Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-Teratozoospermia
In recent years, microfluidic chip-based sperm sorting has emerged as an alternative tool to centrifugation-based conventional techniques for in vitro fertilization. This prospective study aims to compare the effects of density gradient centrifugation and microfluidic chip sperm preparation methods...
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MDPI AG
2021-09-01
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| author | Cagla Guler Sureyya Melil Umit Ozekici Yaprak Donmez Cakil Belgin Selam Mehmet Cincik |
| author_facet | Cagla Guler Sureyya Melil Umit Ozekici Yaprak Donmez Cakil Belgin Selam Mehmet Cincik |
| author_sort | Cagla Guler |
| collection | DOAJ |
| description | In recent years, microfluidic chip-based sperm sorting has emerged as an alternative tool to centrifugation-based conventional techniques for in vitro fertilization. This prospective study aims to compare the effects of density gradient centrifugation and microfluidic chip sperm preparation methods on embryo development in patient populations with astheno-teratozoospermia. In the study, the semen samples of the patients were divided into two groups for preparation with either the microfluidic or density gradient methods. Selected spermatozoa were then used to fertilize mature sibling oocytes and the semen parameters and embryo development on days 3 and 5 were assessed. While the density gradient group was associated with a higher sperm concentration, motility (progressive and total) was significantly higher in the microfluidic chip group. No significant differences were observed in the fertilization rates or grade 1 (G1) and grade 2 (G2) proportions of the third-day embryos. Furthermore, while the proportions of the poor, fair and good blastocysts on day 5 did not differ significantly, excellent blastocysts (indicating high-quality embryos) were observed in a significantly higher proportion of the microfluidic chip group. When compared to the classical density gradient method, the microfluidic chip sperm preparation yielded sperm with higher motility and higher quality blastocysts at day 5; in patients with astheno-teratozoospermia. |
| first_indexed | 2024-03-10T07:29:50Z |
| format | Article |
| id | doaj.art-44220bdc921d4536926669f4bdb63ec5 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T07:29:50Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-44220bdc921d4536926669f4bdb63ec52023-11-22T13:56:06ZengMDPI AGLife2075-17292021-09-0111993310.3390/life11090933Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-TeratozoospermiaCagla Guler0Sureyya Melil1Umit Ozekici2Yaprak Donmez Cakil3Belgin Selam4Mehmet Cincik5Institute of Health Sciences, Maltepe University, 34857 Istanbul, TurkeyIVF Clinic, Maltepe University Hospital, 34844 Istanbul, TurkeyIVF Clinic, Maltepe University Hospital, 34844 Istanbul, TurkeyDepartment of Histology and Embryology, Faculty of Medicine, Maltepe University, 34857 Istanbul, TurkeyDepartment of Obstetrics and Gynecology, School of Medicine, Acibadem Mehmet Ali Aydinlar University, Unit of ART, Acibadem Altunizade Hospital, 34662 Istanbul, TurkeyInstitute of Health Sciences, Maltepe University, 34857 Istanbul, TurkeyIn recent years, microfluidic chip-based sperm sorting has emerged as an alternative tool to centrifugation-based conventional techniques for in vitro fertilization. This prospective study aims to compare the effects of density gradient centrifugation and microfluidic chip sperm preparation methods on embryo development in patient populations with astheno-teratozoospermia. In the study, the semen samples of the patients were divided into two groups for preparation with either the microfluidic or density gradient methods. Selected spermatozoa were then used to fertilize mature sibling oocytes and the semen parameters and embryo development on days 3 and 5 were assessed. While the density gradient group was associated with a higher sperm concentration, motility (progressive and total) was significantly higher in the microfluidic chip group. No significant differences were observed in the fertilization rates or grade 1 (G1) and grade 2 (G2) proportions of the third-day embryos. Furthermore, while the proportions of the poor, fair and good blastocysts on day 5 did not differ significantly, excellent blastocysts (indicating high-quality embryos) were observed in a significantly higher proportion of the microfluidic chip group. When compared to the classical density gradient method, the microfluidic chip sperm preparation yielded sperm with higher motility and higher quality blastocysts at day 5; in patients with astheno-teratozoospermia.https://www.mdpi.com/2075-1729/11/9/933male infertilityastheno-teratozoospermiamicrochipdensity gradient centrifugationembryoblastocyst |
| spellingShingle | Cagla Guler Sureyya Melil Umit Ozekici Yaprak Donmez Cakil Belgin Selam Mehmet Cincik Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-Teratozoospermia Life male infertility astheno-teratozoospermia microchip density gradient centrifugation embryo blastocyst |
| title | Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-Teratozoospermia |
| title_full | Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-Teratozoospermia |
| title_fullStr | Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-Teratozoospermia |
| title_full_unstemmed | Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-Teratozoospermia |
| title_short | Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-Teratozoospermia |
| title_sort | sperm selection and embryo development a comparison of the density gradient centrifugation and microfluidic chip sperm preparation methods in patients with astheno teratozoospermia |
| topic | male infertility astheno-teratozoospermia microchip density gradient centrifugation embryo blastocyst |
| url | https://www.mdpi.com/2075-1729/11/9/933 |
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