In Vitro Assessment of Antistaphylococci, Antitumor, Immunological and Structural Characterization of Acidic Bioactive Exopolysaccharides from Marine <i>Bacillus</i> <i>cereus</i> Isolated from Saudi Arabia
A strain of <i>Bacillus cereus</i> was isolated from the Saudi Red Sea coast and identified based on culture features, biochemical characteristics, and phylogenetic analysis of 16S rRNA sequences. EPSR3 was a major fraction of exopolysaccharides (EP<sub>S</sub>) containing no...
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MDPI AG
2022-02-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/2/132 |
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| author | Samy Selim Mohammed S. Almuhayawi Mohanned Talal Alharbi Mohammed K. Nagshabandi Awadh Alanazi Mona Warrad Nashwa Hagagy Ahmed Ghareeb Abdallah S. Ali |
| author_facet | Samy Selim Mohammed S. Almuhayawi Mohanned Talal Alharbi Mohammed K. Nagshabandi Awadh Alanazi Mona Warrad Nashwa Hagagy Ahmed Ghareeb Abdallah S. Ali |
| author_sort | Samy Selim |
| collection | DOAJ |
| description | A strain of <i>Bacillus cereus</i> was isolated from the Saudi Red Sea coast and identified based on culture features, biochemical characteristics, and phylogenetic analysis of 16S rRNA sequences. EPSR3 was a major fraction of exopolysaccharides (EP<sub>S</sub>) containing no sulfate and had uronic acid (28.7%). The monosaccharide composition of these fractions is composed of glucose, galacturonic acid, and arabinose with a molar ratio of 2.0: 0.8: 1.0, respectively. EPSR3 was subjected to antioxidant, antitumor, and anti-inflammatory activities. The results revealed that the whole antioxidant activity was 90.4 ± 1.6% at 1500 µg/mL after 120 min. So, the IC<sub>50</sub> value against DPPH radical found about 500 µg/mL after 60 min. While using H<sub>2</sub>O<sub>2</sub>, the scavenging activity was 75.1 ± 1.9% at 1500 µg/mL after 60 min. The IC<sub>50</sub> value against H<sub>2</sub>O<sub>2</sub> radical found about 1500 µg/mL after 15 min. EPSR3 anticytotoxic effect on the proliferation of (Bladder carcinoma cell line) (T-24), (human breast carcinoma cell line) (MCF-7), and (human prostate carcinoma cell line) (PC-3) cells. The calculated IC50 for cell line T-24 was 121 ± 4.1 µg/mL, while the IC<sub>50</sub> for cell line MCF-7 was 55.7 ± 2.3 µg/mL, and PC-3 was 61.4 ± 2.6 µg/mL. Anti-inflammatory activity was determined for EPSR3 using different methods as Lipoxygenase (LOX) inhibitory assay gave IC<sub>50</sub> 12.9 ± 1.3 µg/mL. While cyclooxygenase (COX-2) inhibitory test showed 29.6 ± 0.89 µg /mL. EPSR3 showed potent inhibitory activity against methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and coagulase-negative staphylococci. The exposure times of EPSR3 for the complete inhibition of cell viability of methicillin resistant <i>S. aureus</i> was found to be 5% at 60 min. Membrane stabilization inhibitory gave 35.4 ± 0.67 µg/mL. EPSR3 has antitumor activity with a reasonable margin of safety. The antitumor activity of EPSR3 may be attributed to its content from uronic acids with potential for cellular antioxidant and anticancer functional properties. |
| first_indexed | 2024-03-09T21:27:23Z |
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| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T21:27:23Z |
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| spelling | doaj.art-442625abc4a64285b93f3b359607782d2023-11-23T21:04:54ZengMDPI AGMetabolites2218-19892022-02-0112213210.3390/metabo12020132In Vitro Assessment of Antistaphylococci, Antitumor, Immunological and Structural Characterization of Acidic Bioactive Exopolysaccharides from Marine <i>Bacillus</i> <i>cereus</i> Isolated from Saudi ArabiaSamy Selim0Mohammed S. Almuhayawi1Mohanned Talal Alharbi2Mohammed K. Nagshabandi3Awadh Alanazi4Mona Warrad5Nashwa Hagagy6Ahmed Ghareeb7Abdallah S. Ali8Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 72341, Saudi ArabiaDepartment of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Medical Microbiology and Parasitology, Faculty of Medicine, University of Jeddah, Jeddah 23218, Saudi ArabiaDepartment of Medical Microbiology and Parasitology, Faculty of Medicine, University of Jeddah, Jeddah 23218, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 72341, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences at Al-Quriat, Jouf University, Al-Quriat 77454, Saudi ArabiaDepartment of Biology, College of Science and