DNA methylation status of the cell proliferation genes in atherosclerosis
The purpose of this study was to identify the features of methylation status of genes whose products are involved in the cell proliferation in vascular tissues of patients with atherosclerosis. We tested the vascular samples from carotid arteries, internal mammary arteries and saphenous veins, which...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Siberian Branch of Russian Academy of Sciences, Research Institute of Internal and Preventive Medicine, branch of the Institute of Cytology and Genetics
2013-03-01
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| Series: | Атеросклероз |
| Subjects: | |
| Online Access: | https://ateroskleroz.elpub.ru/jour/article/view/660/597 |
| _version_ | 1797962926969061376 |
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| author | M. S. Nazarenko A. V. Markov I. N. Lebedev A. A. Sleptsov A. V. Frolov O. L. Barbarash L. S. Barbarash V. P. Puzyrev |
| author_facet | M. S. Nazarenko A. V. Markov I. N. Lebedev A. A. Sleptsov A. V. Frolov O. L. Barbarash L. S. Barbarash V. P. Puzyrev |
| author_sort | M. S. Nazarenko |
| collection | DOAJ |
| description | The purpose of this study was to identify the features of methylation status of genes whose products are involved in the cell proliferation in vascular tissues of patients with atherosclerosis. We tested the vascular samples from carotid arteries, internal mammary arteries and saphenous veins, which differ in their morphological and functional characteristics and the degree of susceptibility to pathology. DNA methylation profiling was performed by using the microarray «Infinium Human-Methylation27 BeadChip» («Illumina», USA), methylation-sensitive polymerase chain reaction and methylation-specific PCR. For the first time we identified 45 CpG-sites of 36 genes with differential DNA methylation between vascular tissues. The most pronounced differences in the DNA methylation level were registered for CpG-dinucleotides of genes ALOX12, CARD11, DAB2IP, PTPRC, RA-SIP1, THRB, TLR4, TNFRSF9 and WNT16. Our data do not support the hypothesis of epigenetic dysregulation of cell-cycle genes (CDKN2A (p16INK4a and p14ARF), CDKN2B (p15INK4b)) in atherosclerosis. |
| first_indexed | 2024-04-11T01:21:30Z |
| format | Article |
| id | doaj.art-4428497456124fa1888e7666942768d2 |
| institution | Directory Open Access Journal |
| issn | 2078-256X |
| language | Russian |
| last_indexed | 2024-04-11T01:21:30Z |
| publishDate | 2013-03-01 |
| publisher | Siberian Branch of Russian Academy of Sciences, Research Institute of Internal and Preventive Medicine, branch of the Institute of Cytology and Genetics |
| record_format | Article |
| series | Атеросклероз |
| spelling | doaj.art-4428497456124fa1888e7666942768d22023-01-03T11:21:48ZrusSiberian Branch of Russian Academy of Sciences, Research Institute of Internal and Preventive Medicine, branch of the Institute of Cytology and GeneticsАтеросклероз2078-256X2013-03-0191513DNA methylation status of the cell proliferation genes in atherosclerosisM. S. Nazarenko0A. V. Markov1I. N. Lebedev2A. A. Sleptsov3A. V. Frolov4O. L. Barbarash5 L. S. Barbarash6V. P. Puzyrev7RAMS Russian Federation SB RAMS Federal state budget institution "Research Institute of Medical Genetics"RAMS Russian Federation SB RAMS Federal state budget institution "Research Institute of Medical Genetics"RAMS Russian Federation SB RAMS Federal state budget institution "Research Institute of Medical Genetics"RAMS Russian Federation SB RAMS Federal state budget institution "Research Institute of Medical Genetics"RAMS Russian Federation SB RAMS Federal state budget institution "Research Institute of Complex Problems of Cardiovascular Diseases"RAMS Russian Federation SB RAMS Federal state budget institution "Research Institute of Complex Problems of Cardiovascular Diseases"RAMS Russian Federation SB RAMS Federal state budget institution "Research Institute of Complex Problems of Cardiovascular Diseases"RAMS Russian Federation SB RAMS Federal state budget institution "Research Institute of Medical Genetics"The purpose of this study was to identify the features of methylation status of genes whose products are involved in the cell proliferation in vascular tissues of patients with atherosclerosis. We tested the vascular samples from carotid arteries, internal mammary arteries and saphenous veins, which differ in their morphological and functional characteristics and the degree of susceptibility to pathology. DNA methylation profiling was performed by using the microarray «Infinium Human-Methylation27 BeadChip» («Illumina», USA), methylation-sensitive polymerase chain reaction and methylation-specific PCR. For the first time we identified 45 CpG-sites of 36 genes with differential DNA methylation between vascular tissues. The most pronounced differences in the DNA methylation level were registered for CpG-dinucleotides of genes ALOX12, CARD11, DAB2IP, PTPRC, RA-SIP1, THRB, TLR4, TNFRSF9 and WNT16. Our data do not support the hypothesis of epigenetic dysregulation of cell-cycle genes (CDKN2A (p16INK4a and p14ARF), CDKN2B (p15INK4b)) in atherosclerosis.https://ateroskleroz.elpub.ru/jour/article/view/660/597dna methylationatherosclerosisinfinium humanmethylation27 beadchip |
| spellingShingle | M. S. Nazarenko A. V. Markov I. N. Lebedev A. A. Sleptsov A. V. Frolov O. L. Barbarash L. S. Barbarash V. P. Puzyrev DNA methylation status of the cell proliferation genes in atherosclerosis Атеросклероз dna methylation atherosclerosis infinium humanmethylation27 beadchip |
| title | DNA methylation status of the cell proliferation genes in atherosclerosis |
| title_full | DNA methylation status of the cell proliferation genes in atherosclerosis |
| title_fullStr | DNA methylation status of the cell proliferation genes in atherosclerosis |
| title_full_unstemmed | DNA methylation status of the cell proliferation genes in atherosclerosis |
| title_short | DNA methylation status of the cell proliferation genes in atherosclerosis |
| title_sort | dna methylation status of the cell proliferation genes in atherosclerosis |
| topic | dna methylation atherosclerosis infinium humanmethylation27 beadchip |
| url | https://ateroskleroz.elpub.ru/jour/article/view/660/597 |
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