Untargeted polysulfide omics analysis of alternations in polysulfide production during the germination of broccoli sprouts
Higher consumption of broccoli (Brassica oleracea var. italica) is associated with a reduced risk of cardiometabolic diseases, neurological disorders, diabetes, and cancer. Broccoli is rich in various phytochemicals, including glucosinolates, and isothiocyanates. Moreover, it has recently reported t...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Redox Biology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213231723002768 |
| _version_ | 1797647880323858432 |
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| author | Shingo Kasamatsu Takuma Owaki Somei Komae Ayaka Kinno Tomoaki Ida Takaaki Akaike Hideshi Ihara |
| author_facet | Shingo Kasamatsu Takuma Owaki Somei Komae Ayaka Kinno Tomoaki Ida Takaaki Akaike Hideshi Ihara |
| author_sort | Shingo Kasamatsu |
| collection | DOAJ |
| description | Higher consumption of broccoli (Brassica oleracea var. italica) is associated with a reduced risk of cardiometabolic diseases, neurological disorders, diabetes, and cancer. Broccoli is rich in various phytochemicals, including glucosinolates, and isothiocyanates. Moreover, it has recently reported the endogenous production of polysulfides, such as cysteine hydropersulfide (CysS2H) and glutathione hydropersulfide (GS2H), in mammals including humans, and that these bioactive substances function as potent antioxidants and important regulators of redox signaling in vivo. However, few studies have focused on the endogenous polysulfide content of broccoli and the impact of germination on the polysulfide content and composition in broccoli. In this study, we investigated the alternations in polysulfide biosynthesis in broccoli during germination by performing untargeted polysulfide omics analysis and quantitative targeted polysulfide metabolomics through liquid chromatography–electrospray ionization–tandem mass spectrometry. We also performed 2,2-diphenyl-1-picrylhydrazyl radical–scavenging assay to determine the antioxidant properties of the polysulfides. The results revealed that the total polysulfide content of broccoli sprouts significantly increased during germination and growth; CysS2H and cysteine hydrotrisulfide were the predominant organic polysulfide metabolites. Furthermore, we determined that novel sulforaphane (SFN) derivatives conjugated with CysS2H and GS2H were endogenously produced in the broccoli sprouts, and the novel SFN conjugated with CysS2H exhibited a greater radical scavenging capacity than SFN and cysteine. These results suggest that the abundance of polysulfides in broccoli sprouts contribute to their health-promoting properties. Our findings have important biological implications for the development of novel pharmacological targets for the health-promoting effects of broccoli sprouts in humans. |
| first_indexed | 2024-03-11T15:24:07Z |
| format | Article |
| id | doaj.art-44174cd3e7a14db9bd5f9079f6689077 |
| institution | Directory Open Access Journal |
| issn | 2213-2317 |
| language | English |
| last_indexed | 2024-03-11T15:24:07Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Redox Biology |
| spelling | doaj.art-44174cd3e7a14db9bd5f9079f66890772023-10-28T05:07:08ZengElsevierRedox Biology2213-23172023-11-0167102875Untargeted polysulfide omics analysis of alternations in polysulfide production during the germination of broccoli sproutsShingo Kasamatsu0Takuma Owaki1Somei Komae2Ayaka Kinno3Tomoaki Ida4Takaaki Akaike5Hideshi Ihara6Department of Biological Chemistry, Graduate School of Science, Osaka Metropolitan University, Sakai, 599-8531, Japan; Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Sakai, 599-8531, JapanDepartment of Biological Chemistry, Graduate School of Science, Osaka Metropolitan University, Sakai, 599-8531, JapanDepartment of Biological Science, Graduate School of Science, Osaka Prefecture University, Sakai, 599-8531, JapanDepartment of Biological Science, Graduate School of Science, Osaka Prefecture University, Sakai, 599-8531, JapanOrganization for Research Promotion, Osaka Metropolitan University, Sakai, 599-8531, JapanDepartment of Environmental Medicine and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai, Miyagi, 980-8575, JapanDepartment of Biological Chemistry, Graduate School of Science, Osaka Metropolitan University, Sakai, 599-8531, Japan; Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Sakai, 599-8531, Japan; Corresponding author. Department of Biological Chemistry, Graduate School of Science, Osaka Metropolitan University, Sakai 599-8531, Japan.Higher consumption of broccoli (Brassica oleracea var. italica) is associated with a reduced risk of cardiometabolic diseases, neurological disorders, diabetes, and cancer. Broccoli is rich in various phytochemicals, including glucosinolates, and isothiocyanates. Moreover, it has recently reported the endogenous production of polysulfides, such as cysteine hydropersulfide (CysS2H) and glutathione hydropersulfide (GS2H), in mammals including humans, and that these bioactive substances function as potent antioxidants and important regulators of redox signaling in vivo. However, few studies have focused on the endogenous polysulfide content of broccoli and the impact of germination on the polysulfide content and composition in broccoli. In this study, we investigated the alternations in polysulfide biosynthesis in broccoli during germination by performing untargeted polysulfide omics analysis and quantitative targeted polysulfide metabolomics through liquid chromatography–electrospray ionization–tandem mass spectrometry. We also performed 2,2-diphenyl-1-picrylhydrazyl radical–scavenging assay to determine the antioxidant properties of the polysulfides. The results revealed that the total polysulfide content of broccoli sprouts significantly increased during germination and growth; CysS2H and cysteine hydrotrisulfide were the predominant organic polysulfide metabolites. Furthermore, we determined that novel sulforaphane (SFN) derivatives conjugated with CysS2H and GS2H were endogenously produced in the broccoli sprouts, and the novel SFN conjugated with CysS2H exhibited a greater radical scavenging capacity than SFN and cysteine. These results suggest that the abundance of polysulfides in broccoli sprouts contribute to their health-promoting properties. Our findings have important biological implications for the development of novel pharmacological targets for the health-promoting effects of broccoli sprouts in humans.http://www.sciencedirect.com/science/article/pii/S2213231723002768PolysulfideBroccoli sproutsCysteine hydrotrisulfideSulforaphaneAntioxidant activity |
| spellingShingle | Shingo Kasamatsu Takuma Owaki Somei Komae Ayaka Kinno Tomoaki Ida Takaaki Akaike Hideshi Ihara Untargeted polysulfide omics analysis of alternations in polysulfide production during the germination of broccoli sprouts Redox Biology Polysulfide Broccoli sprouts Cysteine hydrotrisulfide Sulforaphane Antioxidant activity |
| title | Untargeted polysulfide omics analysis of alternations in polysulfide production during the germination of broccoli sprouts |
| title_full | Untargeted polysulfide omics analysis of alternations in polysulfide production during the germination of broccoli sprouts |
| title_fullStr | Untargeted polysulfide omics analysis of alternations in polysulfide production during the germination of broccoli sprouts |
| title_full_unstemmed | Untargeted polysulfide omics analysis of alternations in polysulfide production during the germination of broccoli sprouts |
| title_short | Untargeted polysulfide omics analysis of alternations in polysulfide production during the germination of broccoli sprouts |
| title_sort | untargeted polysulfide omics analysis of alternations in polysulfide production during the germination of broccoli sprouts |
| topic | Polysulfide Broccoli sprouts Cysteine hydrotrisulfide Sulforaphane Antioxidant activity |
| url | http://www.sciencedirect.com/science/article/pii/S2213231723002768 |
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