Investigation on Brucella infection in farm animals in Saham, Sultanate of Oman with reference to human brucellosis outbreak
Abstract Background The objective of this study was to investigate Brucella infection in farm animals in Saham, Oman, with reference to a survey carried out by the Ministry of Agriculture & Fisheries (MAF) for Brucellosis during the period of May to July 2016 in Saham, following an outbreak of h...
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| Format: | Article |
| Language: | English |
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BMC
2019-10-01
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| Series: | BMC Veterinary Research |
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| Online Access: | http://link.springer.com/article/10.1186/s12917-019-2093-4 |
| _version_ | 1811222212230774784 |
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| author | Yasmin ElTahir Anfal Al-Farsi Waleed Al-Marzooqi Alghalya Al-Toobi Osman M. Gaafar Maryne Jay Yannick Corde Shekar Bose Abeer Al-Hamrashdi Kaadhia Al-Kharousi Sunil Rajamony Muhammed Nadeem Asi Nasseb Al-Saqri Rudaina AlBusaidi Elshafie I Elshafie Eugene H. Johnson |
| author_facet | Yasmin ElTahir Anfal Al-Farsi Waleed Al-Marzooqi Alghalya Al-Toobi Osman M. Gaafar Maryne Jay Yannick Corde Shekar Bose Abeer Al-Hamrashdi Kaadhia Al-Kharousi Sunil Rajamony Muhammed Nadeem Asi Nasseb Al-Saqri Rudaina AlBusaidi Elshafie I Elshafie Eugene H. Johnson |
| author_sort | Yasmin ElTahir |
| collection | DOAJ |
| description | Abstract Background The objective of this study was to investigate Brucella infection in farm animals in Saham, Oman, with reference to a survey carried out by the Ministry of Agriculture & Fisheries (MAF) for Brucellosis during the period of May to July 2016 in Saham, following an outbreak of human brucellosis. We wanted to apply different serological, bacteriological and molecular tests in a time frame (phase 1, 2 & 3) with reference to the pivotal time of a human brucellosis outbreak to ascertain the status of the disease in Saham area where the MAF survey was conducted. Blood samples were collected from farm animals and sera were screened in parallel for Brucella antibodies using different serological tests. Results Using the RBT test, phase 1 sera showed seropositivity in sheep at 2.6%, (95% CI: 0.5–13.5%), in camel (5.9%, 1.1–27.0%), but not in sera from goats and cattle (0%). Using I-ELISA, seropositivity in goat was 3.1% (0.6–15.8%), with no positive sheep and cattle. Using c-ELISA for camel we found a seropositivity of 5.9% (1.1–27.0%). Furthermore, CFT seropositivity in goats was 21.9% (CI: 11.3–38.9), cattle and sheep sera were negative and camel was 5.9% (1.1–27.0%). In phase 2, the seropositivity in goats was 1.9% (1.4–2.6%), sheep 4.5% (3.5–5.8%), cattle 1.1%, (0.5–2.3%) and camels 18.2% (5.1–47.7%), Phase 3 sera were collected 6 months after the human brucellosis outbreak. With RBT, the seropositivity in goats was 3% (1.0–8.5%), sheep 2% (0.6–7.1%) cattle 1% (0.2–5.5%). With I-ELISA, goats & camels were negative, sheep were 3% (1.0–8.5%) and cattle 1% (0.2–5.5%). Moreover, B. melitensis was isolated from a bronchial lymph node of the RBT and I-ELISA seropositive cow and confirmed by Multiplex PCR and biochemical tests. Conclusion Using a retrospective study analysis of animal sera and following up after a human brucellosis outbreak, the present study showed a slight decrease in seropositivity of infected animals after the MAF implemented test and slaughter policy. The most interesting finding in this study was the isolation, identification and molecular characterization of Brucella melitensis in a cow (spillover), which is not a preferential host for Brucella melitensis. |
| first_indexed | 2024-04-12T08:12:09Z |
| format | Article |
| id | doaj.art-440cf16d645e4279bd22a079207692fe |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-12T08:12:09Z |
| publishDate | 2019-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-440cf16d645e4279bd22a079207692fe2022-12-22T03:40:56ZengBMCBMC Veterinary Research1746-61482019-10-011511810.1186/s12917-019-2093-4Investigation on Brucella infection in farm animals in Saham, Sultanate of Oman with reference to human brucellosis outbreakYasmin ElTahir0Anfal Al-Farsi1Waleed Al-Marzooqi2Alghalya Al-Toobi3Osman M. Gaafar4Maryne Jay5Yannick Corde6Shekar Bose7Abeer Al-Hamrashdi8Kaadhia Al-Kharousi9Sunil Rajamony10Muhammed Nadeem Asi11Nasseb Al-Saqri12Rudaina AlBusaidi13Elshafie I Elshafie14Eugene H. Johnson15Department of Animal & Veterinary Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos UniversityDepartment of Animal & Veterinary Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos UniversityDepartment of Animal & Veterinary Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos UniversityDepartment of Animal & Veterinary Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos UniversityDepartment of Animal & Veterinary Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos UniversityParis-Est University/Anses, EU/OIE/FAO & National Reference Laboratory for Brucellosis, Animal Health LaboratoryParis-Est University/Anses, EU/OIE/FAO & National Reference Laboratory for Brucellosis, Animal Health LaboratoryDepartment of Natural Resources Economics, Sultan Qaboos UniversityDepartment of Animal & Veterinary Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos UniversityDepartment of Animal & Veterinary Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos UniversityDepartment of Animal & Veterinary Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos UniversityDepartment of Animal & Veterinary Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos UniversityDepartment of Animal & Veterinary Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos UniversityDepartment of Animal & Veterinary Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos UniversityDepartment of Animal & Veterinary Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos UniversityDepartment of Animal & Veterinary Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos UniversityAbstract Background The objective of this study was to investigate Brucella infection in farm animals in Saham, Oman, with reference to a survey carried out by the Ministry of Agriculture & Fisheries (MAF) for Brucellosis during the period of May to July 2016 in Saham, following an outbreak of human brucellosis. We wanted to apply different serological, bacteriological and molecular tests in a time frame (phase 1, 2 & 3) with reference to the pivotal time of a human brucellosis outbreak to ascertain the status of the disease in Saham area where the MAF survey was conducted. Blood samples were collected from farm animals and sera were screened in parallel for Brucella antibodies using different serological tests. Results Using the RBT test, phase 1 sera showed seropositivity in sheep at 2.6%, (95% CI: 0.5–13.5%), in camel (5.9%, 1.1–27.0%), but not in sera from goats and cattle (0%). Using I-ELISA, seropositivity in goat was 3.1% (0.6–15.8%), with no positive sheep and cattle. Using c-ELISA for camel we found a seropositivity of 5.9% (1.1–27.0%). Furthermore, CFT seropositivity in goats was 21.9% (CI: 11.3–38.9), cattle and sheep sera were negative and camel was 5.9% (1.1–27.0%). In phase 2, the seropositivity in goats was 1.9% (1.4–2.6%), sheep 4.5% (3.5–5.8%), cattle 1.1%, (0.5–2.3%) and camels 18.2% (5.1–47.7%), Phase 3 sera were collected 6 months after the human brucellosis outbreak. With RBT, the seropositivity in goats was 3% (1.0–8.5%), sheep 2% (0.6–7.1%) cattle 1% (0.2–5.5%). With I-ELISA, goats & camels were negative, sheep were 3% (1.0–8.5%) and cattle 1% (0.2–5.5%). Moreover, B. melitensis was isolated from a bronchial lymph node of the RBT and I-ELISA seropositive cow and confirmed by Multiplex PCR and biochemical tests. Conclusion Using a retrospective study analysis of animal sera and following up after a human brucellosis outbreak, the present study showed a slight decrease in seropositivity of infected animals after the MAF implemented test and slaughter policy. The most interesting finding in this study was the isolation, identification and molecular characterization of Brucella melitensis in a cow (spillover), which is not a preferential host for Brucella melitensis.http://link.springer.com/article/10.1186/s12917-019-2093-4BrucellosisBrucella melitensisSahamOman |
| spellingShingle | Yasmin ElTahir Anfal Al-Farsi Waleed Al-Marzooqi Alghalya Al-Toobi Osman M. Gaafar Maryne Jay Yannick Corde Shekar Bose Abeer Al-Hamrashdi Kaadhia Al-Kharousi Sunil Rajamony Muhammed Nadeem Asi Nasseb Al-Saqri Rudaina AlBusaidi Elshafie I Elshafie Eugene H. Johnson Investigation on Brucella infection in farm animals in Saham, Sultanate of Oman with reference to human brucellosis outbreak BMC Veterinary Research Brucellosis Brucella melitensis Saham Oman |
| title | Investigation on Brucella infection in farm animals in Saham, Sultanate of Oman with reference to human brucellosis outbreak |
| title_full | Investigation on Brucella infection in farm animals in Saham, Sultanate of Oman with reference to human brucellosis outbreak |
| title_fullStr | Investigation on Brucella infection in farm animals in Saham, Sultanate of Oman with reference to human brucellosis outbreak |
| title_full_unstemmed | Investigation on Brucella infection in farm animals in Saham, Sultanate of Oman with reference to human brucellosis outbreak |
| title_short | Investigation on Brucella infection in farm animals in Saham, Sultanate of Oman with reference to human brucellosis outbreak |
| title_sort | investigation on brucella infection in farm animals in saham sultanate of oman with reference to human brucellosis outbreak |
| topic | Brucellosis Brucella melitensis Saham Oman |
| url | http://link.springer.com/article/10.1186/s12917-019-2093-4 |
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