Diabetes-Independent Retinal Phenotypes in an Aldose Reductase Transgenic Mouse Model
Aldose reductase (AR), the first and rate-limiting enzyme of the polyol pathway, has been implicated in the onset and development of the ocular complications of diabetes, including cataracts and retinopathy. Despite decades of research conducted to address possible mechanisms, questions still persis...
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MDPI AG
2021-07-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/7/450 |
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| author | Jonathan Mark Petrash Biehuoy Shieh David A. Ammar Michelle G. Pedler David J. Orlicky |
| author_facet | Jonathan Mark Petrash Biehuoy Shieh David A. Ammar Michelle G. Pedler David J. Orlicky |
| author_sort | Jonathan Mark Petrash |
| collection | DOAJ |
| description | Aldose reductase (AR), the first and rate-limiting enzyme of the polyol pathway, has been implicated in the onset and development of the ocular complications of diabetes, including cataracts and retinopathy. Despite decades of research conducted to address possible mechanisms, questions still persist in understanding if or how AR contributes to imbalances leading to diabetic eye disease. To address these questions, we created a strain of transgenic mice engineered for the overexpression of human AR (AR-Tg). In the course of monitoring these animals for age-related retinal phenotypes, we observed signs of Müller cell gliosis characterized by strong immunostaining for glial fibrillary acidic protein. In addition, we observed increased staining for Iba1, consistent with an increase in the number of retinal microglia, a marker of retinal inflammation. Compared to age-matched nontransgenic controls, AR-Tg mice showed an age-dependent loss of Brn3a-positive retinal ganglion cells and an associated decrease in PERG amplitude. Both RGC-related phenotypes were rescued in animals treated with Sorbinil in drinking water. These results support the hypothesis that increased levels of AR may be a risk factor for structural and functional changes known to accompany retinopathy in humans. |
| first_indexed | 2024-03-10T09:32:16Z |
| format | Article |
| id | doaj.art-442620712d104a0c9d6576385511f12e |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T09:32:16Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-442620712d104a0c9d6576385511f12e2023-11-22T04:22:56ZengMDPI AGMetabolites2218-19892021-07-0111745010.3390/metabo11070450Diabetes-Independent Retinal Phenotypes in an Aldose Reductase Transgenic Mouse ModelJonathan Mark Petrash0Biehuoy Shieh1David A. Ammar2Michelle G. Pedler3David J. Orlicky4Department of Ophthalmology, University of Colorado School of Medicine, 12800 E. 19th Ave., Aurora, CO 80045, USADepartment of Ophthalmology, University of Colorado School of Medicine, 12800 E. 19th Ave., Aurora, CO 80045, USALions Eye Institute for Transplant and Research, 1410 N 21st St, Tampa, FL 33605, USADepartment of Ophthalmology, University of Colorado School of Medicine, 12800 E. 19th Ave., Aurora, CO 80045, USADepartment of Pathology, University of Colorado School of Medicine, 12800 E. 19th Ave., Aurora, CO 80045, USAAldose reductase (AR), the first and rate-limiting enzyme of the polyol pathway, has been implicated in the onset and development of the ocular complications of diabetes, including cataracts and retinopathy. Despite decades of research conducted to address possible mechanisms, questions still persist in understanding if or how AR contributes to imbalances leading to diabetic eye disease. To address these questions, we created a strain of transgenic mice engineered for the overexpression of human AR (AR-Tg). In the course of monitoring these animals for age-related retinal phenotypes, we observed signs of Müller cell gliosis characterized by strong immunostaining for glial fibrillary acidic protein. In addition, we observed increased staining for Iba1, consistent with an increase in the number of retinal microglia, a marker of retinal inflammation. Compared to age-matched nontransgenic controls, AR-Tg mice showed an age-dependent loss of Brn3a-positive retinal ganglion cells and an associated decrease in PERG amplitude. Both RGC-related phenotypes were rescued in animals treated with Sorbinil in drinking water. These results support the hypothesis that increased levels of AR may be a risk factor for structural and functional changes known to accompany retinopathy in humans.https://www.mdpi.com/2218-1989/11/7/450aldose reductaseMüller gliaretinal ganglion cellSorbinilpattern electroretinogramretinal microglia |
| spellingShingle | Jonathan Mark Petrash Biehuoy Shieh David A. Ammar Michelle G. Pedler David J. Orlicky Diabetes-Independent Retinal Phenotypes in an Aldose Reductase Transgenic Mouse Model Metabolites aldose reductase Müller glia retinal ganglion cell Sorbinil pattern electroretinogram retinal microglia |
| title | Diabetes-Independent Retinal Phenotypes in an Aldose Reductase Transgenic Mouse Model |
| title_full | Diabetes-Independent Retinal Phenotypes in an Aldose Reductase Transgenic Mouse Model |
| title_fullStr | Diabetes-Independent Retinal Phenotypes in an Aldose Reductase Transgenic Mouse Model |
| title_full_unstemmed | Diabetes-Independent Retinal Phenotypes in an Aldose Reductase Transgenic Mouse Model |
| title_short | Diabetes-Independent Retinal Phenotypes in an Aldose Reductase Transgenic Mouse Model |
| title_sort | diabetes independent retinal phenotypes in an aldose reductase transgenic mouse model |
| topic | aldose reductase Müller glia retinal ganglion cell Sorbinil pattern electroretinogram retinal microglia |
| url | https://www.mdpi.com/2218-1989/11/7/450 |
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