A Cranberry Concentrate Decreases Adhesion and Invasion of <i>Escherichia coli</i> (AIEC) LF82 In Vitro
While many beneficial host–microbiota interactions have been described, imbalanced microbiota in the gut is speculated to contribute to the progression and recurrence of chronic inflammatory diseases such as Crohn’s disease (CD). This in vitro study evaluated the impact of a cranberry concentrate Ty...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/9/1217 |
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| author | Derek Zhang Lynn Verstrepen Jelle De Medts Cindy Duysburgh Pieter Van den Abbeele Massimo Marzorati Christina Khoo |
| author_facet | Derek Zhang Lynn Verstrepen Jelle De Medts Cindy Duysburgh Pieter Van den Abbeele Massimo Marzorati Christina Khoo |
| author_sort | Derek Zhang |
| collection | DOAJ |
| description | While many beneficial host–microbiota interactions have been described, imbalanced microbiota in the gut is speculated to contribute to the progression and recurrence of chronic inflammatory diseases such as Crohn’s disease (CD). This in vitro study evaluated the impact of a cranberry concentrate Type M (CTM) on adherent-invasive <i>Escherichia coli</i> (AIEC) LF82, a pathobiont associated with CD. Different stages of pathogenic infection were investigated: (i) colonization of the mucus layer, and (ii) adhesion to and (iii) invasion of the epithelial cells. Following 48 h of fecal batch incubation, 0.5 and 1 mM of CTM significantly altered AIEC LF82 levels in a simulated mucus layer, resulting in a decrease of 50.5% in the untreated blank, down to 43.0% and 11.4%, respectively. At 1 mM of CTM, the significant decrease in the levels of AIEC LF82 coincided with a stimulation of the metabolic activity of the background microbiota. The increased levels of health-associated acetate (+7.9 mM) and propionate levels (+3.5 mM) suggested selective utilization of CTM by host microorganisms. Furthermore, 1 mM of both fermented and unfermented CTM decreased the adhesion and invasion of human-derived epithelial Caco-2 cells by AIEC LF82. Altogether, this exploratory in vitro study demonstrates the prebiotic potential of CTM and supports its antipathogenic effects through direct and/or indirect modulation of the gut microbiome. |
| first_indexed | 2024-03-10T07:19:10Z |
| format | Article |
| id | doaj.art-4426d1d399c243218c829f573b9761ef |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T07:19:10Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-4426d1d399c243218c829f573b9761ef2023-11-22T14:43:30ZengMDPI AGPathogens2076-08172021-09-01109121710.3390/pathogens10091217A Cranberry Concentrate Decreases Adhesion and Invasion of <i>Escherichia coli</i> (AIEC) LF82 In VitroDerek Zhang0Lynn Verstrepen1Jelle De Medts2Cindy Duysburgh3Pieter Van den Abbeele4Massimo Marzorati5Christina Khoo6Ocean Spray Cranberries, Inc., Lakeville-Middleboro, MA 02349, USAProDigest BV, 9052 Ghent, BelgiumProDigest BV, 9052 Ghent, BelgiumProDigest BV, 9052 Ghent, BelgiumCryptobiotix SA, 9052 Ghent, BelgiumProDigest BV, 9052 Ghent, BelgiumOcean Spray Cranberries, Inc., Lakeville-Middleboro, MA 02349, USAWhile many beneficial host–microbiota interactions have been described, imbalanced microbiota in the gut is speculated to contribute to the progression and recurrence of chronic inflammatory diseases such as Crohn’s disease (CD). This in vitro study evaluated the impact of a cranberry concentrate Type M (CTM) on adherent-invasive <i>Escherichia coli</i> (AIEC) LF82, a pathobiont associated with CD. Different stages of pathogenic infection were investigated: (i) colonization of the mucus layer, and (ii) adhesion to and (iii) invasion of the epithelial cells. Following 48 h of fecal batch incubation, 0.5 and 1 mM of CTM significantly altered AIEC LF82 levels in a simulated mucus layer, resulting in a decrease of 50.5% in the untreated blank, down to 43.0% and 11.4%, respectively. At 1 mM of CTM, the significant decrease in the levels of AIEC LF82 coincided with a stimulation of the metabolic activity of the background microbiota. The increased levels of health-associated acetate (+7.9 mM) and propionate levels (+3.5 mM) suggested selective utilization of CTM by host microorganisms. Furthermore, 1 mM of both fermented and unfermented CTM decreased the adhesion and invasion of human-derived epithelial Caco-2 cells by AIEC LF82. Altogether, this exploratory in vitro study demonstrates the prebiotic potential of CTM and supports its antipathogenic effects through direct and/or indirect modulation of the gut microbiome.https://www.mdpi.com/2076-0817/10/9/1217AIECpathogencranberryin vitroadhesionmucus |
| spellingShingle | Derek Zhang Lynn Verstrepen Jelle De Medts Cindy Duysburgh Pieter Van den Abbeele Massimo Marzorati Christina Khoo A Cranberry Concentrate Decreases Adhesion and Invasion of <i>Escherichia coli</i> (AIEC) LF82 In Vitro Pathogens AIEC pathogen cranberry in vitro adhesion mucus |
| title | A Cranberry Concentrate Decreases Adhesion and Invasion of <i>Escherichia coli</i> (AIEC) LF82 In Vitro |
| title_full | A Cranberry Concentrate Decreases Adhesion and Invasion of <i>Escherichia coli</i> (AIEC) LF82 In Vitro |
| title_fullStr | A Cranberry Concentrate Decreases Adhesion and Invasion of <i>Escherichia coli</i> (AIEC) LF82 In Vitro |
| title_full_unstemmed | A Cranberry Concentrate Decreases Adhesion and Invasion of <i>Escherichia coli</i> (AIEC) LF82 In Vitro |
| title_short | A Cranberry Concentrate Decreases Adhesion and Invasion of <i>Escherichia coli</i> (AIEC) LF82 In Vitro |
| title_sort | cranberry concentrate decreases adhesion and invasion of i escherichia coli i aiec lf82 in vitro |
| topic | AIEC pathogen cranberry in vitro adhesion mucus |
| url | https://www.mdpi.com/2076-0817/10/9/1217 |
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