Assessment of the Cytotoxicity, Mutagenicity, and Genotoxicity of Two Traditional Chinese Herbs: <i>Aristolochia baetica</i> and <i>Magnolia officinalis</i>
Herbal remedies used in traditional medicine often contain several compounds combined in order to potentiate their own intrinsic properties. However, herbs can sometimes cause serious health troubles. In Belgium, patients who developed severe aristolochic acid nephropathy ingested slimming pills con...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/15/1/52 |
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| author | Mélanie Poivre Marie-Hélène Antoine Kirill Kryshen Anastasia Atsapkina Alexander N. Shikov Laure Twyffels Amandine Nachtergael Pierre Duez Joëlle Nortier |
| author_facet | Mélanie Poivre Marie-Hélène Antoine Kirill Kryshen Anastasia Atsapkina Alexander N. Shikov Laure Twyffels Amandine Nachtergael Pierre Duez Joëlle Nortier |
| author_sort | Mélanie Poivre |
| collection | DOAJ |
| description | Herbal remedies used in traditional medicine often contain several compounds combined in order to potentiate their own intrinsic properties. However, herbs can sometimes cause serious health troubles. In Belgium, patients who developed severe aristolochic acid nephropathy ingested slimming pills containing root extracts of an <i>Aristolochia</i> species, as well as the bark of <i>Magnolia officinalis</i>. The goal of the study was to evaluate, on a human renal cell line, <i>Aristolochia</i> and <i>Magnolia</i> extracts for their cytotoxicity by a resazurin cell viability assay, and their genotoxicity by immunodetection and quantification of the phosphorylated histone γ-H2AX. The present study also sought to assess the mutagenicity of these extracts, employing an OECD recognized test, the Ames test, using four <i>Salmonella typhimurium</i> strains with and without a microsomial fraction. Based on our results, it has been demonstrated that the <i>Aristolochia–Magnolia</i> combination (aqueous extracts) was more genotoxic to human kidney cells, and that this combination (aqueous and methanolic extracts) was more cytotoxic to human kidney cells after 24 and 48 h. Interestingly, it has also been shown that the <i>Aristolochia–Magnolia</i> combination (aqueous extracts) was mutagenic with a TA98 <i>Salmonella typhimurium</i> strain in the presence of a microsomial liver S9 fraction. This mutagenic effect appears to be dose-dependent. |
| first_indexed | 2024-03-09T11:05:59Z |
| format | Article |
| id | doaj.art-440abeab119d441daf27d39972877e88 |
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| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T11:05:59Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-440abeab119d441daf27d39972877e882023-12-01T00:57:09ZengMDPI AGToxins2072-66512023-01-011515210.3390/toxins15010052Assessment of the Cytotoxicity, Mutagenicity, and Genotoxicity of Two Traditional Chinese Herbs: <i>Aristolochia baetica</i> and <i>Magnolia officinalis</i>Mélanie Poivre0Marie-Hélène Antoine1Kirill Kryshen2Anastasia Atsapkina3Alexander N. Shikov4Laure Twyffels5Amandine Nachtergael6Pierre Duez7Joëlle Nortier8Laboratory of Experimental Nephrology, Faculty of Medecine, Université Libre de Bruxelles, 1000 Bruxelles, BelgiumLaboratory of Experimental Nephrology, Faculty of Medecine, Université Libre de Bruxelles, 1000 Bruxelles, BelgiumSaint-Petersburg Institute of Pharmacy, 197376 Saint Petersburg, RussiaSaint-Petersburg Institute of Pharmacy, 197376 Saint Petersburg, RussiaDepartment of Technology of Pharmaceutical Formulations, St. Petersburg State Chemical Pharmaceutical University, Prof. Popov 14a, 197376 Saint Petersburg, RussiaCMMI Center for Microscopy and Molecular Imaging, 6041 