem cells in focal cerebral ischemia |
| url | https://doi.org/10.3727/096368913X675755 |
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