Deregulation of Cancer-Associated Genes in Odontogenic Cysts
Objectives: The aim of the present study was to demonstrate the key role of differential expression levels of RB1, TP53, XIAP, BCL2 AIFM3, BAX, CASP3 and CASP9 genes in odontogenic cysts. Materials and Methods: A total number of 15 patients who diagnosed with odontogenic cyst were enrolled for the p...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Cumhuriyet University
2019-09-01
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| Series: | Cumhuriyet Dental Journal |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/download/article-file/803479 |
| Summary: | Objectives: The
aim of the present study was to demonstrate the key role of differential
expression levels of RB1, TP53, XIAP, BCL2 AIFM3, BAX, CASP3 and CASP9 genes in
odontogenic cysts. Materials and Methods:
A total number of 15 patients who diagnosed with odontogenic cyst were enrolled
for the present study. For the quantitative gene expression analysis, cyst and
adjacent gingival healthy tissues of patients were collected during surgical assessments.
Quantitative analysis of gene expression levels RB1, TP53, XIAP, BCL2 AIFM3,
BAX, CASP3 and CASP9 were achieved real-time PCR method. For the optimization
of gene expression levels GAPDH reference gene was used. Results:
Expression of both RB1 and TP53 genes were markedly diminished in odontogenic
cysts tissues as compared to healthy tissues (p<0.05). Likewise, levels of
CASP3 and CASP9 genes were found to be significantly reduced in odontogenic
cysts tissues compared to healthy tissues (p<0.05). In contrast, expression levels
of XIAP was significantly elevated (p<0.05). Although BCL2, AIFM3, and BAX
genes were also differentially expressed in odontogenic cysts tissues, these variations
were statistically insignificant (p>0.05).
Conclusions:
The findings of the present study indicates that RB1, TP53, XIAP, CASP3 and
CASP9 genes might have chief roles in formation odontogenic cysts and
responsible for the increased cell proliferation in these tissues. |
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| ISSN: | 1302-5805 2146-2852 |