MiR-15a Decreases Bovine Mammary Epithelial Cell Viability and Lactation and Regulates Growth Hormone Receptor Expression
MicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate the expression of target genes at the post-transcriptional level by transcript degradation or translational inhibition. The role of bta-miR-15a in bovine mammary gland hasn’t been reported. Using miRNAs prediction software, GHR ge...
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| Format: | Article |
| Language: | English |
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MDPI AG
2012-10-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/10/12037 |
| _version_ | 1818025079527702528 |
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| author | Xue-Jun Gao Qing-Zhang Li Chun-Mei Wang Hui-Ming Li |
| author_facet | Xue-Jun Gao Qing-Zhang Li Chun-Mei Wang Hui-Ming Li |
| author_sort | Xue-Jun Gao |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate the expression of target genes at the post-transcriptional level by transcript degradation or translational inhibition. The role of bta-miR-15a in bovine mammary gland hasn’t been reported. Using miRNAs prediction software, GHR gene was predicted to be a potential target of bta-miR-15a. In this study, bovine mammary epithelial cell line was used as an in vitro cell model to address the function of bta-miR-15a on bovine mammary epithelial cells. The expression changes of bta-miR-15a and Ghr after bta-miR-15a transfection were detected by qRT-PCR; the expression of GHR protein and casein was detected by western blotting. To determine whether bta-miR-15a can affect cell viability, cells were examined using an electronic Coulter counter (CASY-TT). In conclusion, bta-miR-15a inhibited the expression of casein of bovine mammary epithelial cells, and cell number and viability were reduced by bta-miR-15a expression. Bta-miR-15a inhibited the viability of mammary epithelial cells as well as the expression of GHR mRNA and protein level, therefore suggesting that bta-miR-15a may play an important role in mammary gland physiology. |
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| format | Article |
| id | doaj.art-4414942fc172421698ba7be8686dbc66 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T04:10:24Z |
| publishDate | 2012-10-01 |
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| spelling | doaj.art-4414942fc172421698ba7be8686dbc662022-12-22T02:02:45ZengMDPI AGMolecules1420-30492012-10-011710120371204810.3390/molecules171012037MiR-15a Decreases Bovine Mammary Epithelial Cell Viability and Lactation and Regulates Growth Hormone Receptor ExpressionXue-Jun GaoQing-Zhang LiChun-Mei WangHui-Ming LiMicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate the expression of target genes at the post-transcriptional level by transcript degradation or translational inhibition. The role of bta-miR-15a in bovine mammary gland hasn’t been reported. Using miRNAs prediction software, GHR gene was predicted to be a potential target of bta-miR-15a. In this study, bovine mammary epithelial cell line was used as an in vitro cell model to address the function of bta-miR-15a on bovine mammary epithelial cells. The expression changes of bta-miR-15a and Ghr after bta-miR-15a transfection were detected by qRT-PCR; the expression of GHR protein and casein was detected by western blotting. To determine whether bta-miR-15a can affect cell viability, cells were examined using an electronic Coulter counter (CASY-TT). In conclusion, bta-miR-15a inhibited the expression of casein of bovine mammary epithelial cells, and cell number and viability were reduced by bta-miR-15a expression. Bta-miR-15a inhibited the viability of mammary epithelial cells as well as the expression of GHR mRNA and protein level, therefore suggesting that bta-miR-15a may play an important role in mammary gland physiology.http://www.mdpi.com/1420-3049/17/10/12037bta-miR-15aGhrproliferationbovine mammary epithelial cell |
| spellingShingle | Xue-Jun Gao Qing-Zhang Li Chun-Mei Wang Hui-Ming Li MiR-15a Decreases Bovine Mammary Epithelial Cell Viability and Lactation and Regulates Growth Hormone Receptor Expression Molecules bta-miR-15a Ghr proliferation bovine mammary epithelial cell |
| title | MiR-15a Decreases Bovine Mammary Epithelial Cell Viability and Lactation and Regulates Growth Hormone Receptor Expression |
| title_full | MiR-15a Decreases Bovine Mammary Epithelial Cell Viability and Lactation and Regulates Growth Hormone Receptor Expression |
| title_fullStr | MiR-15a Decreases Bovine Mammary Epithelial Cell Viability and Lactation and Regulates Growth Hormone Receptor Expression |
| title_full_unstemmed | MiR-15a Decreases Bovine Mammary Epithelial Cell Viability and Lactation and Regulates Growth Hormone Receptor Expression |
| title_short | MiR-15a Decreases Bovine Mammary Epithelial Cell Viability and Lactation and Regulates Growth Hormone Receptor Expression |
| title_sort | mir 15a decreases bovine mammary epithelial cell viability and lactation and regulates growth hormone receptor expression |
| topic | bta-miR-15a Ghr proliferation bovine mammary epithelial cell |
| url | http://www.mdpi.com/1420-3049/17/10/12037 |
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