<i>n</i>-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8<sup>+</sup> T Cells

The immune-suppressive effects of omega-3 (<i>n</i>-3) polyunsaturated fatty acids (PUFAs) on T cells have been observed via multiple in vitro and in vivo models. However, the precise mechanism that causes these effects is still undefined. In this study, we investigated whether <i>...

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Main Authors: Younghyun Lim, Seyoung Kim, Sehoon Kim, Dong-In Kim, Kyung Won Kang, So-Hee Hong, Sang-Myeong Lee, Hye Ran Koh, Young-Jin Seo
Format: Article
Language:English
Published: MDPI AG 2020-06-01
Series:Viruses
Subjects:
Online Access:https://www.mdpi.com/1999-4915/12/6/639
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author Younghyun Lim
Seyoung Kim
Sehoon Kim
Dong-In Kim
Kyung Won Kang
So-Hee Hong
Sang-Myeong Lee
Hye Ran Koh
Young-Jin Seo
author_facet Younghyun Lim
Seyoung Kim
Sehoon Kim
Dong-In Kim
Kyung Won Kang
So-Hee Hong
Sang-Myeong Lee
Hye Ran Koh
Young-Jin Seo
author_sort Younghyun Lim
collection DOAJ
description The immune-suppressive effects of omega-3 (<i>n</i>-3) polyunsaturated fatty acids (PUFAs) on T cells have been observed via multiple in vitro and in vivo models. However, the precise mechanism that causes these effects is still undefined. In this study, we investigated whether <i>n</i>-3 PUFAs regulated T cell receptor (TCR) and peptide-major histocompatibility complex (pMHC) interactions. The expansion of anti-viral CD8<sup>+</sup> T cells that endogenously synthesize <i>n</i>-3 PUFAs (FAT-1) dramatically decreased upon lymphocytic choriomeningitis virus (LCMV) infection in vivo. This decrease was not caused by the considerable reduction of TCR expression or the impaired chemotactic activity of T cells. Interestingly, a highly inclined and laminated optical sheet (HILO) microscopic analysis revealed that the TCR motility was notably reduced on the surface of the FAT-1 CD8<sup>+</sup> T cells compared to the wild type (WT) CD8<sup>+</sup> T cells. Importantly, the adhesion strength of the FAT-1 CD8<sup>+</sup> T cells to the peptide-MHC was significantly lower than that of the WT CD8<sup>+</sup>T cells. Consistent with this result, treatment with docosahexaenoic acid (<i>DHA</i>), one type of <i>n</i>-3 PUFA, significantly decreased CD8<sup>+</sup> T cell adhesion to the pMHC. Collectively, our results reveal a novel mechanism through which <i>n</i>-3 PUFAs decrease TCR-pMHC interactions by modulating TCR mobility on CD8<sup>+</sup> T cell surfaces.
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spelling doaj.art-441b918717514cd5a77a178e5cd8be482023-11-20T03:36:06ZengMDPI AGViruses1999-49152020-06-0112663910.3390/v12060639<i>n</i>-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8<sup>+</sup> T CellsYounghyun Lim0Seyoung Kim1Sehoon Kim2Dong-In Kim3Kyung Won Kang4So-Hee Hong5Sang-Myeong Lee6Hye Ran Koh7Young-Jin Seo8Department of Life Science, Chung-Ang University, Seoul 06974, KoreaDepartment of Life Science, Chung-Ang University, Seoul 06974, KoreaDepartment of Chemistry, Chung-Ang University, Seoul 06974, KoreaDepartment of Life Science, Chung-Ang University, Seoul 06974, KoreaDivision of Biotechnology, College of Environmental and Bioresources, Jeonbuk National University, Iksan 54596, KoreaDepartment of Biotechnology, the Catholic University of Korea, Bucheon 14662, KoreaDivision of Biotechnology, College of Environmental and Bioresources, Jeonbuk National University, Iksan 54596, KoreaDepartment of Chemistry, Chung-Ang University, Seoul 06974, KoreaDepartment of Life Science, Chung-Ang University, Seoul 06974, KoreaThe immune-suppressive effects of omega-3 (<i>n</i>-3) polyunsaturated fatty acids (PUFAs) on T cells have been observed via multiple in vitro and in vivo models. However, the precise mechanism that causes these effects is still undefined. In this study, we investigated whether <i>n</i>-3 PUFAs regulated T cell receptor (TCR) and peptide-major histocompatibility complex (pMHC) interactions. The expansion of anti-viral CD8<sup>+</sup> T cells that endogenously synthesize <i>n</i>-3 PUFAs (FAT-1) dramatically decreased upon lymphocytic choriomeningitis virus (LCMV) infection in vivo. This decrease was not caused by the considerable reduction of TCR expression or the impaired chemotactic activity of T cells. Interestingly, a highly inclined and laminated optical sheet (HILO) microscopic analysis revealed that the TCR motility was notably reduced on the surface of the FAT-1 CD8<sup>+</sup> T cells compared to the wild type (WT) CD8<sup>+</sup> T cells. Importantly, the adhesion strength of the FAT-1 CD8<sup>+</sup> T cells to the peptide-MHC was significantly lower than that of the WT CD8<sup>+</sup>T cells. Consistent with this result, treatment with docosahexaenoic acid (<i>DHA</i>), one type of <i>n</i>-3 PUFA, significantly decreased CD8<sup>+</sup> T cell adhesion to the pMHC. Collectively, our results reveal a novel mechanism through which <i>n</i>-3 PUFAs decrease TCR-pMHC interactions by modulating TCR mobility on CD8<sup>+</sup> T cell surfaces.https://www.mdpi.com/1999-4915/12/6/639omega-3CD8<sup>+</sup> T cellsLCMVTCR-pMHC interaction
spellingShingle Younghyun Lim
Seyoung Kim
Sehoon Kim
Dong-In Kim
Kyung Won Kang
So-Hee Hong
Sang-Myeong Lee
Hye Ran Koh
Young-Jin Seo
<i>n</i>-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8<sup>+</sup> T Cells
Viruses
omega-3
CD8<sup>+</sup> T cells
LCMV
TCR-pMHC interaction
title <i>n</i>-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8<sup>+</sup> T Cells
title_full <i>n</i>-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8<sup>+</sup> T Cells
title_fullStr <i>n</i>-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8<sup>+</sup> T Cells
title_full_unstemmed <i>n</i>-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8<sup>+</sup> T Cells
title_short <i>n</i>-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8<sup>+</sup> T Cells
title_sort i n i 3 polyunsaturated fatty acids impede the tcr mobility and the tcr pmhc interaction of anti viral cd8 sup sup t cells
topic omega-3
CD8<sup>+</sup> T cells
LCMV
TCR-pMHC interaction
url https://www.mdpi.com/1999-4915/12/6/639
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