Arts at Khulis, University of Jeddah, Jeddah 21959, Saudi ArabiaBotany and Microbiology Department, Faculty of Science, Cairo University, Giza 12613, EgyptDepartment of Microbiology, Faculty of Agriculture, Cairo University, Giza 12613, EgyptA strain of <i>Bacillus cereus</i> was isolated from the Saudi Red Sea coast and identified based on culture features, biochemical characteristics, and phylogenetic analysis of 16S rRNA sequences. EPSR3 was a major fraction of exopolysaccharides (EP<sub>S</sub>) containing no sulfate and had uronic acid (28.7%). The monosaccharide composition of these fractions is composed of glucose, galacturonic acid, and arabinose with a molar ratio of 2.0: 0.8: 1.0, respectively. EPSR3 was subjected to antioxidant, antitumor, and anti-inflammatory activities. The results revealed that the whole antioxidant activity was 90.4 ± 1.6% at 1500 µg/mL after 120 min. So, the IC<sub>50</sub> value against DPPH radical found about 500 µg/mL after 60 min. While using H<sub>2</sub>O<sub>2</sub>, the scavenging activity was 75.1 ± 1.9% at 1500 µg/mL after 60 min. The IC<sub>50</sub> value against H<sub>2</sub>O<sub>2</sub> radical found about 1500 µg/mL after 15 min. EPSR3 anticytotoxic effect on the proliferation of (Bladder carcinoma cell line) (T-24), (human breast carcinoma cell line) (MCF-7), and (human prostate carcinoma cell line) (PC-3) cells. The calculated IC50 for cell line T-24 was 121 ± 4.1 µg/mL, while the IC<sub>50</sub> for cell line MCF-7 was 55.7 ± 2.3 µg/mL, and PC-3 was 61.4 ± 2.6 µg/mL. Anti-inflammatory activity was determined for EPSR3 using different methods as Lipoxygenase (LOX) inhibitory assay gave IC<sub>50</sub> 12.9 ± 1.3 µg/mL. While cyclooxygenase (COX-2) inhibitory test showed 29.6 ± 0.89 µg /mL. EPSR3 showed potent inhibitory activity against methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and coagulase-negative staphylococci. The exposure times of EPSR3 for the complete inhibition of cell viability of methicillin resistant <i>S. aureus</i> was found to be 5% at 60 min. Membrane stabilization inhibitory gave 35.4 ± 0.67 µg/mL. EPSR3 has antitumor activity with a reasonable margin of safety. The antitumor activity of EPSR3 may be attributed to its content from uronic acids with potential for cellular antioxidant and anticancer functional properties.https://www.mdpi.com/2218-1989/12/2/132AntistaphylococciMRSAantioxidantanti-inflammatoryantitumorExopolysaccharide |
| spellingShingle | Samy Selim Mohammed S. Almuhayawi Mohanned Talal Alharbi Mohammed K. Nagshabandi Awadh Alanazi Mona Warrad Nashwa Hagagy Ahmed Ghareeb Abdallah S. Ali In Vitro Assessment of Antistaphylococci, Antitumor, Immunological and Structural Characterization of Acidic Bioactive Exopolysaccharides from Marine <i>Bacillus</i> <i>cereus</i> Isolated from Saudi Arabia Metabolites Antistaphylococci MRSA antioxidant anti-inflammatory antitumor Exopolysaccharide |
| title | In Vitro Assessment of Antistaphylococci, Antitumor, Immunological and Structural Characterization of Acidic Bioactive Exopolysaccharides from Marine <i>Bacillus</i> <i>cereus</i> Isolated from Saudi Arabia |
| title_full | In Vitro Assessment of Antistaphylococci, Antitumor, Immunological and Structural Characterization of Acidic Bioactive Exopolysaccharides from Marine <i>Bacillus</i> <i>cereus</i> Isolated from Saudi Arabia |
| title_fullStr | In Vitro Assessment of Antistaphylococci, Antitumor, Immunological and Structural Characterization of Acidic Bioactive Exopolysaccharides from Marine <i>Bacillus</i> <i>cereus</i> Isolated from Saudi Arabia |
| title_full_unstemmed | In Vitro Assessment of Antistaphylococci, Antitumor, Immunological and Structural Characterization of Acidic Bioactive Exopolysaccharides from Marine <i>Bacillus</i> <i>cereus</i> Isolated from Saudi Arabia |
| title_short | In Vitro Assessment of Antistaphylococci, Antitumor, Immunological and Structural Characterization of Acidic Bioactive Exopolysaccharides from Marine <i>Bacillus</i> <i>cereus</i> Isolated from Saudi Arabia |
| title_sort | in vitro assessment of antistaphylococci antitumor immunological and structural characterization of acidic bioactive exopolysaccharides from marine i bacillus i i cereus i isolated from saudi arabia |
| topic | Antistaphylococci MRSA antioxidant anti-inflammatory antitumor Exopolysaccharide |
| url | https://www.mdpi.com/2218-1989/12/2/132 |
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