Novel Proteoliposome-Based Vaccine against <i>E. coli</i>: A Potential New Tool for the Control of Bovine Mastitis
<i>Escherichia coli</i> is an important causative agent of clinical mastitis in cattle. Current available vaccines have shown limited protection. We evaluated the efficacy of a novel vaccine based on bacterial proteoliposomes derived from an <i>E. coli</i> field strain. Femal...
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MDPI AG
2022-09-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/19/2533 |
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| author | John Quiroga Sonia Vidal Daniela Siel Mario Caruffo Andrea Valdés Gonzalo Cabrera Lissette Lapierre Leonardo Sáenz |
| author_facet | John Quiroga Sonia Vidal Daniela Siel Mario Caruffo Andrea Valdés Gonzalo Cabrera Lissette Lapierre Leonardo Sáenz |
| author_sort | John Quiroga |
| collection | DOAJ |
| description | <i>Escherichia coli</i> is an important causative agent of clinical mastitis in cattle. Current available vaccines have shown limited protection. We evaluated the efficacy of a novel vaccine based on bacterial proteoliposomes derived from an <i>E. coli</i> field strain. Female BALB/c mice were immunized subcutaneously with two doses of the vaccine, 3 weeks apart. Between days 5 and 8 after the first inoculation, the females were mated. At 5–8 days postpartum, the mice were intramammary challenged with the same <i>E. coli</i> strain. Two days after bacterial infection, mice were euthanized, and the mammary glands were examined and removed to evaluate the efficacy and safety of the vaccine as well as the immune response generated by the new formulation. The vaccinated mice showed mild clinical symptoms and a lower mammary bacterial load as compared to non-vaccinated animals. The vaccination induced an increase in levels of IgG, IgG1 and IgG2a against <i>E. coli</i> in blood and mammary glands that showed less inflammatory infiltration and tissue damage, as compared to the control group. In summary, the vaccine based on bacterial proteoliposomes is safe, immunogenic, and effective against <i>E. coli</i>, constituting a new potential tool for mastitis control. |
| first_indexed | 2024-03-09T22:09:16Z |
| format | Article |
| id | doaj.art-4420f64949e24c22910d13ffbadf50d2 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T22:09:16Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-4420f64949e24c22910d13ffbadf50d22023-11-23T19:35:17ZengMDPI AGAnimals2076-26152022-09-011219253310.3390/ani12192533Novel Proteoliposome-Based Vaccine against <i>E. coli</i>: A Potential New Tool for the Control of Bovine MastitisJohn Quiroga0Sonia Vidal1Daniela Siel2Mario Caruffo3Andrea Valdés4Gonzalo Cabrera5Lissette Lapierre6Leonardo Sáenz7Faculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, ChilePrograma de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago 8380000, ChileFaculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, ChileFaculty of Veterinary Sciences, Universidad de Chile, Santiago 8820808, Chile<i>Escherichia coli</i> is an important causative agent of clinical mastitis in cattle. Current available vaccines have shown limited protection. We evaluated the efficacy of a novel vaccine based on bacterial proteoliposomes derived from an <i>E. coli</i> field strain. Female BALB/c mice were immunized subcutaneously with two doses of the vaccine, 3 weeks apart. Between days 5 and 8 after the first inoculation, the females were mated. At 5–8 days postpartum, the mice were intramammary challenged with the same <i>E. coli</i> strain. Two days after bacterial infection, mice were euthanized, and the mammary glands were examined and removed to evaluate the efficacy and safety of the vaccine as well as the immune response generated by the new formulation. The vaccinated mice showed mild clinical symptoms and a lower mammary bacterial load as compared to non-vaccinated animals. The vaccination induced an increase in levels of IgG, IgG1 and IgG2a against <i>E. coli</i> in blood and mammary glands that showed less inflammatory infiltration and tissue damage, as compared to the control group. In summary, the vaccine based on bacterial proteoliposomes is safe, immunogenic, and effective against <i>E. coli</i>, constituting a new potential tool for mastitis control.https://www.mdpi.com/2076-2615/12/19/2533proteoliposomesmastitismurine model<i>Escherichia coli</i> |
| spellingShingle | John Quiroga Sonia Vidal Daniela Siel Mario Caruffo Andrea Valdés Gonzalo Cabrera Lissette Lapierre Leonardo Sáenz Novel Proteoliposome-Based Vaccine against <i>E. coli</i>: A Potential New Tool for the Control of Bovine Mastitis Animals proteoliposomes mastitis murine model <i>Escherichia coli</i> |
| title | Novel Proteoliposome-Based Vaccine against <i>E. coli</i>: A Potential New Tool for the Control of Bovine Mastitis |
| title_full | Novel Proteoliposome-Based Vaccine against <i>E. coli</i>: A Potential New Tool for the Control of Bovine Mastitis |
| title_fullStr | Novel Proteoliposome-Based Vaccine against <i>E. coli</i>: A Potential New Tool for the Control of Bovine Mastitis |
| title_full_unstemmed | Novel Proteoliposome-Based Vaccine against <i>E. coli</i>: A Potential New Tool for the Control of Bovine Mastitis |
| title_short | Novel Proteoliposome-Based Vaccine against <i>E. coli</i>: A Potential New Tool for the Control of Bovine Mastitis |
| title_sort | novel proteoliposome based vaccine against i e coli i a potential new tool for the control of bovine mastitis |
| topic | proteoliposomes mastitis murine model <i>Escherichia coli</i> |
| url | https://www.mdpi.com/2076-2615/12/19/2533 |
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