Peripheral blood microR-146a and microR-29c expression in children with Mycoplasma pneumoniae pneumonia and its clinical value
Abstract Background We investigated changes in microR-29c and microR-146a expression in the serum of children with Mycoplasma pneumoniae pneumonia, analysed their relationship with inflammatory factors and disease severity, and evaluated their diagnostic significance. Methods Fifty-six children with...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-09-01
|
| Series: | Italian Journal of Pediatrics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13052-023-01500-0 |
| _version_ | 1797557599870124032 |
|---|---|
| author | Jingcai Wang Chunyan Guo Lixin Yang Peng Sun Xiaoqing Jing |
| author_facet | Jingcai Wang Chunyan Guo Lixin Yang Peng Sun Xiaoqing Jing |
| author_sort | Jingcai Wang |
| collection | DOAJ |
| description | Abstract Background We investigated changes in microR-29c and microR-146a expression in the serum of children with Mycoplasma pneumoniae pneumonia, analysed their relationship with inflammatory factors and disease severity, and evaluated their diagnostic significance. Methods Fifty-six children with Mycoplasma pneumoniae pneumonia were enrolled as the Mycoplasma pneumoniae pneumonia group; 37 healthy children were enrolled as the control group. The microR-29c or microR-146a serum expression levels were determined using real-time quantitative reverse transcription polymerase chain reaction. Interleukin-17, tumour necrosis factor-alpha, and interleukin-1 beta levels were detected using enzyme-linked immunosorbent assay. The correlation between serum microR-29c or microR-146a expression and inflammatory factors was analysed using the Pearson’s method. Receiver operating characteristic curves were used to evaluate the diagnostic value of serum microR-29c, microR-146a, and their combined detection in Mycoplasma pneumoniae pneumonia. Results Compared with that in healthy children, the microR-29c and microR-146a serum levels were significantly downregulated in children with Mycoplasma pneumoniae pneumonia; the decrease was more obvious in children with severe cases than that in those with mild cases. In addition, microR-29c and microR-146a were negatively correlated with increased expression of interleukin-17, tumour necrosis factor-alpha, and interleukin-1 beta. Receiver operating characteristic curves showed that a combination of microR-29c and microR-146a was highly suitable for diagnosing Mycoplasma pneumoniae pneumonia. Conclusion Serum microR-29c and microR-146a were underexpressed in children with Mycoplasma pneumoniae pneumonia, and diagnostic accuracy was significantly improved with combined microR-29c and microR-146a detection. Therefore, both microR-29c and microR-146a levels can be used as biomarkers for the diagnosis of Mycoplasma pneumoniae pneumonia. |
| first_indexed | 2024-03-10T17:19:08Z |
| format | Article |
| id | doaj.art-442d030a23ee490dbe5b871fac5535a2 |
| institution | Directory Open Access Journal |
| issn | 1824-7288 |
| language | English |
| last_indexed | 2024-03-10T17:19:08Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Italian Journal of Pediatrics |
| spelling | doaj.art-442d030a23ee490dbe5b871fac5535a22023-11-20T10:23:54ZengBMCItalian Journal of Pediatrics1824-72882023-09-014911810.1186/s13052-023-01500-0Peripheral blood microR-146a and microR-29c expression in children with Mycoplasma pneumoniae pneumonia and its clinical valueJingcai Wang0Chunyan Guo1Lixin Yang2Peng Sun3Xiaoqing Jing4Department of Pediatric Medicine, Affiliated Hospital of Chengde Medical CollegeDepartment of Pediatric Medicine, Affiliated Hospital of Chengde Medical CollegeDepartment of Pediatric Medicine, Affiliated Hospital of Chengde Medical CollegeDepartment of Pediatric Medicine, Affiliated Hospital of Chengde Medical CollegeDepartment of Pediatric Medicine, Affiliated Hospital of Chengde Medical CollegeAbstract Background We investigated changes in microR-29c and microR-146a expression in the