Transcriptome and Biochemical Analyses of a Chlorophyll-Deficient Bud Mutant of Tea Plant (<i>Camellia sinensis</i>)
Tea leaf-color mutants have attracted increasing attention due to their accumulation of quality-related biochemical components. However, there is limited understanding of the molecular mechanisms behind leaf-color bud mutation in tea plants. In this study, a chlorina tea shoot (HY) and a green tea s...
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/20/15070 |
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| author | Yuanyuan Li Chenyu Zhang Chunlei Ma Liang Chen Mingzhe Yao |
| author_facet | Yuanyuan Li Chenyu Zhang Chunlei Ma Liang Chen Mingzhe Yao |
| author_sort | Yuanyuan Li |
| collection | DOAJ |
| description | Tea leaf-color mutants have attracted increasing attention due to their accumulation of quality-related biochemical components. However, there is limited understanding of the molecular mechanisms behind leaf-color bud mutation in tea plants. In this study, a chlorina tea shoot (HY) and a green tea shoot (LY) from the same tea plant were investigated using transcriptome and biochemical analyses. The results showed that the chlorophyll a, chlorophyll b, and total chlorophyll contents in the HY were significantly lower than the LY’s, which might have been caused by the activation of several genes related to chlorophyll degradation, such as <i>SGR</i> and <i>CLH</i>. The down-regulation of the <i>CHS</i>, <i>DFR</i>, and <i>ANS</i> involved in flavonoid biosynthesis might result in the reduction in catechins, and the up-regulated <i>GDHA</i> and <i>GS2</i> might bring about the accumulation of glutamate in HY. RT-qPCR assays of nine DEGs confirmed the RNA-seq results. Collectively, these findings provide insights into the molecular mechanism of the chlorophyll deficient-induced metabolic change in tea plants. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T21:12:36Z |
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| spelling | doaj.art-4431970a437c4ebea6a6f1565e53f3662023-11-19T16:41:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124201507010.3390/ijms242015070Transcriptome and Biochemical Analyses of a Chlorophyll-Deficient Bud Mutant of Tea Plant (<i>Camellia sinensis</i>)Yuanyuan Li0Chenyu Zhang1Chunlei Ma2Liang Chen3Mingzhe Yao4Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaKey Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaKey Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaKey Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaKey Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea leaf-color mutants have attracted increasing attention due to their accumulation of quality-related biochemical components. However, there is limited understanding of the molecular mechanisms behind leaf-color bud mutation in tea plants. In this study, a chlorina tea shoot (HY) and a green tea shoot (LY) from the same tea plant were investigated using transcriptome and biochemical analyses. The results showed that the chlorophyll a, chlorophyll b, and total chlorophyll contents in the HY were significantly lower than the LY’s, which might have been caused by the activation of several genes related to chlorophyll degradation, such as <i>SGR</i> and <i>CLH</i>. The down-regulation of the <i>CHS</i>, <i>DFR</i>, and <i>ANS</i> involved in flavonoid biosynthesis might result in the reduction in catechins, and the up-regulated <i>GDHA</i> and <i>GS2</i> might bring about the accumulation of glutamate in HY. RT-qPCR assays of nine DEGs confirmed the RNA-seq results. Collectively, these findings provide insights into the molecular mechanism of the chlorophyll deficient-induced metabolic change in tea plants.https://www.mdpi.com/1422-0067/24/20/15070amino acidbud mutation<i>Camellia sinensis</i>chlorophyll biosynthesisflavonoidRNA-seq |
| spellingShingle | Yuanyuan Li Chenyu Zhang Chunlei Ma Liang Chen Mingzhe Yao Transcriptome and Biochemical Analyses of a Chlorophyll-Deficient Bud Mutant of Tea Plant (<i>Camellia sinensis</i>) International Journal of Molecular Sciences amino acid bud mutation <i>Camellia sinensis</i> chlorophyll biosynthesis flavonoid RNA-seq |
| title | Transcriptome and Biochemical Analyses of a Chlorophyll-Deficient Bud Mutant of Tea Plant (<i>Camellia sinensis</i>) |
| title_full | Transcriptome and Biochemical Analyses of a Chlorophyll-Deficient Bud Mutant of Tea Plant (<i>Camellia sinensis</i>) |
| title_fullStr | Transcriptome and Biochemical Analyses of a Chlorophyll-Deficient Bud Mutant of Tea Plant (<i>Camellia sinensis</i>) |
| title_full_unstemmed | Transcriptome and Biochemical Analyses of a Chlorophyll-Deficient Bud Mutant of Tea Plant (<i>Camellia sinensis</i>) |
| title_short | Transcriptome and Biochemical Analyses of a Chlorophyll-Deficient Bud Mutant of Tea Plant (<i>Camellia sinensis</i>) |
| title_sort | transcriptome and biochemical analyses of a chlorophyll deficient bud mutant of tea plant i camellia sinensis i |
| topic | amino acid bud mutation <i>Camellia sinensis</i> chlorophyll biosynthesis flavonoid RNA-seq |
| url | https://www.mdpi.com/1422-0067/24/20/15070 |
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