pNiPAM-Nanoparticle-Based Antiapoptotic Approach for Pro-Regenerative Capacity of Skeletal Myogenic Cells
The biocompatibility of pNiPAM (Poly N-isopropylacrylamide) copolymers has been examined and they did not exert any cytotoxic effects. Their properties and vulnerable temperature characteristics make them candidates for use in medical applications. We synthesized a well-characterized nanoparticles-b...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/10/2495 |
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| author | Magdalena Nowaczyk Agnieszka Zimna Tobiasz Deptuła Katarzyna Fiedorowicz Natalia Rozwadowska Marta Podralska Maciej Kurpisz |
| author_facet | Magdalena Nowaczyk Agnieszka Zimna Tobiasz Deptuła Katarzyna Fiedorowicz Natalia Rozwadowska Marta Podralska Maciej Kurpisz |
| author_sort | Magdalena Nowaczyk |
| collection | DOAJ |
| description | The biocompatibility of pNiPAM (Poly N-isopropylacrylamide) copolymers has been examined and they did not exert any cytotoxic effects. Their properties and vulnerable temperature characteristics make them candidates for use in medical applications. We synthesized a well-characterized nanoparticles-based cargo system that would effectively deliver a biological agent to human skeletal myogenic cells (SkMCs); among other aspects, a downregulating apoptotic pathway potentially responsible for poor regeneration of myocardium. We confirmed the size of the pNiPAM based spheres at around 100 nm and the nanomeric shape of nanoparticles (NP) obtained. We confirmed that 33 °C is the adequate temperature for phase transition. We performed the dynamics of cargo release. A small amount of examined protein was detected at 10 min after reaching LCTS (lower critical solution temperature). The presented results of the test with BSA (bovine serum albumin) and doxorubicin loaded into nanoparticles showed a similar release profile for both substances. SkMCs incubated with NP loaded with antiapoptotic agent, BCB (Bax channel blocker), significantly diminished cell apoptosis (<i>p</i> < 0.01). Moreover, the lowest apoptotic level was detected in SkMCs treated with camptothecin and simultaneously incubated with pNiPAMs loaded with BCB. Application of nanoparticles loaded with BCB or subjected to BCB alone did not, however, diminish the amount of apparently necrotic cells. |
| first_indexed | 2024-03-10T06:20:00Z |
| format | Article |
| id | doaj.art-4418787b688c427c94ccd9d4e5c8827a |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T06:20:00Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-4418787b688c427c94ccd9d4e5c8827a2023-11-22T19:22:08ZengMDPI AGNanomaterials2079-49912021-09-011110249510.3390/nano11102495pNiPAM-Nanoparticle-Based Antiapoptotic Approach for Pro-Regenerative Capacity of Skeletal Myogenic CellsMagdalena Nowaczyk0Agnieszka Zimna1Tobiasz Deptuła2Katarzyna Fiedorowicz3Natalia Rozwadowska4Marta Podralska5Maciej Kurpisz6Institute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandInstitute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandInstitute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandInstitute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandInstitute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandInstitute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandInstitute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandThe biocompatibility of pNiPAM (Poly N-isopropylacrylamide) copolymers has been examined and they did not exert any cytotoxic effects. Their properties and vulnerable temperature characteristics make them candidates for use in medical applications. We synthesized a well-characterized nanoparticles-based cargo system that would effectively deliver a biological agent to human skeletal myogenic cells (SkMCs); among other aspects, a downregulating apoptotic pathway potentially responsible for poor regeneration of myocardium. We confirmed the size of the pNiPAM based spheres at around 100 nm and the nanomeric shape of nanoparticles (NP) obtained. We confirmed that 33 °C is the adequate temperature for phase transition. We performed the dynamics of cargo release. A small amount of examined protein was detected at 10 min after reaching LCTS (lower critical solution temperature). The presented results of the test with BSA (bovine serum albumin) and doxorubicin loaded into nanoparticles showed a similar release profile for both substances. SkMCs incubated with NP loaded with antiapoptotic agent, BCB (Bax channel blocker), significantly diminished cell apoptosis (<i>p</i> < 0.01). Moreover, the lowest apoptotic level was detected in SkMCs treated with camptothecin and simultaneously incubated with pNiPAMs loaded with BCB. Application of nanoparticles loaded with BCB or subjected to BCB alone did not, however, diminish the amount of apparently necrotic cells.https://www.mdpi.com/2079-4991/11/10/2495nanoparticlespNiPAMapoptosisnecrosisbax channel blockerskeletal muscle derived stem/progenitor cells |
| spellingShingle | Magdalena Nowaczyk Agnieszka Zimna Tobiasz Deptuła Katarzyna Fiedorowicz Natalia Rozwadowska Marta Podralska Maciej Kurpisz pNiPAM-Nanoparticle-Based Antiapoptotic Approach for Pro-Regenerative Capacity of Skeletal Myogenic Cells Nanomaterials nanoparticles pNiPAM apoptosis necrosis bax channel blocker skeletal muscle derived stem/progenitor cells |
| title | pNiPAM-Nanoparticle-Based Antiapoptotic Approach for Pro-Regenerative Capacity of Skeletal Myogenic Cells |
| title_full | pNiPAM-Nanoparticle-Based Antiapoptotic Approach for Pro-Regenerative Capacity of Skeletal Myogenic Cells |
| title_fullStr | pNiPAM-Nanoparticle-Based Antiapoptotic Approach for Pro-Regenerative Capacity of Skeletal Myogenic Cells |
| title_full_unstemmed | pNiPAM-Nanoparticle-Based Antiapoptotic Approach for Pro-Regenerative Capacity of Skeletal Myogenic Cells |
| title_short | pNiPAM-Nanoparticle-Based Antiapoptotic Approach for Pro-Regenerative Capacity of Skeletal Myogenic Cells |
| title_sort | pnipam nanoparticle based antiapoptotic approach for pro regenerative capacity of skeletal myogenic cells |
| topic | nanoparticles pNiPAM apoptosis necrosis bax channel blocker skeletal muscle derived stem/progenitor cells |
| url | https://www.mdpi.com/2079-4991/11/10/2495 |
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