serum of children with Mycoplasma pneumoniae pneumonia, analysed their relationship with inflammatory factors and disease severity, and evaluated their diagnostic significance. Methods Fifty-six children with Mycoplasma pneumoniae pneumonia were enrolled as the Mycoplasma pneumoniae pneumonia group; 37 healthy children were enrolled as the control group. The microR-29c or microR-146a serum expression levels were determined using real-time quantitative reverse transcription polymerase chain reaction. Interleukin-17, tumour necrosis factor-alpha, and interleukin-1 beta levels were detected using enzyme-linked immunosorbent assay. The correlation between serum microR-29c or microR-146a expression and inflammatory factors was analysed using the Pearson’s method. Receiver operating characteristic curves were used to evaluate the diagnostic value of serum microR-29c, microR-146a, and their combined detection in Mycoplasma pneumoniae pneumonia. Results Compared with that in healthy children, the microR-29c and microR-146a serum levels were significantly downregulated in children with Mycoplasma pneumoniae pneumonia; the decrease was more obvious in children with severe cases than that in those with mild cases. In addition, microR-29c and microR-146a were negatively correlated with increased expression of interleukin-17, tumour necrosis factor-alpha, and interleukin-1 beta. Receiver operating characteristic curves showed that a combination of microR-29c and microR-146a was highly suitable for diagnosing Mycoplasma pneumoniae pneumonia. Conclusion Serum microR-29c and microR-146a were underexpressed in children with Mycoplasma pneumoniae pneumonia, and diagnostic accuracy was significantly improved with combined microR-29c and microR-146a detection. Therefore, both microR-29c and microR-146a levels can be used as biomarkers for the diagnosis of Mycoplasma pneumoniae pneumonia.https://doi.org/10.1186/s13052-023-01500-0miR-29cMir-146aMycoplasma pneumoniae pneumoniaInflammatory factorsdiagnostic biomarker |
| spellingShingle | Jingcai Wang Chunyan Guo Lixin Yang Peng Sun Xiaoqing Jing Peripheral blood microR-146a and microR-29c expression in children with Mycoplasma pneumoniae pneumonia and its clinical value Italian Journal of Pediatrics miR-29c Mir-146a Mycoplasma pneumoniae pneumonia Inflammatory factors diagnostic biomarker |
| title | Peripheral blood microR-146a and microR-29c expression in children with Mycoplasma pneumoniae pneumonia and its clinical value |
| title_full | Peripheral blood microR-146a and microR-29c expression in children with Mycoplasma pneumoniae pneumonia and its clinical value |
| title_fullStr | Peripheral blood microR-146a and microR-29c expression in children with Mycoplasma pneumoniae pneumonia and its clinical value |
| title_full_unstemmed | Peripheral blood microR-146a and microR-29c expression in children with Mycoplasma pneumoniae pneumonia and its clinical value |
| title_short | Peripheral blood microR-146a and microR-29c expression in children with Mycoplasma pneumoniae pneumonia and its clinical value |
| title_sort | peripheral blood micror 146a and micror 29c expression in children with mycoplasma pneumoniae pneumonia and its clinical value |
| topic | miR-29c Mir-146a Mycoplasma pneumoniae pneumonia Inflammatory factors diagnostic biomarker |
| url | https://doi.org/10.1186/s13052-023-01500-0 |
| work_keys_str_mv | AT jingcaiwang peripheralbloodmicror146aandmicror29cexpressioninchildrenwithmycoplasmapneumoniaepneumoniaanditsclinicalvalue AT chunyanguo peripheralbloodmicror146aandmicror29cexpressioninchildrenwithmycoplasmapneumoniaepneumoniaanditsclinicalvalue AT lixinyang peripheralbloodmicror146aandmicror29cexpressioninchildrenwithmycoplasmapneumoniaepneumoniaanditsclinicalvalue AT pengsun peripheralbloodmicror146aandmicror29cexpressioninchildrenwithmycoplasmapneumoniaepneumoniaanditsclinicalvalue AT xiaoqingjing peripheralbloodmicror146aandmicror29cexpressioninchildrenwithmycoplasmapneumoniaepneumoniaanditsclinicalvalue |