<i>n</i>-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8<sup>+</sup> T Cells
The immune-suppressive effects of omega-3 (<i>n</i>-3) polyunsaturated fatty acids (PUFAs) on T cells have been observed via multiple in vitro and in vivo models. However, the precise mechanism that causes these effects is still undefined. In this study, we investigated whether <i>...
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2020-06-01
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author | Younghyun Lim Seyoung Kim Sehoon Kim Dong-In Kim Kyung Won Kang So-Hee Hong Sang-Myeong Lee Hye Ran Koh Young-Jin Seo |
author_facet | Younghyun Lim Seyoung Kim Sehoon Kim Dong-In Kim Kyung Won Kang So-Hee Hong Sang-Myeong Lee Hye Ran Koh Young-Jin Seo |
author_sort | Younghyun Lim |
collection | DOAJ |
description | The immune-suppressive effects of omega-3 (<i>n</i>-3) polyunsaturated fatty acids (PUFAs) on T cells have been observed via multiple in vitro and in vivo models. However, the precise mechanism that causes these effects is still undefined. In this study, we investigated whether <i>n</i>-3 PUFAs regulated T cell receptor (TCR) and peptide-major histocompatibility complex (pMHC) interactions. The expansion of anti-viral CD8<sup>+</sup> T cells that endogenously synthesize <i>n</i>-3 PUFAs (FAT-1) dramatically decreased upon lymphocytic choriomeningitis virus (LCMV) infection in vivo. This decrease was not caused by the considerable reduction of TCR expression or the impaired chemotactic activity of T cells. Interestingly, a highly inclined and laminated optical sheet (HILO) microscopic analysis revealed that the TCR motility was notably reduced on the surface of the FAT-1 CD8<sup>+</sup> T cells compared to the wild type (WT) CD8<sup>+</sup> T cells. Importantly, the adhesion strength of the FAT-1 CD8<sup>+</sup> T cells to the peptide-MHC was significantly lower than that of the WT CD8<sup>+</sup>T cells. Consistent with this result, treatment with docosahexaenoic acid (<i>DHA</i>), one type of <i>n</i>-3 PUFA, significantly decreased CD8<sup>+</sup> T cell adhesion to the pMHC. Collectively, our results reveal a novel mechanism through which <i>n</i>-3 PUFAs decrease TCR-pMHC interactions by modulating TCR mobility on CD8<sup>+</sup> T cell surfaces. |
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id | doaj.art-441b918717514cd5a77a178e5cd8be48 |
institution | Directory Open Access Journal |
issn | 1999-4915 |
language | English |
last_indexed | 2024-03-10T19:12:52Z |
publishDate | 2020-06-01 |
publisher | MDPI AG |
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series | Viruses |
spelling | doaj.art-441b918717514cd5a77a178e5cd8be482023-11-20T03:36:06ZengMDPI AGViruses1999-49152020-06-0112663910.3390/v12060639<i>n</i>-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8<sup>+</sup> T CellsYounghyun Lim0Seyoung Kim1Sehoon Kim2Dong-In Kim3Kyung Won Kang4So-Hee Hong5Sang-Myeong Lee6Hye Ran Koh7Young-Jin Seo8Department of Life Science, Chung-Ang University, Seoul 06974, KoreaDepartment of Life Science, Chung-Ang University, Seoul 06974, KoreaDepartment of Chemistry, Chung-Ang University, Seoul 06974, KoreaDepartment of Life Science, Chung-Ang University, Seoul 06974, KoreaDivision of Biotechnology, College of Environmental and Bioresources, Jeonbuk National University, Iksan 54596, KoreaDepartment of Biotechnology, the Catholic University of Korea, Bucheon 14662, KoreaDivision of Biotechnology, College of Environmental and Bioresources, Jeonbuk National University, Iksan 54596, KoreaDepartment of Chemistry, Chung-Ang University, Seoul 06974, KoreaDepartment of Life Science, Chung-Ang University, Seoul 06974, KoreaThe immune-suppressive effects of omega-3 (<i>n</i>-3) polyunsaturated fatty acids (PUFAs) on T cells have been observed via multiple in vitro and in vivo models. However, the precise mechanism that causes these effects is still undefined. In this study, we investigated whether <i>n</i>-3 PUFAs regulated T cell receptor (TCR) and peptide-major histocompatibility complex (pMHC) interactions. The expansion of anti-viral CD8<sup>+</sup> T cells that endogenously synthesize <i>n</i>-3 PUFAs (FAT-1) dramatically decreased upon lymphocytic choriomeningitis virus (LCMV) infection in vivo. This decrease was not caused by the considerable reduction of TCR expression or the impaired chemotactic activity of T cells. Interestingly, a highly inclined and laminated optical sheet (HILO) microscopic analysis revealed that the TCR motility was notably reduced on the surface of the FAT-1 CD8<sup>+</sup> T cells compared to the wild type (WT) CD8<sup>+</sup> T cells. Importantly, the adhesion strength of the FAT-1 CD8<sup>+</sup> T cells to the peptide-MHC was significantly lower than that of the WT CD8<sup>+</sup>T cells. Consistent with this result, treatment with docosahexaenoic acid (<i>DHA</i>), one type of <i>n</i>-3 PUFA, significantly decreased CD8<sup>+</sup> T cell adhesion to the pMHC. Collectively, our results reveal a novel mechanism through which <i>n</i>-3 PUFAs decrease TCR-pMHC interactions by modulating TCR mobility on CD8<sup>+</sup> T cell surfaces.https://www.mdpi.com/1999-4915/12/6/639omega-3CD8<sup>+</sup> T cellsLCMVTCR-pMHC interaction |
spellingShingle | Younghyun Lim Seyoung Kim Sehoon Kim Dong-In Kim Kyung Won Kang So-Hee Hong Sang-Myeong Lee Hye Ran Koh Young-Jin Seo <i>n</i>-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8<sup>+</sup> T Cells Viruses omega-3 CD8<sup>+</sup> T cells LCMV TCR-pMHC interaction |
title | <i>n</i>-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8<sup>+</sup> T Cells |
title_full | <i>n</i>-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8<sup>+</sup> T Cells |
title_fullStr | <i>n</i>-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8<sup>+</sup> T Cells |
title_full_unstemmed | <i>n</i>-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8<sup>+</sup> T Cells |
title_short | <i>n</i>-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8<sup>+</sup> T Cells |
title_sort | i n i 3 polyunsaturated fatty acids impede the tcr mobility and the tcr pmhc interaction of anti viral cd8 sup sup t cells |
topic | omega-3 CD8<sup>+</sup> T cells LCMV TCR-pMHC interaction |
url | https://www.mdpi.com/1999-4915/12/6/639 |
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