Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates
In vivo-matured oocytes exhibit higher developmental competence than those matured in vitro but mimicking the in vivo environment by in vitro conditions has been challenging. Until now, conventional two-dimensional (2D) systems have been used for in vitro maturation of bovine cumulus-oocytes-complex...
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/10/1635 |
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| author | Andrea Fernández-Montoro Daniel Angel-Velez Camilla Benedetti Nima Azari-Dolatabad Osvaldo Bogado Pascottini Ann Van Soom Krishna Chaitanya Pavani |
| author_facet | Andrea Fernández-Montoro Daniel Angel-Velez Camilla Benedetti Nima Azari-Dolatabad Osvaldo Bogado Pascottini Ann Van Soom Krishna Chaitanya Pavani |
| author_sort | Andrea Fernández-Montoro |
| collection | DOAJ |
| description | In vivo-matured oocytes exhibit higher developmental competence than those matured in vitro but mimicking the in vivo environment by in vitro conditions has been challenging. Until now, conventional two-dimensional (2D) systems have been used for in vitro maturation of bovine cumulus-oocytes-complexes (COCs). However, using such systems present certain limitations. Therefore, alternative low-cost methodologies may help to optimize oocyte in vitro maturation. Here, we used two different systems to culture COCs and evaluate their potential influence on embryo development and quality. In the first system, we used treated fumed silica particles to create a 3D microenvironment (liquid marbles; LM) to mature COCs. In the second system, we cultured COCs in 96-well plates with different dimensions (flat, ultra-low attachment round-bottom, and v-shaped 96-well plates). In both systems, the nuclear maturation rate remained similar to the control in 2D, showing that most oocytes reached metaphase II. However, the subsequent blastocyst rate remained lower in the liquid marble system compared with the 96-well plates and control 2D systems. Interestingly, a lower total cell number was found in the resulting embryos from both systems (LM and 96-well plates) compared with the control. In conclusion, oocytes matured in liquid marbles or 96-well plates showed no remarkable change in terms of meiotic resumption. None of the surface geometries influenced embryo development while oocyte maturation in liquid marbles led to reduced embryo development. These findings show that different geometry during maturation did not have a large impact on oocyte and embryo development. Lower embryo production after in vitro maturation in liquid marbles was probably detected because in vitro maturation was performed in serum-free medium, which makes oocytes more sensitive to possible toxic effects from the environment. |
| first_indexed | 2024-03-11T04:00:28Z |
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| language | English |
| last_indexed | 2024-03-11T04:00:28Z |
| publishDate | 2023-05-01 |
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| series | Animals |
| spelling | doaj.art-44314b4060a244cbb4693f1e4ac5af492023-11-18T00:09:58ZengMDPI AGAnimals2076-26152023-05-011310163510.3390/ani13101635Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well PlatesAndrea Fernández-Montoro0Daniel Angel-Velez1Camilla Benedetti2Nima Azari-Dolatabad3Osvaldo Bogado Pascottini4Ann Van Soom5Krishna Chaitanya Pavani6Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, 9820 Merelbeke, BelgiumDepartment of Internal Medicine, Reproduction and Population Medicine, Ghent University, 9820 Merelbeke, BelgiumDepartment of Internal Medicine, Reproduction and Population Medicine, Ghent University, 9820 Merelbeke, BelgiumDepartment of Internal Medicine, Reproduction and Population Medicine, Ghent University, 9820 Merelbeke, BelgiumDepartment of Internal Medicine, Reproduction and Population Medicine, Ghent University, 9820 Merelbeke, BelgiumDepartment of Internal Medicine, Reproduction and Population Medicine, Ghent University, 9820 Merelbeke, BelgiumDepartment of Internal Medicine, Reproduction and Population Medicine, Ghent University, 9820 Merelbeke, BelgiumIn vivo-matured oocytes exhibit higher developmental competence than those matured in vitro but mimicking the in vivo environment by in vitro conditions has been challenging. Until now, conventional two-dimensional (2D) systems have been used for in vitro maturation of bovine cumulus-oocytes-complexes (COCs). However, using such systems present certain limitations. Therefore, alternative low-cost methodologies may help to optimize oocyte in vitro maturation. Here, we used two different systems to culture COCs and evaluate their potential influence on embryo development and quality. In the first system, we used treated fumed silica particles to create a 3D microenvironment (liquid marbles; LM) to mature COCs. In the second system, we cultured COCs in 96-well plates with different dimensions (flat, ultra-low attachment round-bottom, and v-shaped 96-well plates). In both systems, the nuclear maturation rate remained similar to the control in 2D, showing that most oocytes reached metaphase II. However, the subsequent blastocyst rate remained lower in the liquid marble system compared with the 96-well plates and control 2D systems. Interestingly, a lower total cell number was found in the resulting embryos from both systems (LM and 96-well plates) compared with the control. In conclusion, oocytes matured in liquid marbles or 96-well plates showed no remarkable change in terms of meiotic resumption. None of the surface geometries influenced embryo development while oocyte maturation in liquid marbles led to reduced embryo development. These findings show that different geometry during maturation did not have a large impact on oocyte and embryo development. Lower embryo production after in vitro maturation in liquid marbles was probably detected because in vitro maturation was performed in serum-free medium, which makes oocytes more sensitive to possible toxic effects from the environment.https://www.mdpi.com/2076-2615/13/10/1635oocytein vitro maturationculture systemsassisted reproduction technologiesbovine |
| spellingShingle | Andrea Fernández-Montoro Daniel Angel-Velez Camilla Benedetti Nima Azari-Dolatabad Osvaldo Bogado Pascottini Ann Van Soom Krishna Chaitanya Pavani Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates Animals oocyte in vitro maturation culture systems assisted reproduction technologies bovine |
| title | Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates |
| title_full | Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates |
| title_fullStr | Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates |
| title_full_unstemmed | Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates |
| title_short | Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates |
| title_sort | alternative culture systems for bovine oocyte in vitro maturation liquid marbles and differentially shaped 96 well plates |
| topic | oocyte in vitro maturation culture systems assisted reproduction technologies bovine |
| url | https://www.mdpi.com/2076-2615/13/10/1635 |
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