Deregulation of Cancer-Associated Genes in Odontogenic Cysts
Objectives: The aim of the present study was to demonstrate the key role of differential expression levels of RB1, TP53, XIAP, BCL2 AIFM3, BAX, CASP3 and CASP9 genes in odontogenic cysts. Materials and Methods: A total number of 15 patients who diagnosed with odontogenic cyst were enrolled for the p...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Cumhuriyet University
2019-09-01
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| Series: | Cumhuriyet Dental Journal |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/download/article-file/803479 |
| _version_ | 1797703782242451456 |
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| author | İbrahim Bozgeyik Önder Yumrutaş Bilal Ege |
| author_facet | İbrahim Bozgeyik Önder Yumrutaş Bilal Ege |
| author_sort | İbrahim Bozgeyik |
| collection | DOAJ |
| description | Objectives: The
aim of the present study was to demonstrate the key role of differential
expression levels of RB1, TP53, XIAP, BCL2 AIFM3, BAX, CASP3 and CASP9 genes in
odontogenic cysts. Materials and Methods:
A total number of 15 patients who diagnosed with odontogenic cyst were enrolled
for the present study. For the quantitative gene expression analysis, cyst and
adjacent gingival healthy tissues of patients were collected during surgical assessments.
Quantitative analysis of gene expression levels RB1, TP53, XIAP, BCL2 AIFM3,
BAX, CASP3 and CASP9 were achieved real-time PCR method. For the optimization
of gene expression levels GAPDH reference gene was used. Results:
Expression of both RB1 and TP53 genes were markedly diminished in odontogenic
cysts tissues as compared to healthy tissues (p<0.05). Likewise, levels of
CASP3 and CASP9 genes were found to be significantly reduced in odontogenic
cysts tissues compared to healthy tissues (p<0.05). In contrast, expression levels
of XIAP was significantly elevated (p<0.05). Although BCL2, AIFM3, and BAX
genes were also differentially expressed in odontogenic cysts tissues, these variations
were statistically insignificant (p>0.05).
Conclusions:
The findings of the present study indicates that RB1, TP53, XIAP, CASP3 and
CASP9 genes might have chief roles in formation odontogenic cysts and
responsible for the increased cell proliferation in these tissues. |
| first_indexed | 2024-03-12T05:10:33Z |
| format | Article |
| id | doaj.art-441ad731d0704a19a6b9bcf3c354885e |
| institution | Directory Open Access Journal |
| issn | 1302-5805 2146-2852 |
| language | English |
| last_indexed | 2024-03-12T05:10:33Z |
| publishDate | 2019-09-01 |
| publisher | Cumhuriyet University |
| record_format | Article |
| series | Cumhuriyet Dental Journal |
| spelling | doaj.art-441ad731d0704a19a6b9bcf3c354885e2023-09-03T08:41:30ZengCumhuriyet UniversityCumhuriyet Dental Journal1302-58052146-28522019-09-0122326827510.7126/cumudj.5438752057Deregulation of Cancer-Associated Genes in Odontogenic Cystsİbrahim Bozgeyik0Önder Yumrutaş1Bilal Ege2ADIYAMAN ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ BİYOLOJİ ANABİLİM DALIADIYAMAN ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ BİYOLOJİ ANABİLİM DALIADIYAMAN ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ, KLİNİK BİLİMLER BÖLÜMÜ, AĞIZ, DİŞ VE ÇENE CERRAHİSİ ANABİLİM DALIObjectives: The aim of the present study was to demonstrate the key role of differential expression levels of RB1, TP53, XIAP, BCL2 AIFM3, BAX, CASP3 and CASP9 genes in odontogenic cysts. Materials and Methods: A total number of 15 patients who diagnosed with odontogenic cyst were enrolled for the present study. For the quantitative gene expression analysis, cyst and adjacent gingival healthy tissues of patients were collected during surgical assessments. Quantitative analysis of gene expression levels RB1, TP53, XIAP, BCL2 AIFM3, BAX, CASP3 and CASP9 were achieved real-time PCR method. For the optimization of gene expression levels GAPDH reference gene was used. Results: Expression of both RB1 and TP53 genes were markedly diminished in odontogenic cysts tissues as compared to healthy tissues (p<0.05). Likewise, levels of CASP3 and CASP9 genes were found to be significantly reduced in odontogenic cysts tissues compared to healthy tissues (p<0.05). In contrast, expression levels of XIAP was significantly elevated (p<0.05). Although BCL2, AIFM3, and BAX genes were also differentially expressed in odontogenic cysts tissues, these variations were statistically insignificant (p>0.05). Conclusions: The findings of the present study indicates that RB1, TP53, XIAP, CASP3 and CASP9 genes might have chief roles in formation odontogenic cysts and responsible for the increased cell proliferation in these tissues.https://dergipark.org.tr/tr/download/article-file/803479gene expressionodontojenic cyststumor suppressor gene |
| spellingShingle | İbrahim Bozgeyik Önder Yumrutaş Bilal Ege Deregulation of Cancer-Associated Genes in Odontogenic Cysts Cumhuriyet Dental Journal gene expression odontojenic cysts tumor suppressor gene |
| title | Deregulation of Cancer-Associated Genes in Odontogenic Cysts |
| title_full | Deregulation of Cancer-Associated Genes in Odontogenic Cysts |
| title_fullStr | Deregulation of Cancer-Associated Genes in Odontogenic Cysts |
| title_full_unstemmed | Deregulation of Cancer-Associated Genes in Odontogenic Cysts |
| title_short | Deregulation of Cancer-Associated Genes in Odontogenic Cysts |
| title_sort | deregulation of cancer associated genes in odontogenic cysts |
| topic | gene expression odontojenic cysts tumor suppressor gene |
| url | https://dergipark.org.tr/tr/download/article-file/803479 |
| work_keys_str_mv | AT ibrahimbozgeyik deregulationofcancerassociatedgenesinodontogeniccysts AT onderyumrutas deregulationofcancerassociatedgenesinodontogeniccysts AT bilalege deregulationofcancerassociatedgenesinodontogeniccysts |