Design of stable magnetic hybrid nanoparticles of Si-entrapped HRP.
Hybrid and composite nanoparticles represent an attractive material for enzyme integration due to possible synergic advantages of the structural builders in the properties of the nanobiocatalyst. In this study, we report the synthesis of a new stable hybrid nanobiocatalyst formed by biomimetic silic...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0214004 |
| _version_ | 1819114876791947264 |
|---|---|
| author | Sonali Correa Sara Puertas Lucía Gutiérrez Laura Asín Jesús Martínez de la Fuente Valeria Grazú Lorena Betancor |
| author_facet | Sonali Correa Sara Puertas Lucía Gutiérrez Laura Asín Jesús Martínez de la Fuente Valeria Grazú Lorena Betancor |
| author_sort | Sonali Correa |
| collection | DOAJ |
| description | Hybrid and composite nanoparticles represent an attractive material for enzyme integration due to possible synergic advantages of the structural builders in the properties of the nanobiocatalyst. In this study, we report the synthesis of a new stable hybrid nanobiocatalyst formed by biomimetic silica (Si) nanoparticles entrapping both Horseradish Peroxidase (HRP) (EC 1.11.1.7) and magnetic nanoparticles (MNPs). We have demonstrated that tailoring of the synthetic reagents and post immobilization treatments greatly impacted physical and biocatalytic properties such as an unprecedented ~280 times increase in the half-life time in thermal stability experiments. The optimized nanohybrid biocatalyst that showed superparamagnetic behaviour, was effective in the batch conversion of indole-3-acetic acid, a prodrug used in Direct Enzyme Prodrug Therapy (DEPT). Our system, that was not cytotoxic per se, showed enhanced cytotoxic activity in the presence of the prodrug towards HCT-116, a colorectal cancer cell line. The strategy developed proved to be effective in obtaining a stabilized nanobiocatalyst combining three different organic/inorganic materials with potential in DEPT and other biotechnological applications. |
| first_indexed | 2024-12-22T04:52:16Z |
| format | Article |
| id | doaj.art-441493d1195a4f5c8b65b28139f335a4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T04:52:16Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-441493d1195a4f5c8b65b28139f335a42022-12-21T18:38:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01144e021400410.1371/journal.pone.0214004Design of stable magnetic hybrid nanoparticles of Si-entrapped HRP.Sonali CorreaSara PuertasLucía GutiérrezLaura AsínJesús Martínez de la FuenteValeria GrazúLorena BetancorHybrid and composite nanoparticles represent an attractive material for enzyme integration due to possible synergic advantages of the structural builders in the properties of the nanobiocatalyst. In this study, we report the synthesis of a new stable hybrid nanobiocatalyst formed by biomimetic silica (Si) nanoparticles entrapping both Horseradish Peroxidase (HRP) (EC 1.11.1.7) and magnetic nanoparticles (MNPs). We have demonstrated that tailoring of the synthetic reagents and post immobilization treatments greatly impacted physical and biocatalytic properties such as an unprecedented ~280 times increase in the half-life time in thermal stability experiments. The optimized nanohybrid biocatalyst that showed superparamagnetic behaviour, was effective in the batch conversion of indole-3-acetic acid, a prodrug used in Direct Enzyme Prodrug Therapy (DEPT). Our system, that was not cytotoxic per se, showed enhanced cytotoxic activity in the presence of the prodrug towards HCT-116, a colorectal cancer cell line. The strategy developed proved to be effective in obtaining a stabilized nanobiocatalyst combining three different organic/inorganic materials with potential in DEPT and other biotechnological applications.https://doi.org/10.1371/journal.pone.0214004 |
| spellingShingle | Sonali Correa Sara Puertas Lucía Gutiérrez Laura Asín Jesús Martínez de la Fuente Valeria Grazú Lorena Betancor Design of stable magnetic hybrid nanoparticles of Si-entrapped HRP. PLoS ONE |
| title | Design of stable magnetic hybrid nanoparticles of Si-entrapped HRP. |
| title_full | Design of stable magnetic hybrid nanoparticles of Si-entrapped HRP. |
| title_fullStr | Design of stable magnetic hybrid nanoparticles of Si-entrapped HRP. |
| title_full_unstemmed | Design of stable magnetic hybrid nanoparticles of Si-entrapped HRP. |
| title_short | Design of stable magnetic hybrid nanoparticles of Si-entrapped HRP. |
| title_sort | design of stable magnetic hybrid nanoparticles of si entrapped hrp |
| url | https://doi.org/10.1371/journal.pone.0214004 |
| work_keys_str_mv | AT sonalicorrea designofstablemagnetichybridnanoparticlesofsientrappedhrp AT sarapuertas designofstablemagnetichybridnanoparticlesofsientrappedhrp AT luciagutierrez designofstablemagnetichybridnanoparticlesofsientrappedhrp AT lauraasin designofstablemagnetichybridnanoparticlesofsientrappedhrp AT jesusmartinezdelafuente designofstablemagnetichybridnanoparticlesofsientrappedhrp AT valeriagrazu designofstablemagnetichybridnanoparticlesofsientrappedhrp AT lorenabetancor designofstablemagnetichybridnanoparticlesofsientrappedhrp |