Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model
Temporary scaffolds that mimic the extracellular matrix’s structure and provide a stable substratum for the natural growth of cells are an innovative trend in the field of tissue engineering. The aim of this study is to obtain and design porous 2D fibroin-based cell matrices by femtosecond laser-ind...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/13/2584 |
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| author | Liliya Angelova Albena Daskalova Emil Filipov Xavier Monforte Vila Janine Tomasch Georgi Avdeev Andreas H. Teuschl-Woller Ivan Buchvarov |
| author_facet | Liliya Angelova Albena Daskalova Emil Filipov Xavier Monforte Vila Janine Tomasch Georgi Avdeev Andreas H. Teuschl-Woller Ivan Buchvarov |
| author_sort | Liliya Angelova |
| collection | DOAJ |
| description | Temporary scaffolds that mimic the extracellular matrix’s structure and provide a stable substratum for the natural growth of cells are an innovative trend in the field of tissue engineering. The aim of this study is to obtain and design porous 2D fibroin-based cell matrices by femtosecond laser-induced microstructuring for future applications in muscle tissue engineering. Ultra-fast laser treatment is a non-contact method, which generates controlled porosity—the creation of micro/nanostructures on the surface of the biopolymer that can strongly affect cell behavior, while the control over its surface characteristics has the potential of directing the growth of future muscle tissue in the desired direction. The laser structured 2D thin film matrices from silk were characterized by means of SEM, EDX, AFM, FTIR, Micro-Raman, XRD, and 3D-roughness analyses. A WCA evaluation and initial experiments with murine C2C12 myoblasts cells were also performed. The results show that by varying the laser parameters, a different structuring degree can be achieved through the initial lifting and ejection of the material around the area of laser interaction to generate porous channels with varying widths and depths. The proper optimization of the applied laser parameters can significantly improve the bioactive properties of the investigated 2D model of a muscle cell matrix. |
| first_indexed | 2024-03-09T12:38:43Z |
| format | Article |
| id | doaj.art-44242f33636641fc99ffced55922a4fa |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T12:38:43Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-44242f33636641fc99ffced55922a4fa2023-11-30T22:20:59ZengMDPI AGPolymers2073-43602022-06-011413258410.3390/polym14132584Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix ModelLiliya Angelova0Albena Daskalova1Emil Filipov2Xavier Monforte Vila3Janine Tomasch4Georgi Avdeev5Andreas H. Teuschl-Woller6Ivan Buchvarov7Institute of Electronics, Bulgarian Academy of Sciences, 72 Tzarigradsko Shousse Blvd., 1784 Sofia, BulgariaInstitute of Electronics, Bulgarian Academy of Sciences, 72 Tzarigradsko Shousse Blvd., 1784 Sofia, BulgariaInstitute of Electronics, Bulgarian Academy of Sciences, 72 Tzarigradsko Shousse Blvd., 1784 Sofia, BulgariaDepartment Life Science Engineering, University of Applied Sciences Technikum Wien, Höchstädtplatz 6, 1200 Vienna, AustriaDepartment Life Science Engineering, University of Applied Sciences Technikum Wien, Höchstädtplatz 6, 1200 Vienna, AustriaInstitute of Physical Chemistry, Bulgarian Academy of Sciences, Akad. G. Bonchev Str., 1113 Sofia, BulgariaDepartment Life Science Engineering, University of Applied Sciences Technikum Wien, Höchstädtplatz 6, 1200 Vienna, AustriaFaculty of Physics, St. Kliment Ohridski University of Sofia, 5 James Bourchier Blvd., 1164 Sofia, BulgariaTemporary scaffolds that mimic the extracellular matrix’s structure and provide a stable substratum for the natural growth of cells are an innovative trend in the field of tissue engineering. The aim of this study is to obtain and design porous 2D fibroin-based cell matrices by femtosecond laser-induced microstructuring for future applications in muscle tissue engineering. Ultra-fast laser treatment is a non-contact method, which generates controlled porosity—the creation of micro/nanostructures on the surface of the biopolymer that can strongly affect cell behavior, while the control over its surface characteristics has the potential of directing the growth of future muscle tissue in the desired direction. The laser structured 2D thin film matrices from silk were characterized by means of SEM, EDX, AFM, FTIR, Micro-Raman, XRD, and 3D-roughness analyses. A WCA evaluation and initial experiments with murine C2C12 myoblasts cells were also performed. The results show that by varying the laser parameters, a different structuring degree can be achieved through the initial lifting and ejection of the material around the area of laser interaction to generate porous channels with varying widths and depths. The proper optimization of the applied laser parameters can significantly improve the bioactive properties of the investigated 2D model of a muscle cell matrix.https://www.mdpi.com/2073-4360/14/13/2584silk fibroinbiopolymersfemtosecond laser processingmuscle tissue engineeringmuscle cell matrix 2D model |
| spellingShingle | Liliya Angelova Albena Daskalova Emil Filipov Xavier Monforte Vila Janine Tomasch Georgi Avdeev Andreas H. Teuschl-Woller Ivan Buchvarov Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model Polymers silk fibroin biopolymers femtosecond laser processing muscle tissue engineering muscle cell matrix 2D model |
| title | Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model |
| title_full | Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model |
| title_fullStr | Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model |
| title_full_unstemmed | Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model |
| title_short | Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model |
| title_sort | optimizing the surface structural and morphological properties of silk thin films via ultra short laser texturing for creation of muscle cell matrix model |
| topic | silk fibroin biopolymers femtosecond laser processing muscle tissue engineering muscle cell matrix 2D model |
| url | https://www.mdpi.com/2073-4360/14/13/2584 |
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