Charleroi, BelgiumLaboratory of Therapeutic Chemistry and Pharmacognosy, Faculty of Medicine and Pharmacy, Research Institute for Health Sciences and Technology, University of Mons—UMONS, 7000 Mons, BelgiumLaboratory of Therapeutic Chemistry and Pharmacognosy, Faculty of Medicine and Pharmacy, Research Institute for Health Sciences and Technology, University of Mons—UMONS, 7000 Mons, BelgiumLaboratory of Experimental Nephrology, Faculty of Medecine, Université Libre de Bruxelles, 1000 Bruxelles, BelgiumHerbal remedies used in traditional medicine often contain several compounds combined in order to potentiate their own intrinsic properties. However, herbs can sometimes cause serious health troubles. In Belgium, patients who developed severe aristolochic acid nephropathy ingested slimming pills containing root extracts of an <i>Aristolochia</i> species, as well as the bark of <i>Magnolia officinalis</i>. The goal of the study was to evaluate, on a human renal cell line, <i>Aristolochia</i> and <i>Magnolia</i> extracts for their cytotoxicity by a resazurin cell viability assay, and their genotoxicity by immunodetection and quantification of the phosphorylated histone γ-H2AX. The present study also sought to assess the mutagenicity of these extracts, employing an OECD recognized test, the Ames test, using four <i>Salmonella typhimurium</i> strains with and without a microsomial fraction. Based on our results, it has been demonstrated that the <i>Aristolochia–Magnolia</i> combination (aqueous extracts) was more genotoxic to human kidney cells, and that this combination (aqueous and methanolic extracts) was more cytotoxic to human kidney cells after 24 and 48 h. Interestingly, it has also been shown that the <i>Aristolochia–Magnolia</i> combination (aqueous extracts) was mutagenic with a TA98 <i>Salmonella typhimurium</i> strain in the presence of a microsomial liver S9 fraction. This mutagenic effect appears to be dose-dependent.https://www.mdpi.com/2072-6651/15/1/52<i>Aristolochia</i><i>Magnolia</i>mutagenicitygenotoxicitycytotoxicityAmes test |
| spellingShingle | Mélanie Poivre Marie-Hélène Antoine Kirill Kryshen Anastasia Atsapkina Alexander N. Shikov Laure Twyffels Amandine Nachtergael Pierre Duez Joëlle Nortier Assessment of the Cytotoxicity, Mutagenicity, and Genotoxicity of Two Traditional Chinese Herbs: <i>Aristolochia baetica</i> and <i>Magnolia officinalis</i> Toxins <i>Aristolochia</i> <i>Magnolia</i> mutagenicity genotoxicity cytotoxicity Ames test |
| title | Assessment of the Cytotoxicity, Mutagenicity, and Genotoxicity of Two Traditional Chinese Herbs: <i>Aristolochia baetica</i> and <i>Magnolia officinalis</i> |
| title_full | Assessment of the Cytotoxicity, Mutagenicity, and Genotoxicity of Two Traditional Chinese Herbs: <i>Aristolochia baetica</i> and <i>Magnolia officinalis</i> |
| title_fullStr | Assessment of the Cytotoxicity, Mutagenicity, and Genotoxicity of Two Traditional Chinese Herbs: <i>Aristolochia baetica</i> and <i>Magnolia officinalis</i> |
| title_full_unstemmed | Assessment of the Cytotoxicity, Mutagenicity, and Genotoxicity of Two Traditional Chinese Herbs: <i>Aristolochia baetica</i> and <i>Magnolia officinalis</i> |
| title_short | Assessment of the Cytotoxicity, Mutagenicity, and Genotoxicity of Two Traditional Chinese Herbs: <i>Aristolochia baetica</i> and <i>Magnolia officinalis</i> |
| title_sort | assessment of the cytotoxicity mutagenicity and genotoxicity of two traditional chinese herbs i aristolochia baetica i and i magnolia officinalis i |
| topic | <i>Aristolochia</i> <i>Magnolia</i> mutagenicity genotoxicity cytotoxicity Ames test |
| url | https://www.mdpi.com/2072-6651/15/1/52 |
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